Morphogenetic potential and in vitro micropropagation of endangered plant species Leucojum aestivum L. and Lilium rhodopaeum Delip.

Stanilova, Marina; Ilcheva, V.; Загорска, Н.

doi:10.1007/bf00231965

Cited by 30 publications

(17 citation statements)

References 3 publications

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“…Leaves appeared on some of them. The morphological changes and patterns for this variety were similar to those observed in Lilium rhodopaeum Delip (Stanilova et al 1994). The small bulblets grew rapidly when they were transferred to the culture bulblet medium.…”

Section: Establishment Of Sterile Culturesupporting

confidence: 68%

In vitro mutagenesis and identification of mutants via ISSR in lily (Lilium longiflorum)

et al. 2012

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An efficient in vitro mutagenesis protocol for Lilium longiflorum Thunb. cv. White fox has been established. The effect of 6-BA and NAA on adventitious bud formation from the bulblet-scale thin cell layers was tested. Results showed that the optimal medium for adventitious bud induction is MS basal medium supplemented with 2.0 mg/l 6-BA and 0.1 mg/l NAA. The differentiation frequency and the average number of adventitious buds reached 95.55% and 3.00, respectively. Various doses (0.0, 0.5, 1.0, 1.5, 2.0, and 2.5 Gy) of gamma rays were applied to investigate the effect of radiation on adventitious bud formation from bulblet-scale thin cell layers. The forming capacity of the adventitious buds significantly decreased with the increase of radiation dose. The results suggested that the optimal irradiation dose is 1.0 Gy. Dose of 1.0 Gy treatment resulted in 55.33% survival of irradiated bulblet-scale thin cell layers and 39.27% mutagenesis rate. The genetic variations among the morphological mutants were evaluated by DNA fingerprinting using ISSR molecular marker. The genetic variation frequency reached 36.06% using seven ISSR primers. Out of the 50 mutant lines transferred to the greenhouse, 9 were observed to have significantly different morphological characters than those of the controls.

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Section: Establishment Of Sterile Culturesupporting

confidence: 68%

In vitro mutagenesis and identification of mutants via ISSR in lily (Lilium longiflorum)

et al. 2012

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“…The higher number of bulblets and roots obtained per explants were respectively 1.5 and 5.6 (Diop et al 2006). Stanilova et al (1994) used also NAA-containing medium to directly induce bulblets in L. aestivum cultures.…”

Section: Tissue Culturesmentioning

confidence: 99%

Hairy root and tissue cultures of Leucojum aestivum L.—relationships to galanthamine content

Diop¹,

Hehn²,

Ptak

et al. 2007

Phytochem Rev

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Galanthamine, an isoquinoline alkaloid acetylcholinesterase inhibitor, is an important agent used all around the world for the symptomatic treatment of senile dementia of the Alzheimer's type. The production of this metabolite and the availability of the plant are limited and prompted the search for an alternative way to obtain this valuable metabolite using in vitro cultures of Leucojum aestivum L. It is known that cell differentiation level shows a major influence upon the accumulation of alkaloids. For this reason, tissue cultures of L. aestivum showing different stages of morphogenesis controlled by exogenous growth regulators were established. Agrobacterium rhizogenes strain LBA 9402 has been tested for its capacity to induce hairy roots of this monocotyledonae plant.

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“…One approach to overcoming these limitations is somatic embryogenesis (SE), which is one of the fastest methods for micropropagating whole plants. As such, SE is a highly valued plant culture technique (Fay 1992;Stanilova et al 1994;Karami et al 2008). The main advantage of SE compared to the other regeneration methods, such as organogenesis, is that it produces a functional meristem with a vascular system and a root/shoot axis in a single step (Bassuner et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration of Lilium ledebourii (Baker) Boiss., an endangered species

Bakhshaie

Babalar

Mirmasoumi

et al. 2010

Plant Cell Tiss Organ Cult

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A somatic embryogenesis (SE) protocol was established for the regeneration of Lilium ledebourii (Baker) Boiss. whole plants using new vegetative bulblet microscales and transverse thin cell layers (tTCLs) of young bulblet roots as the explant sources. Bulblets were induced from bulb scale explants cultured for at least 3 months in the dark on Murashige and Skoog (MS) medium containing 3% sucrose, 0.8% agar, and different concentrations of a-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), and thidiazuron. Embryo-like structures were obtained from tTCL explants of 3-month-old bulblets (excised from bulb scale explants) following culture on solid MS medium containing 3% sucrose and various concentrations of NAA and BA for 3 months in the dark. Both the explant source and the type of plant growth regulators affected the differentiation of somatic embryos. The highest percentage (65.55%) of embryogenesis was obtained from bulblet microscale tTCLs cultured on solid MS medium containing 0.54 lM NAA and 0.44 lM BA. Plants with normal shoots and roots were obtained following a 3-month culture of embryos on growth regulatorfree MS medium at 25 ± 1°C under a 16/8-h light/dark photoperiod (light intensity 40 lmol m -2 s -1 , cool-white fluorescent light). The plants were successfully acclimatized in the growth chamber.

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Morphogenetic potential and in vitro micropropagation of endangered plant species Leucojum aestivum L. and Lilium rhodopaeum Delip.

Cited by 30 publications

References 3 publications

In vitro mutagenesis and identification of mutants via ISSR in lily (Lilium longiflorum)

In vitro mutagenesis and identification of mutants via ISSR in lily (Lilium longiflorum)

Hairy root and tissue cultures of Leucojum aestivum L.—relationships to galanthamine content

Somatic embryogenesis and plant regeneration of Lilium ledebourii (Baker) Boiss., an endangered species

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