Morphogenic Events in Ferns: Single and Multicellular Explants In Vitro

Rybczyński, Jan J.; Tomiczak, Karolina; Grzyb, Małgorzata; Mikuła, Anna

doi:10.1007/978-3-319-75103-0_5

Cited by 10 publications

(7 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, distinct and adapted cryogenic techniques have been applied to a wide range of tissues, such as embryogenic tissue cultured in a liquid medium and independent-living autotrophic fern gametophytes, which are capable of sexual reproduction and vegetative propagation, especially under in vitro culture conditions (Rybczyński et al, 2018). Efficient in vitro regeneration systems developed at the Department of Plant Conservation Biology of the PAS BG-CBDC (Rybczyński et al, 2015(Rybczyński et al, , 2018 have provided sufficient tissue for experimental purposes, thus enriching our understanding and enabling the development of highly efficient cryopreservation protocols (Makowski et al, 2015(Makowski et al, , 2016Mikuła et al, 2011Mikuła et al, , 2015Tomiczak et al, 2018). is has allowed for the embryogenic tissues of four species of the genus Gentiana to be introduced into LN and stored successfully for 11 years (Table 1).…”

Section: The Polish Academy Of Sciences Botanical Garden -Center For ...mentioning

confidence: 99%

Polish Cryobanks: Research and Conservation of Plant Genetic Resources

et al. 2022

Self Cite

View full text Add to dashboard Cite

To date, the use of liquid nitrogen (LN) in plant gene banks is relatively limited. Globally, in 2021, approximately 10,000 accessions were cryopreserved, and their explants were derived from in vitro plants. In Europe, out of 500 banks, only 20 use cryogenic temperatures to store plant germplasms. The implementation of seven conservation projects in Poland starting in the 1990s meant that the gene banking system in this country began to gradually develop. Plant gene bank is mainly based on the collection of seeds and makes little use of plant tissues, including those from in vitro culture. From the point of view of systematics, plant material belonging to spermatophytes dominates in Polish gene banks, while spore plants are rarely represented. This review represents the first summary of gene cryobank activities and resources in Poland. It contains a brief overview of the ex situ plant protection programs aimed at the use of LN and presents the characteristics of four existing gene cryobanks in this country. Special attention is devoted to the presentation of studies on the cryopreservation of seeds, as well as of plant tissues, such as embryonic axes and plumules of trees, and fern gametophytes, that make a permanent contribution to gene bank resources.

show abstract

Section: The Polish Academy Of Sciences Botanical Garden -Center For ...mentioning

confidence: 99%

Polish Cryobanks: Research and Conservation of Plant Genetic Resources

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, many phenolics and antioxidant enzymes were found in the liquid medium of fern cell suspension cultures [ 29 , 30 ]. The limited knowledge of the production of active compounds under in vitro conditions found in cryptogamous species is mainly due to a poor understanding of their biological potential, that could be discovered with the help of current biotechnological tools [ 31 ]. Thus, the aim of our experiments was to establish the cell suspension culture of Cyathea delgadii , and to describe the chemical composition of the post-culture medium as an expression of intensive cell metabolism occurring during a two-week long subculture.…”

Section: Introductionmentioning

confidence: 99%

The Metabolite Content of the Post-Culture Medium of the Tree Fern Cyathea delgadii Sternb. Cell Suspension Cultured in the Presence of 2,4-D and BAP

Rybczyński

Marczak

Stobiecki

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

The aim of this study was to demonstrate the metabolic profile of post-culture medium as an expression of cell suspension metabolic activity of the tree fern Cyathea delgadii Sternb. The molecular profile of the tree fern’s cell culture has been never described, according to our knowledge. The cell suspension was established using ½MS medium supplemented with various concentrations of 2,4-D and BAP. The optimal concentrations were 2.0 mg·L-1 and 0.2 mg·L-1, respectively. The cell suspension initially showed an organized system of cell division and later unorganized cell proliferation. LC-MS and GC-MS were used to identify the chemical composition of the post-culture medium. The LC-MS analysis results suggested that the color of liquid medium could be due to the presence of flavonoid derivatives, as this group of compounds was represented by eight compounds. After GC-MS analysis based on retention indexes and thanks to mass spectra comparison, 130 natural products were recognized, belonging to various classes of primary and secondary metabolites.

