Morphofunctional Changes in Colon after Cold Stress in Male C57BL/6 Mice Susceptible and Tolerant to Hypoxia

Золотова, Н.А.; Джалилова, Д. Ш.; Цветков, И.С.; Косырева, А. М.; Ponomarenko, E A; Диатроптова, М. А.; Михайлова, Л. П.; Мхитаров, В А; Makarova, Olga

doi:10.1007/s10517-021-05259-4

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…Cold or humid environments are considered extreme conditions in both nature and human society, and they have been shown to adversely affect intestinal function and induce physiological and immune responses in animals (Scharf et al, 2019;Huang B. et al, 2022). There is a growing body of evidence indicating that cold or rainy conditions can amplify the incidence of diarrhea (Xu et al, 2013;Thongprachum et al, 2015;Thiam et al, 2017;Huang et al, 2021;Zolotova et al, 2021). This phenomenon is particularly observed in livestock, where it is referred to as "cold Wu et al 10.3389/fmicb.2023.1288430 Frontiers in Microbiology 02 frontiersin.org stress diarrhea" (Gou et al, 2021;Sun et al, 2022;Zhou et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Alterations in intestinal microbiota and enzyme activities under cold-humid stress: implications for diarrhea in cold-dampness trapped spleen syndrome

Wu,

Deng,

Liu

et al. 2023

Front. Microbiol.

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IntroductionCold and humid environments alter the intestinal microbiota, and the role of the intestinal microbiota in the development of diarrhea associated with cold-dampness trapped spleen syndrome in Chinese medicine is unclear.MethodsThe 30 mice were randomly divided into normal and model groups, with the model group being exposed to cold and humid environmental stresses for 7 days. Then, mouse intestinal contents were collected and analyzed their intestinal microbiota and digestive enzymes.ResultsOur findings revealed significant increases in sucrase and lactase activities, as well as microbial activity, in the model group (p < 0.05). β-diversity analysis highlighted distinct intestinal microbiota compositions between the two groups. Specifically, the experimental group showed a unique dominance of the genera and strains Clostridium sensu stricto 1 and Clostridium sp. ND2. LEfSe analysis identified Helicobacter, Roseburia, and Eubacterium plexicaudatum ASF492 as differentially abundant species in them model group. Network analysis demonstrated that rare bacterial species mostly governed the microbial interactions, exhibiting increased mutual promotion. On the other hand, abundant species like Lactobacillus johnsonii and Lactobacillus reuteri showed mutual inhibitory relationships.DiscussionIn summary, exposure to cold and humid conditions led to increased intestinal enzyme activities and a shift in microbial composition, favoring the growth of rare bacterial species. These changes suggest that rare bacteria in the intestinal microbiota play a critical role in the pathology of diarrhea associated with cold-dampness trapped spleen syndrome, revealing unique survival strategies among bacterial populations under stressful conditions.

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Section: Introductionmentioning

confidence: 99%

Alterations in intestinal microbiota and enzyme activities under cold-humid stress: implications for diarrhea in cold-dampness trapped spleen syndrome

Wu,

Deng,

Liu

et al. 2023

Front. Microbiol.

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Cold stress changes the composition and function of microbiota in the content and mucosa of the ileum and colon in piglets

Zhang,

Li,

Wang

et al. 2024

Anim. Prod. Sci.

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Context Cold stress is a significant factor that contributes to the imbalance of energy in piglets during their early life. Recent studies have shown that gut microbiota plays a crucial role in maintaining energy homeostasis under cold-stress conditions. Aims This study aims to investigate the effects of cold stress on the microbiota and expression pathways in the colon and ileum of weaned piglets, providing new insights and methods for helping piglets resist cold stress. Methods In total, 10 piglets in the cold-stress group (n = 5) and room-temperature group (n = 5) were sacrificed. Intestinal contents and mucosa samples were collected for 16S rRNA analysis. Key results The results showed that cold stress increased the observed features and chao1 index in the colonic mucosa. The beta diversity of ileum, colon and ileum mucosa was significantly changed. Under cold stress, the relative abundance of Acholeplasma, Proteiniphilum, and Olsenella increased in the contents of the ileum and colon. The relative abundance of Ruminococcaceae sp., Butyricicoccus, and Lachnospiraceae FCS020 increased in the mucosa of the colon. Sphingomonas, Helicobacter, Cutibacterium, and Bradyrhizobium were significantly increased in the mucosa of the ileum. In predicted functions, after cold stress, the purine metabolism and degradation increased in the content and mucosa of the ileum and mucosa of the colon. The fat biosynthesis pathway increased in the content of the colon. Complex carbohydrate degradation increased in the mucosa of both. Conclusions These findings suggest that cold stress has a significant impact on the species richness, composition, and predicted functions of the microbiota in the ileum and colon of piglets, with these effects varying depending on the location within the gut. Implications Therefore, we can help piglets resist cold stress by modifying the structure of gut microbiota through the addition of probiotics or adjusting the composition of their diet.

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RNA-seq analysis of small intestine transcriptional changes induced by starvation stress in piglets

Ma,

Guo,

Ding

et al. 2023

Animal Biotechnology

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Morphofunctional Changes in Colon after Cold Stress in Male C57BL/6 Mice Susceptible and Tolerant to Hypoxia

Cited by 3 publications

References 14 publications

Alterations in intestinal microbiota and enzyme activities under cold-humid stress: implications for diarrhea in cold-dampness trapped spleen syndrome

Alterations in intestinal microbiota and enzyme activities under cold-humid stress: implications for diarrhea in cold-dampness trapped spleen syndrome

Cold stress changes the composition and function of microbiota in the content and mucosa of the ileum and colon in piglets

RNA-seq analysis of small intestine transcriptional changes induced by starvation stress in piglets

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