Morphogenetische Untersuchungen an in vitro wachsenden Augenanlagen vonDrosophila melanogaster

Gottschewski, Georg

doi:10.1007/bf00649886

Cited by 39 publications

(5 citation statements)

References 15 publications

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“…We therefore chose MM3 medium for further studies. Cultures grown in MM3 and L-15 media developed a cuticle-like covering that formed over the eye disc fragment, as previously observed by Gottschewski ( 1960) in cultures using his own medium. One further difference between the two media was that in L-I5 this covering formed one or more large vesicles that bulged outward from the fragment.…”

Section: Culture Mediasupporting

confidence: 81%

Conditions for the primary culture of eye imaginal discs from Drosophila melanogaster

Li¹,

Meinertzhagen²

1995

J. Neurobiol.

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We have established a primary culture system for Drosophila eye imaginal discs. With this system, we were able to obtain neurite outgrowth from intact eye discs, eye disc fragments, and dissociated eye imaginal disc cells. Immunoreactivity to antibody 24B10 indicates that these extending neurites are photoreceptor axons. Three culture media were tested for their ability to support the survival of and neurite extension from eye disc fragments in vitro at 23 degrees C. These, with supplements, were: five parts of Schneider's Drosophila medium with four parts of basal Eagle's medium ("4 + 5"); Leibovitz's L-15 medium (L-15); and Shields and Sang's M3 modified medium (MM3). We obtained the best results with MM3 supplemented with 2% fetal bovine serum (FBS). Eye disc fragments survived in this medium for at least 20 days. Pigmentation in the nonphotoreceptor pigment cells in cultures from the prepupa required the presence of 20-hydroxyecdysone (20-HE) (1 micrograms/ml), whereas neurite outgrowth was seen in the absence of 20-HE. Donor animals had to fall within a range of ages to obtain appropriate eye disc differentiation in vitro. Eye disc from 5-h pupae (P + 5) or older commenced ommachrome synthesis in vitro in a temporal sequence close to that found in vivo, whereas the in vitro synthesis of this pigment was delayed in eye discs from younger flies. Average neurite length was not affected by age among pupae younger than P + 5; but neurite outgrowth from P + 24 was scarce, probably because by this time photoreceptor axons had already grown in vivo and were severed and unable to regenerate in vitro. Eye discs taken from third instar larvae or white prepupae continued their mitotic activity in vitro. Together with the advance of the morphogenetic furrow at the leading edge of retinal development, this observation is consistent with the evidence that pattern formation continues in vitro. Morphogenetic changes were manifested in cultures. Viability tests with calcein AM and ethidium bromide revealed few dead cells in living cultures.

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Section: Culture Mediasupporting

confidence: 81%

Conditions for the primary culture of eye imaginal discs from Drosophila melanogaster

Li¹,

Meinertzhagen²

1995

J. Neurobiol.

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“…Eye-antennal discs By 1960, the field of ring gland endocrinology had, of necessity, moved to in vitro co-culture formats. Gottschewski (1960) reported that the eye-antennal disc would develop in culture only in the presence of the ring gland. Schneider (1964) preconditioned the culture medium for 24 h with ring glands from 3rd instar larvae that were 2 h prior to becoming immobile (stage L1; Bainbridge and Bownes, 1981;see Fig.…”

Section: Endocrine Activities Of Larval Corpora Allatal Cells: Summarymentioning

confidence: 98%

Farnesoid secretions of dipteran ring glands: What we do know and what we can know

Jones

2007

Insect Biochemistry and Molecular Biology

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“…The medium had a pH of 7.3 and osmolality of 900 mosmol (Bayer, 1975). The concentrations of retinal and tocopherol are derived from the tissue culture studies of the ocular discs of Drosophila (Gottschewski, 1960). The glucose and penicillin-streptomycin concentrations are those used by Wolff (1963) for studying the structural integrity of explanted Limulus organs.…”

Section: Composition Of Bathing Mediamentioning

confidence: 99%

Limulus ventral eye. Physiological properties of photoreceptor cells in an organ culture medium.

Bayer¹,

Barlow²

1978

The Journal of General Physiology

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A B S TRA C T Ventral photoreceptor cells bathed in an organ culture medium typically have resting potentials of -85 mV and membrane resistances of 35 M12 and, when dark-adapted, exhibit large potential fluctuations (LPFs) of 60 mV and small potential fluctuations (SPFs) of <30 mV. LPFs appear to be regenerative events triggered by SPFs, the well-known quantum humps. In the dark, SPFs and LPFs occur spontaneously. At intensities near threshold, the rate of occurrence is directly proportional to light intensity, indicating that SPFs and LPFs are elicited by single photon events. At higher intensities, SPFs and LPFs sum to produce a receptor potential that is graded over approximately a 9-log-unit range of light intensity. Amplitude histograms of the discrete potential waves are bimodal, reflecting the SPF and LPF populations. Histograms of current waves are unimodal. SPFs and LPFs are insensitive to 1 /zM tetrodotoxin. I-V characteristics show initial inward currents of -15 nA for voltage clamps to -40 mV and steady-state outward currents for all clamp potentials. Photoreceptor cells bathed in organ culture medium retain these properties for periods of at least 75 days.

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Morphogenetische Untersuchungen an in vitro wachsenden Augenanlagen vonDrosophila melanogaster

Cited by 39 publications

References 15 publications

Conditions for the primary culture of eye imaginal discs from Drosophila melanogaster

Conditions for the primary culture of eye imaginal discs from Drosophila melanogaster

Farnesoid secretions of dipteran ring glands: What we do know and what we can know

Limulus ventral eye. Physiological properties of photoreceptor cells in an organ culture medium.

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