Morphokinetics of Blastocyst Expansion in the Mouse is Similar to Human Embryos: An Embryoscope Study

Chinn, Kathryn; Huang, Tian-Sen

doi:10.1016/j.fertnstert.2014.12.043

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(2 citation statements)

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“…The data presented further suggest that the zona pellucida modulates the underlying regularity in both the periodicity and amplitude of oscillations as previously shown in expanding zona-free mouse blastocysts (Chinn and Huang, 2015). Theoretically, the zona might variably affect expan- Testing r = 0 under null hypothesis (P > 0.05 indicates that r is not significantly different from zero).…”

Section: Cellular Perspective: On the Origin And Possible Significance Of Pulsing Oscillatory-like Behaviour Characterizing Human Blastocsupporting

confidence: 81%

“…In addition to overall expansion rates, this study draws a distinction between two types of pulsatory or oscillatory-like motions during blastocyst expansion. The most common is associated with relatively uninterrupted expansion (thus termed E-type), where the hourly interval rate of change values are generally positive in the human, whereas they are sometimes slightly negative in the mouse (Chinn and Huang, 2015). The physiological mechanism(s) underlying this rhythmicity remain to be identified.…”

Section: Cellular Perspective: On the Origin And Possible Significance Of Pulsing Oscillatory-like Behaviour Characterizing Human Blastocmentioning

confidence: 99%

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Morphokinetics of human blastocyst expansion in vitro

Huang

Chinn

Kosasa

et al. 2016

Reproductive BioMedicine Online

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Time-lapse imaging offers new tools to study dynamic processes of development such as blastocyst formation and expansion. This study quantitatively describes expansion in human blastocysts from donated oocytes. Measurements of hourly interval rate of changes in the blastocoel cross-sectional area revealed oscillatory pulses having 2-4 h periodicities. Two types of oscillations were distinguished. An E-Type ('expansion') had positive peak and positive or slightly negative trough interval rate of change values, and these characterized most of the expansion period. A C-type ('contraction') represented an infrequent but notable contraction of the blastocoel with loss of blastocoel fluid. These were reversible within 2-4 h in both groups and followed by further expansion. Therefore, oscillatory pulses are an intrinsic property of the trophectoderm. The zona seems to variably dampen the amplitude of these pulses. Expansion kinetics were compared between blastocysts with known positive (KID+) or negative (KID-) implantation outcomes. Regression analysis suggests that expansion may be relatively restricted in KID- embryos blastulating at relatively later times. These data extend observations in other mammalian systems and may provide information useful for clinical selection algorithms.

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Section: Cellular Perspective: On the Origin And Possible Significance Of Pulsing Oscillatory-like Behaviour Characterizing Human Blastocsupporting

confidence: 81%

Section: Cellular Perspective: On the Origin And Possible Significance Of Pulsing Oscillatory-like Behaviour Characterizing Human Blastocmentioning

confidence: 99%