Morphogenesis and Pathogenesis Regulation of Candida Albicans by Probiotic Bacterium – Pediococcus Acidilactici

Rezaie, Sassan; Zareshahrabadi, Zahra; Zomorodian, Kamiar; Pakshir, Keyvan; Mehrabani, Davood; Nouraei, Hasti; Motamedi, Marjan; Hashemi, Seyed Jamal; Ranjbaran, Alireza; Nazemi, Ladan; Ahmadikia, Kazem

doi:10.15414/jmbfs.2020.10.1.5-11

Cited by 11 publications

(9 citation statements)

References 40 publications

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“…Also, sometimes dematiaceous yeasts could cause superficial mycosis 8 . The clinical manifestations of superficial and cutaneous Candida infection vary, such as oral thrush, oropharyngeal candidiasis, vaginal candidiasis, cutaneous candidiasis, paronychia, and onychomycosis, and chronic mucocutaneous candidiasis 9,10 …”

Section: Introductionmentioning

confidence: 99%

Prevalence of superficial‐cutaneous fungal infections in Shiraz, Iran: A five‐year retrospective study (2015–2019)

Khodadadi

Zomorodian

Nouraei

et al. 2021

Clinical Laboratory Analysis

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Background Superficial and cutaneous fungal infections are common in tropical areas. The aim of this study was to provide a basic database of superficial and cutaneous mycoses and the most common etiological agents among patients. Methods Between 2015 and 2019, a total of 1807 patients suspected of superficial and cutaneous mycosis referring to the mycology laboratory of Shiraz medical school, Fars, Iran were evaluated. Specimens were taken from the patients’ affected area, and clinical samples were examined by direct microscopy and culture. The epidemiological profile of the patients was collected. Results A total of 750 patients were confirmed with mycoses. Positive samples totaled 750 cases consisting of the nail (373/49.7%), skin (323/43%), head (47/6.26%), and mucosal membrane (4/0.5%). The yeasts group included 304 Candida spp. (70.3%), 123 Malassezia spp. (28.47%), and 5 Rhodotorula spp. (1.1%). The filamentous fungi were distributed as 34.8% dermatophytes and 7.5% non‐dermatophyte. The clinical types of dermatophytosis were tinea unguium (110/261), tinea capitis (50/261), tinea pedis (48/261), tinea corporis (37/261), and tinea cruris (16/261). Non‐dermatophyte molds included A. flavus 17, A. niger 4, Aspergillus spp. 15, Penicillium. 10, Fusarium 6, Mucor 2, Stemphylium 1, and Alternaria 1. Conclusion This study provides useful data for the study trends of superficial and cutaneous fungal infections in a specific area. The mycological data confirmed higher incidence of candidiasis (mainly onychomycosis) and dermatophytosis in patients affected by fungal pathogens, which helped to better understand the epidemiological aspects of these mycoses.

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Section: Introductionmentioning

confidence: 99%

Prevalence of superficial‐cutaneous fungal infections in Shiraz, Iran: A five‐year retrospective study (2015–2019)

Khodadadi

Zomorodian

Nouraei

et al. 2021

Clinical Laboratory Analysis

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“…The growth in each well was compared with that of the growth control well. MICs were visually determined and defined as the lowest concentration of NPs that completely inhibited the growth of standard strains of bacteria and C. albicans [ 8 ].…”

Section: Methodsmentioning

confidence: 99%

“…Microbial biofilms are 3D structures that have gained the ability to resist antimicrobials and immune cell challenges. It is noteworthy that environmental conditions under the denture as well as the structure of materials supported this microbial growth and played a key role in denture-related stomatitis [ 7 , 8 ]. Denture stomatitis is a common disease in denture wearers (15–70%) which mainly contributes to Candida spp.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial and Antibiofilm Activities of Copper Oxide Nanoparticles within Soft Denture Liners against Oral Pathogens

