Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas

Sujatha, M.; Mukta, N.

doi:10.1007/bf00048191

Cited by 122 publications

(100 citation statements)

References 9 publications

(9 reference statements)

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“…This represents regeneration efficiency higher than that reported by Li et al (2008) under similar experimental conditions. Although we cannot deduce to what extent genotypic difference may have led to these discrepancies, similar result which placed the percentage at 50%, was reported by other authors (Sujatha and Mukta, 1996) where the same kind of explants were incubated in a media supplemented with a 1:2 concentration ratio of BA and IBA. In our case, up to 84.35% of the explants attained their initial stage of shoot formation five weeks after incubation although not all of them later developed into shoots.…”

Section: Shoot Formationsupporting

confidence: 78%

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Analysis of organogenic competence of cotyledons of Jatropha curcas and their in vitro histological behavior

Nogueira¹,

Soares²,

Ibrahim³

et al. 2011

Afr. J. Biotechnol.

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Using cut pieces cotyledons from germinating zygotic embryos of Jatropha curcas, we monitored the series of anatomical events leading to the generation of shoot through organogenesis by histological analysis. 14 days old cotyledons that were pre-cultured in a half strength Murashige and Skoog (MS) media supplemented with 100 mg/L myoinositol and 10 mg/L thiamine HCl, were cultured in organogenic competence induction media (CIM) comprising of MS salts with 1.5 mg/L benzyl adenine (BA) and 0.05 mg/L indole-3-butyric acid (IBA) and incubated for the induction of organogenic competence. Following their sequential transfer to shoot induction media (containing MS + 1.5 mg/L BA, 0.05 mg/L IBA and 0.5 mg/L GA 3 ); shoot elongation media (containing MS + 0.3 mg/L BA) and rooting media (containing half strength MS + IBA at different concentrations), we selected individual explants and subjected them to histological analysis in order to study the morphological changes occurring during organogenesis. Our findings show that to induce organogenesis using cut pieces of cotyledons, these tissues must be harvested at least by the 14th day following germination. Optimal organogenic competence was attained after 21 days incubation period in the dark where most of the explants showed evidence of protruding shoots surrounded by calli with various morphological features. Up to 53.91% of the total explants cultured in this study produced well defined shoots with each explant producing an average number of 1.5 shoots bearing two to eight leaves. We recorded the highest percentage of root formation, which stood at 27.50% when the shoots were cultured in a rooting media containing 0.3 mg/L IBA. Histological analysis of the different events occurring during the process of organogenesis suggest that the protuberances arising from parenchymatous cells and perhaps bundle sheath forming meristematic centres acquiring organogenic competence have a multicellular origin, indicating that the regeneration process takes place through direct organogenesis.

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Section: Shoot Formationsupporting

confidence: 78%

“…This underscores the importance of the acquisition of organogenic competence induced by the combining IBA and BA within a concentration range (Sujatha and Mukta, 1996).…”

Section: Acquisition Of Organogenic Competencementioning

confidence: 69%

Analysis of organogenic competence of cotyledons of Jatropha curcas and their in vitro histological behavior

Nogueira¹,

Soares²,

Ibrahim³

et al. 2011

Afr. J. Biotechnol.

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“…To meet the large-scale demand and ensure easy supply of this elite material, there is a need to develop mass multiplication techniques. Attempts have been made to regenerate toxic variety of J. curcas (Sujatha and Mukta 1996;Rajore and Batra 2007;Jha et al 2007;Deore and Johnson 2008;Misra et al 2010;Kumar and Reddy 2010;Kumar et al 2010a). However, there is very few reports on non-toxic variety of J. curcas where regeneration was obtained from leaf with the presence of intermediary callus (Sujatha et al 2005) and petiole (Kumar et al 2010b(Kumar et al , 2011.…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants

Kumar

Anand

Reddy

2011

J. Plant Biochem. Biotechnol.

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“…Young leaves have been proved to be callus in other plant species, such callus potential has been reported to vary from species to species and often differs in varieties of same species (Vasil, 1982). Similar observations have been reported in Jatropha integerrima (Sujatha and Dhingra, 1993); Jatropha curcus ( Sujatha and Mukta, 1996); Solanum tuberosum (Jayasree et al, 2001); Solanum torvum (Jaseela and Nair, 2004); Rauwolfia micrantha (Vishwanath et al, 1997); Rauwolfia serpentina (Nishi Koshta et al, and Anitha et al, 2002).Combinations of BAP and NAA have been used for regeneration by many investigators. …”

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confidence: 53%

In vitro Plant Regeneration from Leaf Explants of Artemisia vulgaris L. – A Medicinal Herb

Borzabad¹,

Sudarshana²,

Niranjan³

2010

MAS

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A reliable protocol for callus induction and regeneration were developed for leaf explants of Artemisia vulgaris L. Percent of callus induction and regeneration were higher in the young leaves. MS medium containing 1.0 mgl -1 BAP and 3.0 mgl -1 NAA is the optimum concentration for induction of callus. So produced callus was subcultured on Murashige -Skoog (MS) medium with 1.0 mgl -1 6-benzylaminopurine (BAP), 3.0 mgl -1 gibberellic acid (GA 3 ) produced the highest mean number of shoots (35.85±0.81) per explant. Half strength of MS was found to be the best for rooting, however, addition of IAA (0.5-1.0 mgl -1 ) was found essential to induce longer roots. More than 84% of the rooted plants were established in polycups after hardening.Keywords: Callus induction, Plant regeneration, Leaf explants, Artemisia vulgaris L IntroductionArtemisia vulgaris Linn, an important perennial medicinal herb belongs to the family Asteraceae. The plant is aromatic, shrubby 0.6-2.4m high and pubescent. The plant has a hot, sharp and pungent taste. It is considered to be a valuable stomachic, deobstruent and antispasmodic. The expressed juice is used in diseases of children. The leaves and shoot tips are administered in nervous and spasmodic affections connected with debility, in asthma and diseases of the brain. It shows antispasmodic and anthelmintic activity (Kirtiker and Basu, 1935).Leaf explants have been employed for regeneration by many researchers observed in Centaurreum erythraea (Baresova et al., 1985); Jatropha integerrima (Sujatha and Dhingra,1993); Solanum pseudocapsicanum (Baburaj and Gunasekaran, 1994); Guizotia abssinica (Jadimath, 1998); Jatropha curcus (Sardana et al. ,2000). The present study describes an optimized regeneration system in Artemisia vulgaris callus derived from leaf explant cultures on a variety of medium composition. Materials and methods Plant materialLeaf explants from 6-month-old mature plant of Artemisia vulgaris L. were used to initiate in vitro cultures. The leaf segments were washed with 5% (w/v) bavistin, 10% (w/v) antibiotic and 5% (w/v) Tween 20 by continuous shaking for 20 min followed by washing in tap water, then rinsed three to five times with double distilled water. Callus inductionTo induce callus, the sterile leaf explants were inoculated on MS Medium, containing BAP (0.5-1.0 mgl Shoot induction and elongationShoots were induced by transferring the leaf calli on MS medium containing different concentrations and combinations of BAP (0.5-1.0 mgl -1 ) and GA 3 (0.5-3.0 mgl -1 ). Elongation of shoots were also observed on the same medium. Rooting and AcclimatizationMicroshoots were excised from the parent cultures and transferred onto half strength MS medium supplemented with different concentrations and combinations of IBA, IAA and NAA for root induction. The rooted shoots were gently removed from the culture vessels, washed under running tap water and transferred to polycups containing sand:soil:vermiculite (1:1:1) in the greenhouse conditions for acclimatization. Results an...

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Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas

Cited by 122 publications

References 9 publications

Analysis of organogenic competence of cotyledons of Jatropha curcas and their in vitro histological behavior

Analysis of organogenic competence of cotyledons of Jatropha curcas and their in vitro histological behavior

Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants

In vitro Plant Regeneration from Leaf Explants of Artemisia vulgaris L. – A Medicinal Herb

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