show abstract

“…On the other hand, phytohormones take part in the regulation of almost all phases of plant development, and also mediate the responses to various environmental stresses (Hicks, 1894 ; Rademacher, 2000 ; Dobrev et al, 2002 ). In ferns, growth and development of gametophyte and sporophyte are controlled by plant growth regulators, receiving especial attention their applications on morphogenesis, and its great repercussion on the sporophyte multiplication of ornamental ferns (Amaki and Higuchi, 1992 ; Fernández and Revilla, 2003 ; Somer et al, 2010 ; Rybczynski et al, 2018 ; Singh and Johari, 2018 ). The impact of phytohormones on gametophyte reproduction has been scrutinized in several species, some of them governed by an antheridiogen system, such as B. spicant , being these compounds devoted to promote genetic exchange, and linked chemically to gibberellin-related diterpenoids (Yamane, 1998 ; Menéndez et al, 2006a , b ; Kazmierczak, 2010 ; Tanaka et al, 2014 ; Valledor et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Sexual and Apogamous Species of Woodferns Show Different Protein and Phytohormone Profiles

et al. 2021

View full text Add to dashboard Cite

The gametophyte of ferns reproduces either by sexual or asexual means. In the latter, apogamy represents a peculiar case of apomixis, in which an embryo is formed from somatic cells. A proteomic and physiological approach was applied to the apogamous fern Dryopteris affinis ssp. affinis and its sexual relative D. oreades. The proteomic analysis compared apogamous vs. female gametophytes, whereas the phytohormone study included, in addition to females, three apogamous stages (filamentous, spatulate, and cordate). The proteomic profiles revealed a total of 879 proteins and, after annotation, different regulation was found in 206 proteins of D. affinis and 166 of its sexual counterpart. The proteins upregulated in D. affinis are mostly associated to protein metabolism (including folding, transport, and proteolysis), ribosome biogenesis, gene expression and translation, while in the sexual counterpart, they account largely for starch and sucrose metabolism, generation of energy and photosynthesis. Likewise, ultra-performance liquid chromatography-tandem spectrometry (UHPLC-MS/MS) was used to assess the levels of indol-3-acetic acid (IAA); the cytokinins: 6-benzylaminopurine (BA), trans-Zeatine (Z), trans-Zeatin riboside (ZR), dyhidrozeatine (DHZ), dyhidrozeatin riboside (DHZR), isopentenyl adenine (iP), isopentenyl adenosine (iPR), abscisic acid (ABA), the gibberellins GA3 and GA4, salicylic acid (SA), and the brassinosteroids: brassinolide (BL) and castasterone (CS). IAA, the cytokinins Z, ZR, iPR, the gibberellin GA4, the brassinosteoids castasterone, and ABA accumulated more in the sexual gametophyte than in the apogamous one. When comparing the three apogamous stages, BA and SA peaked in filamentous, GA3 and BL in spatulate and DHRZ in cordate gametophytes. The results point to the existence of large metabolic differences between apogamous and sexual gametophytes, and invite to consider the fern gametophyte as a good experimental system to deepen our understanding of plant reproduction.

show abstract

Morphogenic Events in Ferns: Single and Multicellular Explants In Vitro

Cited by 10 publications

References 67 publications

Polish Cryobanks: Research and Conservation of Plant Genetic Resources

Polish Cryobanks: Research and Conservation of Plant Genetic Resources

The Metabolite Content of the Post-Culture Medium of the Tree Fern Cyathea delgadii Sternb. Cell Suspension Cultured in the Presence of 2,4-D and BAP

Sexual and Apogamous Species of Woodferns Show Different Protein and Phytohormone Profiles

Contact Info

Product

Resources

About