Morphologic analysis of microvessels in colorectal tumors with respect to the formation of liver metastases

Yamamura, Takuya; Tsukikawa, Satoshi; Yamada, Kyoji; Yamaguchi, Susumu

doi:10.1002/jso.1164

Cited by 14 publications

(11 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, CEA mRNA expression in the blood during operation was related to blood-borne metastasis (21). According to the relation between tumor microvessels and liver metastasis, microvessels with lumens of Ͼ100 m in diameter were not detected in liver metastatic lesions (25). Moreover, (26).…”

Section: Discussionmentioning

confidence: 99%

Carcinoembryonic Antigen Messenger RNA Expression Using Nested Reverse Transcription-PCR in the Peripheral Blood During Follow-up Period of Patients Who Underwent Curative Surgery for Biliary-Pancreatic Cancer

Mataki

Takao

Maemura

et al. 2004

Clinical Cancer Research

View full text Add to dashboard Cite

Purpose: Outcome for patients with biliary-pancreatic cancer is still poor, despite curative operation. We investigated the clinical significance of molecular detection of circulating cancer cells in the blood as an early indicator of relapse during follow-up of patients who underwent a curative operation for biliary-pancreatic cancer.Patients and Methods: We followed 53 patients who underwent a curative operation for biliary-pancreatic cancer between 1996 and 2001. We used reverse transcription-PCR in the peripheral blood to evaluate carcinoembryonic antigen (CEA) mRNA expression for molecular detection of circulating cancer cells. Follow-up examinations every 3 months after surgery included CEA mRNA expression in the blood, serum CEA, serum carbohydrate antigen 19-9 (CA19-9), and computed tomography or magnetic resonance imaging.Results: Sixteen of 53 patients (30.2%) were diagnosed with a recurrence by imaging studies. The CEA mRNA detection rate in the peripheral blood of these 16 patients was 75% compared with 5.4% in the 37 patients without relapse (P < 0.001). Sensitivity of CEA mRNA, CEA, and CA19-9 serum levels was 75.0%, 50.0%, and 68.8%, respectively. Similarly, specificity was 94.6%, 64.9%, and 81.1%, respectively. CEA mRNA was expressed in the blood, even though tumor markers CEA and CA19-9 were within the normal range in patients with relapse. CEA mRNA expression in the blood, as well as the serum level of CA19-9, tended to be detected a few months earlier than detection by imaging modalities.Conclusions: During the follow-up of patients who undergo a curative operation for biliary-pancreatic cancer, CEA mRNA expression in the peripheral blood might be a useful and early indicator of relapse.

show abstract

Section: Discussionmentioning

confidence: 99%

Carcinoembryonic Antigen Messenger RNA Expression Using Nested Reverse Transcription-PCR in the Peripheral Blood During Follow-up Period of Patients Who Underwent Curative Surgery for Biliary-Pancreatic Cancer

Mataki

Takao

Maemura

et al. 2004

Clinical Cancer Research

View full text Add to dashboard Cite

show abstract

“…Upregulation of VEGF has been associated with poor prognosis in patients with colorectal cancer and linked to liver metastasis. 21,22 Hypoxia is a major inducer of VEGF activation, which occurs primarily through the transcription of hypoxia-inducible factor 1-alpha (HIF-1␣). 7 Tumor growth leads to limitations in oxygen diffusion provided by the host vasculature, creating areas of hypoxia.…”

Section: Discussionmentioning

confidence: 99%

VEGF as a predictive marker of rectal tumor response to preoperative radiotherapy

