Morphogenesis in Tissue Culture of Local Kazakh Cotton Varieties

Nazira, Bishimbayeva; Bakhyt, Yertayeva; Aigul, Amirova; Izbasar, Rakhimbayev

doi:10.3844/ojbsci.2017.178.186

Cited by 2 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sterilized seeds were cultivated in three germination media without mineral salts. The media were composed to 6% of gelose melted in distilled water with different concentration of gibberellic acid (GA 3 : 0 mg/l; 0.5 mg/l, and 1 mg/l) follow by regular observation frequency of three days after initiation [10]. The explants regeneration and growth phase was carried out on nine Murashigue and Skoog [11] (MS) modified media different in their concentration of auxins and cytokinins (M0, M1, M2, M3, M4 M5, M6, M7 and M8) contained in common 30 g/l of sucrose, GA 3 (1mg/l) and gelose (7g/l) [5].…”

Section: Culture Conditions and Hormonal Treatmentsmentioning

confidence: 99%

In vitro Organogenesis in Cotton (Gossypium spp) for Ex-situ Conservation Issue

Sinha

Cacaï²,

Houngue³

et al. 2021

ARRB

View full text Add to dashboard Cite

Background: Monovarietal cultivation of cotton plant allowed the genetic erosion for traditional cotton varieties that proceed essential traits mainly involved in cotton genetic breeding. These varieties need to be preserved for future used. This study aims to evaluate the effect of gibberellic acid on cotton seed germination and the effect of Benzylaminopurin (BAP), Kinetin (KIN), α-naphthalene acetic acid (NAA) and activated charcoal (CA) on cotton seedlings growth obtained from different type of explants. Methodology: The seeds of three improved varieties (KET782, ANG956, OKP768) and five local varieties (Q62, Q64, Q85, Q88, Q92) were germinated with different concentration of gibberellic acid (GA3). Different explants were collected from the germinated plantlets and cultivated in different culture media containing plants growth regulators and activated charcoal in different concentration. Results: We found that Gibberellic Acid activity on cotton seeds germination significantly varied according to the variety and GA3 concentration in the medium. 63.33% germination rate in OKP768 was obtained on the medium containing 0.5 mg/L of GA3 while the medium with 1 mg/l of GA3 gave the highest seed germination in Q85 (75%), Q64 (69.17%), and ANG956 (40.83%). The plantlets regeneration rate varied within the explants in different varieties used. 100%of axillary nodes were regenerated in Q62; Q85 and Q92 varieties while 60% were regenerated with the zygotic embryos in ANG956, and OKP768. Moreover, the medium supplemented with GA3 (1mg/l) + NAA (1 mg/l) induced the greatest number of roots (2.75 roots/plantlet) in the ANG956 and OKP768 varieties. With activated charcoal (10 g/l), ANG956 and OKP768 varieties achieved better performance with respective roots length average of 3.4 cm/plantlet and 2.1 cm/plantlet. The activated charcoal at 10 g/l highly influenced the length of roots with an average of 7.7cm in ANG956 variety. Implication: The protocol established during this study will be useful for in vitro regeneration and conservation for cotton local varieties.

show abstract

Section: Culture Conditions and Hormonal Treatmentsmentioning

confidence: 99%

In vitro Organogenesis in Cotton (Gossypium spp) for Ex-situ Conservation Issue

Sinha

Cacaï²,

Houngue³

et al. 2021

ARRB

View full text Add to dashboard Cite

show abstract

Multiple Plant Regeneration from Embryogenic Calli of Paulownia tomentosa (Thunb.) Steud

Aigul

Dossymbetova

Rysbayeva

et al. 2022

Plants

View full text Add to dashboard Cite

The aim of this paper was to study the effect of plant growth regulators on callus induction and in vitro morphogenesis using various explants of Paulownia tomentosa to develop an efficient plant regeneration protocol. Different plant organ sections (leaves, apical shoot tips, petals, nodes, and internodes) were cultured as explants to identify the best in vitro explants responsive to callus induction and plant regeneration. Explants were cultivated on MS media supplemented with different concentrations of plant growth regulators (TDZ (Thidiazuron), BAP (6-Benzylaminopurine), kinetin, and NAA (1-Naphthaleneacetic acid). It was discovered that the addition of TDZ and NAA stimulated the induction of somatic embryogenesis. It was discovered that the MS medium with the combination of plant growth regulators BAP (35.5 µM) and NAA (5.4 µM) with the addition of 30.0 g/L maltose, 500.0 mg/L casein hydrolysate, and 250.0 mg/L L-proline was optimal for callus induction and multiple plant regeneration. The study of the regenerative capacity of various explants of Paulownia tomentosa in vitro showed that plant regeneration depends on the type of explant, and occurs in both ways, indirectly, through the formation of callus tissues and directly on the explant, without callus formation. As a result of this study, the efficient reproducible protocol of embryogenic callus formation and multiple shoot induction in vitro of Paulownia tomentosa was developed. This system provides a clear increase in the frequency of plant regeneration from 36.3 ± 3.4% to 38.6 ± 2.3% per embryogenic callus from leaves and apical shoot tips, respectively.

show abstract

Morphogenesis in Tissue Culture of Local Kazakh Cotton Varieties

Cited by 2 publications

References 14 publications

In vitro Organogenesis in Cotton (Gossypium spp) for Ex-situ Conservation Issue

In vitro Organogenesis in Cotton (Gossypium spp) for Ex-situ Conservation Issue

Multiple Plant Regeneration from Embryogenic Calli of Paulownia tomentosa (Thunb.) Steud

Contact Info

Product

Resources

About