Morphoanatomical and biochemical factors associated with rice resistance to the South American rice water weevil, Oryzophagus oryzae (Coleoptera: Curculionidae)

Abstract

The rice water weevil, Oryzophagus oryzae (Coleoptera: Curculionidae), is an economically important pest of flooded rice paddies throughout South America, and species with similar life histories are present in many rice-producing regions globally (collectively referred to here as RWWs). Plant resistance is a key strategy for management of RWWs; however, the mechanisms responsible for rice resistance to RWWs are poorly understood. We investigated morphoanatomical and biochemical plant traits potentially involved in rice resistance to O. oryzae. Resistance-associated traits were characterized in two cultivars, ‘Dawn’ (resistant) and ‘BRS Pampa CL’ (‘Pamp’ = susceptible), which were selected from among six cultivars on 2-year field screenings. Anatomical and morphological traits of leaf tissues from ‘Pamp’ and ‘Dawn’ were similar, which perhaps explains the lack of antixenosis during host plant selection. However, significant antibiosis effects were found. The activities of antioxidant enzymes involved in plant defense, as well the content of hydroxycinnamic and hydroxybenzoic acids derivatives and lignin, were higher in roots of ‘Dawn’ than in ‘Pamp’, over the period of larval infestation in the field. Additionally, ‘Dawn’ exhibited a root sclerenchyma arranged in three layers of lignified cells, which differed from the arrangement of cells in ‘Pamp’, regardless of larval infestation. Our results provide the first evidence for specific resistance-related traits associated with mortality and malnutrition of RWWs in rice.