Morphofunctional Characterization of Cooled Sperm With Different Extenders to Use in Equine-Assisted Reproduction

Florez-Rodriguez, S. A.; Arruda, Rubens Paes de; Alves, Maíra Bianchi Rodrigues; Affonso, Frank José; Carvalho, Henrique F.; Lemes, Kleber Menegon; Andrade, André Furugen Cesar de; Celeghini, Eneiva Carla Carvalho

doi:10.1016/j.jevs.2014.04.007

Cited by 12 publications

(9 citation statements)

References 27 publications

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“…Florez‐Rodriguez et al. (), after the cooling process, and Sardoy et al. () observed a significant increase in DNA fragmentation after cryopreservation with the TB stain.…”

Section: Discussionmentioning

confidence: 93%

“…This technique is based on the susceptibility of the free phosphate groups of DNA and sperm protamines in the unbound binding to basic dyes (Shamsi, Imam, & Dada, ). This means that in situations with several free phosphate groups more ruddy (in blue) will be the DNA (Florez‐Rodriguez et al., ). The TB is a fast, low‐cost and efficient method, which can be incorporated into the routine seminal analysis as a screening fertility test (Erenpreisa et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…In fact, the TB stain is commonly used in human medicine for sperm DNA analysis (Erenpreisa et al., ). On the other hand, there are few studies in domestic and laboratory animals, such as horses (Florez‐Rodriguez et al., ), rabbit (Beletti & Mello, ), llama ( Lama gama ) (Carretero et al., ), mouse (Krzanowska, ) and recently in dogs (Flores et al., ). To the best of our knowledge, no studies were performed in cats.…”

Section: Introductionmentioning

confidence: 99%

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A fast, low‐cost and efficient method for the diagnosis of sperm DNA fragmentation in several species

Rui

Angrimani

Bicudo

et al. 2017

Reprod Domestic Animals

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Sperm DNA fragmentation is a condition that interferes directly in the reproductive efficiency. Currently, there are several methods for assessing the sperm DNA integrity, such as Alkaline Comet, TUNEL and Sperm Chromatin Structure Assay. However, many of these techniques are laborious and require high-precision equipment. Thus, the development of new techniques can optimize the evaluation of sperm DNA damage. Therefore, the aim of this study was to standardize the toluidine blue (TB) stain technique for the analysis of DNA fragmentation of dog, cat, bull, stallion and ram spermatozoa. For this purpose, we used six animals of each specie (n = 30), in reproductive age. Sperm was collected by different methods according to the particularities of each species, and such samples were divided into two aliquots: a sperm sample was kept at 5°C (considered as intact sperm DNA), and the remaining samples were submitted to the induction of DNA fragmentation by exposure to ultraviolet light for 4 hr. Samples were then mixed with the intact sample to obtain known and progressive proportions of sperm with fragmented DNA (0%, 25%, 50%, 75% and 100%). Semen smears were performed and subjected to staining with TB. Blue-stained spermatozoa were considered to have DNA fragmentation. We observed high linear regression coefficients between the expected proportion of damaged DNA and the results of TB for dog, cat, ram, bull and stallion samples. In conclusion, TB stain was considered a fast and effective technique for the study of spermatozoa DNA in several species.

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“…Florez‐Rodriguez et al. (), after the cooling process, and Sardoy et al. () observed a significant increase in DNA fragmentation after cryopreservation with the TB stain.…”

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A fast, low‐cost and efficient method for the diagnosis of sperm DNA fragmentation in several species

Rui

Angrimani

Bicudo

et al. 2017

Reprod Domestic Animals

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“…Sperm protection by milk contains active components such as casein micelles, which interact with membrane plasma proteins and protect against the removal of phospholipids and cholesterol from the plasma membrane [22]. Despite advances in the development of extenders, skim milk-based extender currently presents performance as good as other more complex and modern extenders [23].…”

Section: Effect Of Frozen-thawed Equine Sperm Dilution Using Skim Milmentioning

confidence: 99%

Addition of Pentoxifylline to Skim Milk–Based Extender on Frozen-Thawed Equine Sperm

Tsunoda

Arruda

Serrano-Recalde

et al. 2015

Journal of Equine Veterinary Science

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“…Although techniques and equipment are increasingly advanced, sperm characteristics may be altered during the cryopreservation process, and there may be variations between lots. Moreover, during the evaluation, the semen is analyzed based on the average, taking into account that the ejaculate is a homogeneous sample, but it is already known that the ejaculate has heterogeneous subpopulations, both in morphology and kinetics, and may be related to the change in individual performance of the bull, affecting the final results, and the application of additional techniques for semen evaluation helps to improve quality control (FLOREZ-RODRIGUEZ et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Factors which interfere in the results of the Fixed-Time Artificial Insemination in beef cattle

Ferreira¹,

Murta²,

Menezes³

et al. 2021

Rev Agr Acad

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The objective of this study was to evaluate the effect of the animal category, the cyclicity, the CIDR reuse, the body condition score (BCS), the inseminator and the bull on the fertility rates of bovine submitted to a FTAI protocol. It was observed that pluriparous demonstrated a higher fertility. The group With Corpus Luteum obtained a higher conception rate. The CIDR of 4th use reduced (P <0.05) the chance of conception. The chances of conception in cows of the Low BCS group were lower (P <0.05), than the cows with intermediate and high BCS. The inseminator and the bull exerted influence on the fertility. Therefore, all factors are important in FTAI programs.

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Morphofunctional Characterization of Cooled Sperm With Different Extenders to Use in Equine-Assisted Reproduction

Cited by 12 publications

References 27 publications

A fast, low‐cost and efficient method for the diagnosis of sperm DNA fragmentation in several species

A fast, low‐cost and efficient method for the diagnosis of sperm DNA fragmentation in several species

Addition of Pentoxifylline to Skim Milk–Based Extender on Frozen-Thawed Equine Sperm

Factors which interfere in the results of the Fixed-Time Artificial Insemination in beef cattle

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