Zlobec

Steele

Compton

2005

Cancer

View full text Add to dashboard Cite

Objective Two recent analyses found that exposure to protease inhibitors (PIs) in the context of antiretroviral (ARV) therapy increased the risk for distal sensory polyneuropathy (DSPN) in subjects with human immunodeficiency virus (HIV) infection. These findings were supported by an in vitro model in which PI exposure produced neurite retraction and process loss in dorsal root ganglion sensory neurons. Confirmation of peripheral nerve toxicity with PIs could substantially limit their long‐term use in highly active ARV therapy. Methods We evaluated current and past exposure to PIs as a risk factor for DSPN in 1,159 HIV‐infected individuals enrolled in a large, prospective, observational, multicenter study. Signs of DSPN were ascertained by neurological examination. Subjects were grouped into categories according to past and current exposure to any ARV and to PIs. We included disease indicators such as nadir CD4, plasma viral load, and duration of HIV infection, as well as advancing age and exposure to dideoxynucleoside ARVs in multivariate models. Results In univariate analyses, both past and current PI exposure significantly increased the risk for DSPN. However, after adjusting for previously validated concomitant risk factors in multivariate models, none of the PI exposure groups was more likely to have DSPN than ARV naive subjects. A secondary evaluation of duration of PI use and exposure to individual PI drugs was similarly nonsignificant in multivariate models, except for small effects of amprenavir and lopinavir. Interpretation Evaluation of concomitant risks for HIV DSPN suggests that the independent risk attributable to PIs, if any, is small. This risk must be weighed against the important role of PIs in modern ARV therapy regimens. Ann Neurol 2008;64:566–572

show abstract

“…The present study reports that DEET increased MVD through a pFHHSiD sensitive pathway but in a non-significant manner. Microvessel growth is anarchic in solid tumors, producing disorganized and tortuous vessels that can vary in size59. Moreover, depending on the maturity of blood vessels, their spatial heterogeneity and their inner diameter, MVD does not systematically correlate with functional vascularity.…”

Section: Discussionmentioning

confidence: 99%

The insect repellent N,N-diethyl-m-toluamide (DEET) induces angiogenesis via allosteric modulation of the M3 muscarinic receptor in endothelial cells

Legeay

Clere

Hilairet

et al. 2016

Sci Rep

View full text Add to dashboard Cite

The insect repellent N,N-diethyl-m-toluamide (DEET) has been reported to inhibit AChE (acetylcholinesterase) and to possess potential carcinogenic properties with excessive vascularization. In the present paper, we demonstrate that DEET specifically stimulates endothelial cells that promote angiogenesis which increases tumor growth. DEET activates cellular processes that lead to angiogenesis including proliferation, migration and adhesion. This is associated with an enhancement of NO production and VEGF expression in endothelial cells. M3 silencing or the use of a pharmacological M3 inhibitor abrogates all of these effects which reveals that DEET-induced angiogenesis is M3 sensitive. The experiments involving calcium signals in both endothelial and HEK cells overexpressing M3 receptors, as well as binding and docking studies demonstrate that DEET acts as an allosteric modulator of the M3 receptor. In addition, DEET inhibited AChE which increased acetylcholine bioavailability and binding to M3 receptors and also strengthened proangiogenic effects by an allosteric modulation.

show abstract

Morphologic analysis of microvessels in colorectal tumors with respect to the formation of liver metastases

Abstract: Large microvessels in the primary tumor favor intravasation of cancer cells.

Cited by 14 publications

References 25 publications

Carcinoembryonic Antigen Messenger RNA Expression Using Nested Reverse Transcription-PCR in the Peripheral Blood During Follow-up Period of Patients Who Underwent Curative Surgery for Biliary-Pancreatic Cancer

Carcinoembryonic Antigen Messenger RNA Expression Using Nested Reverse Transcription-PCR in the Peripheral Blood During Follow-up Period of Patients Who Underwent Curative Surgery for Biliary-Pancreatic Cancer

VEGF as a predictive marker of rectal tumor response to preoperative radiotherapy

The insect repellent N,N-diethyl-m-toluamide (DEET) induces angiogenesis via allosteric modulation of the M3 muscarinic receptor in endothelial cells

Contact Info

Product

Resources

About