Morphogenesis of bovine parvoviruses and associated cellular changes

Bates, Robert C.; Storz, J.; Doughri, A.M.

doi:10.1016/0014-4800(74)90055-0

Cited by 15 publications

(5 citation statements)

References 8 publications

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“…The focal pattern of nuclear labelling suggests that viral encapsidation takes place at certain discrete sites. The finding that antigen was not dispersed throughout the central 'cleared' area of infected nuclei confirmed that margination of chromatin was not merely due to displacement by accumulating capsids (as suggested by Bates et al (1974) for bovine parvovirus), but was probably due to an active cytopathic effect of the virus.…”

Section: Discussionsupporting

confidence: 50%

Combined immunocytochemistry and non-isotopic in situ hybridization for the ultrastructural investigation of human parvovirus B19 infection

1995

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Parvovirus B19 is a single-stranded DNA virus with a specific tropism for human erythroid precursor cells. The virus codes for two overlapping structural (capsid) proteins and one non-structural protein which is thought to perform essential functions in viral replication, transcription and packaging. The ultrastructural localization of these proteins was achieved in cultured haemopoietic cells derived from fetal liver which had been infected in vitro and subsequently embedded in LR White acrylic resin. Postembedding immunogold detection of B19 structural and non-structural proteins was combined with localization of viral nucleic acid by in situ hybridization using a digoxigenin-labelled probe and different sized gold labels. The majority of the B19 capsid protein and DNA present in cells harvested 48 hours post-infection co-localized within the centri-nuclear region of erythroid cells demonstrating characteristic chromatin margination. Relatively little DNA hybridization signal was present over paracrystalline inclusions strongly labelled with anti-capsid protein monoclonal antibody R92F6. Viral DNA and capsid protein were co-localized in apparent egress from the nucleus through nuclear pores. B19 non-structural protein was detected in association with both nuclear and cytoplasmic arrays of capsids, supporting the view that this protein plays an important role in viral packaging and remains associated with the complete viral particle until its release from the cell. Co-localization of viral nucleic acid and proteins at the ultrastructural level is a flexible, rapid and highly specific tool for examination of viral life-cycles within cells.

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Section: Discussionsupporting

confidence: 50%

Combined immunocytochemistry and non-isotopic in situ hybridization for the ultrastructural investigation of human parvovirus B19 infection

1995

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“…The first ultrastructural changes are generally observed 6-12 hours postinfection (PI) (Singer and Toolan, 1975;Garzon and Kurstak, 1976). Bates et al (1974), who studied the morpho genesis in BPV, also found small particles in association with the smooth endoplasmic reticulum and suggested that they were virions. Bates et al (1974), who studied the morpho genesis in BPV, also found small particles in association with the smooth endoplasmic reticulum and suggested that they were virions.…”

Section: Ultrastructural Changes Related To Viral Developmentmentioning

confidence: 99%

Animal Parvoviruses: Comparative Aspects and Diagnosis

Kurstak¹,

Tijssen²

1981

Vertebrate Animal and Related Viruses

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“…Ultrahistologische Befunde iiber die Verrnehrung von autonomen Parvoviren in infizierten Zellen wurden vergleichsweise wenig publiziert. Beschrieben und elektronenoptisch dargestellt wurde die Reifung der Parvoviren im Zellkern iiberwiegend in Zentren mit semi-oder parakristalliner Partikelformation, aber auch mit diffuser Verteilung, beim bovinen (12), porcinen (5), caninen (7) und felinen ( 6 ) Parvovirus sowie beim KBSH-(9) und H-1-Virus (10 (4). Solche Partikeln auRerhalb von Kernen sichteten wir in BPV-infizierten BEL-Zellen ebenfalls (Abb.…”

unclassified

Zur Morphologie des bovinen Parvovirus

Mahnel¹

1988

Journal of Veterinary Medicine, Series B

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Summary On the morphogenesis of bovine parvovirus The morphogenesis of bovine parvovirus is described on ultrathin sections of BEL cells. Virus particles are maturing first in the nucleus at the periphery of disseminated areas with fine filaments, merging to few larger centres with crystal‐like arrangement of virions occupying great parts of the nucleus. Zusammenfassung Anhand elektronenoptischer Aufnahmen von Ultradünnschnitten wird die Morphogenese boviner Parvoviren in BEL‐Zellen beschrieben. Viruspartikel reifen zuerst an diffus verteilten kleinen Zonen mit feinen Filamenten, die zu wenigen größeren, große Teile des Zellkerns einnehmenden Zentren mit kristallartig angeordneten Viren zusammenfließen.

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Morphogenesis of bovine parvoviruses and associated cellular changes

Cited by 15 publications

References 8 publications

Combined immunocytochemistry and non-isotopic in situ hybridization for the ultrastructural investigation of human parvovirus B19 infection

Combined immunocytochemistry and non-isotopic in situ hybridization for the ultrastructural investigation of human parvovirus B19 infection

Animal Parvoviruses: Comparative Aspects and Diagnosis

Zur Morphologie des bovinen Parvovirus

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