Ansarifard

Zareshahrabadi

Sarafraz

et al. 2021

Bioinorganic Chemistry and Applications

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Objectives. Soft denture liners provide a favorable environment for adhesion and colonization of microorganisms. This in vitro study aimed to examine the efficacy of different concentrations of copper oxide nanoparticles (CuO NPs) incorporation into soft denture liner on the biofilm formation of the microbial species. Methods. Field Emission Scanning Electron Microscopy (FESEM) images from NPs were recorded. Antifungal susceptibility testing of CuO NPs against five standard strains of Candida albicans (CBS 10261, 1905, 1912, 1949, 2730), Streptococcus mutans (ATCC35668), Streptococcus sobrinus (ATCC27607), and Streptococcus salivarius (ATCC9222) was performed by the broth microdilution method with the Clinical and Laboratory Standards Institute reference method. The biofilm inhibition percentages of CuO NPs on the soft denture liners were determined by XTT assay. Results. The characterization of CuO NPs by scanning electron microscope (SEM) analyses confirmed the synthesis of NPs with appropriate structure and size with a mean diameter of 18.3 ± 9.1 nm. The CuO NPs successfully inhibited the growth of the tested standard strains of C. albicans and Streptococcus spp. at concentrations ranging from 64 to 128 µg mL−1. Indeed, incorporation of CuO NPs at a concentration of 500 µg mL−1 into the soft denture liners exhibited a significant activity (75%) in inhibition of C. albicans. biofilm formation in a dose-dependent manner. The biofilm formation of C. albicans in the presence of CuO NPs was lower than Streptococcus spp. in comparison with the control group ( p < 0.05 ). Conclusion. Incorporation of CuO NPs significantly decreased the colonization and plaque formation of the oral pathogens, especially C. albicans accumulation. These NPs may be useful as a promising agent for the antimicrobial management of soft denture liner materials.

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“…The microbial cells were harvested and washed twice in sterile phosphate-buffered saline (PBS) (0.8% ( w / v ), NaCl (Merck); 0.02% ( w / v ), KH 2 PO 4 (Merck); 0.31% ( w / v ), Na 2 HPO 4 +12H 2 O (Merck); and 0.02% ( w / v ), KCl (PanReac); pH 7.4). Then, the bacterial and fungal strains were resuspended in MHB and RPMI-1640, respectively, and cell densities were adjusted to 1.0 × 10 6 cells/mL after counting with a hemocytometer [ 22 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

Characterization and Physical and Biological Properties of Tissue Conditioner Incorporated with Carum copticum L.

Hejazi

Zareshahrabadi

Ashayeri

et al. 2021

BioMed Research International

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Aim. One of the main problems in dentistry is the injury caused by the long-term application of an ill-fitting denture. The existence of multiple microorganisms along with the susceptibility of the tissue conditioners to colonize them can lead to denture stomatitis. This study is aimed at developing a tissue conditioner incorporated with Carum copticum L. (C. copticum L.) for the effective treatment of these injuries. Materials and Methods. The Carum copticum L. essential oil composition was determined by gas chromatography-mass (GC-mass) spectrometry. The antimicrobial activity of the essential oil against the standard strains of bacterial and fungal species was determined by broth microdilution methods as suggested by the Clinical and Laboratory Standards Institute (CLSI). The physical and chemical properties of the prepared tissue conditioner were investigated by viscoelasticity, FTIR assays, and the release study performed. Furthermore, the antibiofilm activity of the Carum copticum L. essential oil-loaded tissue conditioner was evaluated by using the XTT reduction assay and scanning electron microscopy (SEM). Results. The main component of the essential oil is thymol, which possesses high antimicrobial activity. The broth microdilution assay showed that the essential oil has broad activity as the minimum inhibitory concentration was in the range of 32-128 μg mL-1. The viscoelasticity test showed that the essential oil significantly diminished the viscoelastic modulus on the first day. The FTIR test showed that Carum copticum L. essential oil was preserved as an independent component in the tissue conditioner. The release study showed that the essential oil was released in 3 days following a sustained release and with an ultimate cumulative release of 81%. Finally, the Carum copticum L. essential oil exhibited significant activity in the inhibition of microbial biofilm formation in a dose-dependent manner. Indeed, the lowest and highest amounts of biofilm formation on the tissue conditioner disks are exhibited in the Streptococcus salivarius and Candida albicans by up to 22.4% and 71.4% at the 64 μg mL-1 concentration of C. copticum L. with a statistically significant difference ( P < 0.05 ). Conclusion. The obtained results showed that the Carum copticum L. essential oil-loaded tissue conditioner possessed suitable physical, biological, and release properties for use as a novel treatment for denture stomatitis.

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Morphogenesis and Pathogenesis Regulation of Candida Albicans by Probiotic Bacterium – Pediococcus Acidilactici

Cited by 11 publications

References 40 publications

Prevalence of superficial‐cutaneous fungal infections in Shiraz, Iran: A five‐year retrospective study (2015–2019)

Prevalence of superficial‐cutaneous fungal infections in Shiraz, Iran: A five‐year retrospective study (2015–2019)

Evaluation of Antimicrobial and Antibiofilm Activities of Copper Oxide Nanoparticles within Soft Denture Liners against Oral Pathogens

Characterization and Physical and Biological Properties of Tissue Conditioner Incorporated with Carum copticum L.

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