Morphogenesis in leaf and single-cell cultures of mature Juniperus oxycedrus

Gómez, María Pilar Garcés; Segura, Juan

doi:10.1093/treephys/16.8.681

Cited by 14 publications

(12 citation statements)

References 25 publications

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“…Gomez and Segura 1995, Andersone and Ievinsh 2005 few information is available in Juniperus species. Gomez and Segura (1995) reported the proliferation of J. oxycedrus by axillary shoot proliferation and later found some morphogenic capacity in calluses derived from single cell culture of the same species (Gomez and Segura 1996). More recently, Shanjani (2003) highlighted the importance of nitrogen on callus induction and plant regeneration of J. excelsa, and recently new media formulation based on the explant mineral composition are being proposed as a new strategy for highly recalcitrant species (e.g.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Juniperus phoenicea from adult plant explants and analysis of ploidy stability using flow cytometry

Loureiro¹,

Capelo²,

Brito³

et al. 2007

Biol. plant.

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We report here the successful micropropagation of adult Juniperus phoenicea L. with respective ploidy stability studies. Microcuttings with axillary buds were grown on five media supplemented with different growth regulator combinations. Best elongation rates were achieved on Driver and Kuniyuki (DKW) medium supplemented with kinetin alone or with naphthaleneacetic acid (NAA), while Rugini olive (OM) medium stimulated the development of new branches. Shoots growing on Murashige and Skoog (MS) medium browned and showed necrotic zones. Shoots of second to fourth subcultures usually had higher elongation rates than those of the first culture. For rooting assays, half strength DKW and OM media, different concentrations of growth regulators, auxin continuous exposure vs. dipping and the type of solid matrix were assessed. During rooting assays, two morphotypes were observed with one type having well developed internodes and the other showing hyperhydratation and no internode development. High rooting rates (40 %) were only obtained in the first morphotype shoots exposed for 5 min to 2.4 µM IBA and then transferred to OM medium without growth regulators. Plants were acclimatized in pots containing a mixture of peat and Perlite (3:2) in greenhouse with progressive reduction of relative humidity. A flow cytometric screening for major ploidy changes revealed no differences among the morphotypes and between them and the mother plant. Also the nuclear DNA content of this species was estimated for the first time using flow cytometry (2C = 24.71 pg).

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Section: Introductionmentioning

confidence: 99%

Micropropagation of Juniperus phoenicea from adult plant explants and analysis of ploidy stability using flow cytometry

Loureiro¹,

Capelo²,

Brito³

et al. 2007

Biol. plant.

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“…Therefore, in vitro biotechnological methods such as micropropagation and somatic embryogenesis are being used with commercial tree species (Zhu et al 1997;Aderkas and Bonga 2000;Mauri et al 2001). However, differentiation, organogenesis and somatic embryogenesis are difficult to achieve from the mature phase of trees and remain a major problem for a number of crop species (Gomez and Segura 1996;Sangwan et al 1997). On the other hand, the improvement of fruit trees by biotechnological methods requires the development of efficient regeneration systems (Caboni 2000).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis in cell suspension cultures of olive Olea europaea (L.) ‘Chetoui’

Trabelsi

Naija

Elloumi³

et al. 2010

Acta Physiol Plant

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Somatic embryogenesis of olive Olea europaea (L.) 'Chetoui' was studied using cell suspension cultures initiated from mature leaf-derived calli. Calli were developed on half-strength MS medium supplemented with 10 lM NAA and 2.25 lM 2i-P in the dark. Different combinations of three plant growth regulators (2,4-D, NAA and zeatin) were tested to determine cell proliferation and somatic embryogenesis induction and differentiation. Embryogenic suspension cultures were established in olive-modified medium for embryogenesis (OMe) containing 2.5 lM 2,4-D and 2.5 lM zeatin. Pre-globular and globular embryos were induced from mature olive tissue in liquid medium. In addition, the nitrogen form as inorganic (reduced; (NH 4 ) 2 SO 4 or oxidized; KNO 3 ) and organic (CH) was used separately or in combination to improve the cell growth and proliferation. The most effective growth rate and cell proliferation were obtained with the medium containing inorganic and organic nitrogen forms.

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“…However, reports from the Cupressaceae family are scarce. Up to now there is only one report showing that it is possible to produce somatic embryos in Juniperus (J. oxycedrus), however, no mature somatic embryos nor plants were obtained (Gomez and Segura 1996). We have chosen common juniper, Juniperus communis, as a model species for the genus Juniperus for studying possibilities and limitations with somatic embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

Embryogenic cell lines of Juniperus communis; easy establishment and embryo maturation, limited germination

Helmersson

Arnold

2008

Plant Cell Tiss Organ Cult

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Several coniferous species belonging to the Pinaceae family can be propagated via somatic embryogenesis, while species belonging to the Cupressaceae family cannot. The aim of this study was to identify possibilities and limitations with somatic embryogenesis in Cupressaceae. Juniperus communis was chosen as model species. We show that a high initiation frequency of embryogenic cell lines can be established from intact megagametophytes at the time when intensive cleavage polyembryogeny takes place. The embryogenic cell lines proliferate fast on medium lacking plant growth regulators. Early somatic embryos develop after transfer to medium with decreased content of nitrogen and calcium. The early embryos mature after exposure to abscisic acid. Mature cotyledonary embryos germinate after partial desiccation. A high proportion, over 40%, of the germinating embryos retain the embryogenic potential in the basal part, resulting in development of new embryogenic tissue.

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Morphogenesis in leaf and single-cell cultures of mature Juniperus oxycedrus

Cited by 14 publications

References 25 publications

Micropropagation of Juniperus phoenicea from adult plant explants and analysis of ploidy stability using flow cytometry

Micropropagation of Juniperus phoenicea from adult plant explants and analysis of ploidy stability using flow cytometry

Somatic embryogenesis in cell suspension cultures of olive Olea europaea (L.) ‘Chetoui’

Embryogenic cell lines of Juniperus communis; easy establishment and embryo maturation, limited germination

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