Morpho-Physiological and Stress-Related Gene Expression of Rice Varieties in Response to Salinity Stress at Early Vegetative Stage

Shakri, Tasneem; Che-Othman, Muhamad Hafiz; Isa, Nurulhikma Md; Sukiran, Noor Liyana; Zainal, Zamri

doi:10.3390/agriculture12050638

Cited by 10 publications

(6 citation statements)

References 70 publications

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“…Rice is a very important cereal crop that feeds half of the world's population and is particularly sensitive to salt stress [ 38 ]. There have been many studies on salt-tolerant transgenic rice, most of which were based on research into homologous species and gene families to identify the genes that change the salt response of rice [ 39 – 41 ]. Prior to the current study, the OsDUF6 gene had no known function.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of OsDUF6 increases salt stress tolerance in rice

Ma,

Zhang,

2024

BMC Plant Biol

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Background Soil salinity is one of the primary environmental stresses faced in rice production. When plants are exposed to salt stress, a series of cellular balances will be disrupted. Dufulin is an immune-induced antiviral agent used in plants. The DUF gene family influences plant response to abiotic stress, and the functional role of OsDUF6(ABA98726.1) in rice response to salt stress is being investigated here. Results Based on the transcriptome analysis of Dufulin treatment in inducing salt tolerance in rice, we selected the OsDUF6 protein located on the cell membrane and studied its molecular function by overexpressing OsDUF6. Salt-induced decreases in root, stem, and leaf length and increased leaf yellowing rate and Na+ concentration in the wild-type plant were mitigated in the overexpressed lines. OsDUF6 overexpression increased the enzymatic antioxidant activities of superoxide dismutase, peroxidase, catalase, and phenylalanine ammonia-lyase. OsDUF6 also played a positive role in Na+ transport as reflected by the increased growth of a salt-sensitive yeast mutant complemented with OsDUF6 in the presence of salt stress. In addition, Reverse transcription quantitative PCR analysis confirmed that the overexpression of OsDUF6 significantly changed the expression level of other genes related to growth and stress tolerance. Conclusions Combined with previously published data, our results supported the observation that OsDUF6 is an important functional factor in Dufulin-induced promotion of salt stress tolerance in rice.

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Section: Discussionmentioning

confidence: 99%

Overexpression of OsDUF6 increases salt stress tolerance in rice

Ma,

Zhang,

2024

BMC Plant Biol

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“…Rice is a very important cereal crop that feeds half of the world's population and is particularly sensitive to salt stress [31]. There have been many studies on salt-tolerant transgenic rice, most of which were based on research into homologous species and gene families to identify the genes that change the salt response of rice [32][33][34]. Prior to the current study, the OsDUF6 gene had no known function.…”

Section: Discussionmentioning

confidence: 99%

Dufulin activates OsDUF6 protein against salt stress in rice plant

Ma,

zhang,

2024

Preprint

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Background Dufulin is a chemical immune activator in rice plant. Soil salinity is one of the main environmental stresses in rice production. When plants are exposed to salt stress, a range of cellular equilibria will be disrupted. Previous studies have shown that Dufulin has a positive effect on salt tolerance in rice. Results In this study, we studied the mechanism of Dufulin in response to salt stress. Based on the transcriptome analysis of Dufulin in the process of salt tolerance in rice, we selected the OsDUF6 protein located on the cell membrane and studied its molecular function by overexpression of OsDUF6. The results showed that the salt-induced decreases in root, stem, and leaf length and increased leaf yellowing rate and Na+ concentration in the wild-type plant were improved in the overexpressed lines, and increased the enzyme activity of the SOD, POD, CAT and PAL. OsDUF6 played a positive role in Na + transport by comparing the growth of the salt-sensitive yeast mutant complemented with OsDUF6. In addition, RT-qPCR analysis confirmed that the overexpression of OsDUF6 significantly changed the expression level of genes related to growth and stress tolerance. Conclusions Combined with previously published data, our results supported that OsDUF6 is an important functional factor of Dufulin to promote salt stress resistance in rice and plays a role in promoting salt tolerance in rice.

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“…This activity will cause an impediment to vital processes like the photosynthesis of plants by declining photosynthetic efficiency as well as lead to incomplete inflorescence (Boyes et al, 2001;. The reduction of relative chlorophyll content in atsap2 was recorded and this is possibly associated with the loss-of-function of the gene and contributes to the rapid oxidative damage in the chloroplast and lipid membrane peroxidation of thylakoids (Kaur et al, 2019;Sharma et al, 2020b;Shakri et al, 2022). However, unlike OsSAP8, the overexpression of the gene results in a maintained level of relative chlorophyll content in response to both stresses.…”

Section: Discussionmentioning

confidence: 99%

Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis

Mohd Roszelin,

Mohd Hazbir,

Jumali

et al. 2023

MAB

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Environmental stress can hinder the growth and development of crops, thereby reducing productivity. Plants can adapt to changing environments through various morpho-physiological changes, transcriptome regulation, signaling, translational and post-translational modifications. Stress Associated Proteins (SAPs) have been shown to play a crucial role in plant adaptation to biotic and abiotic stressors. They are encoded by a family of genes that produce a zinc finger protein with A20 and/or AN1 domains at either their N or C-terminal ends. Therefore, this study focused on understanding the role of the Oryza sativa SAP gene family (OsSAPs) in response to drought and salinity stress. In-silico analysis revealed that most of the OsSAP family members were upregulated by stress; two highly inducible OsSAP genes were also upregulated in response to stress under a rice-specific background. To study gene function, an Arabidopsis transformation system was employed using three genotypes: Col-0 (wild type), overexpressed transgenic OsSAP8, and atsap2 T-DNA knockout mutant. Arabidopsis AtSAP2 gene, which is homologous to rice OsSAP8, was used as a comparison to the loss of function mutation in Arabidopsis. Morphophysiological analysis showed that the atsap2 mutant displayed a sensitive phenotype to drought and salinity stress through low relative chlorophyll content and delayed inflorescence development and flowering as compared to Col-0 and transgenic OsSAP8. This suggests that the abolished atsap2 gene may contribute to reduced stress tolerancein plants. In contrast, transgenic OsSAP8 overexpression demonstrated tolerance to drought and salinity stress by maintaining relative chlorophyll content under both stress conditions, indirectly reflecting sustained photosynthetic machinery and stable photosynthetic rate. Further investigation, such as measuring the photosynthesis rate, is required to establish the correlation between chlorophyll data and photosynthesis activity.

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Morpho-Physiological and Stress-Related Gene Expression of Rice Varieties in Response to Salinity Stress at Early Vegetative Stage

Cited by 10 publications

References 70 publications

Overexpression of OsDUF6 increases salt stress tolerance in rice

Overexpression of OsDUF6 increases salt stress tolerance in rice

Dufulin activates OsDUF6 protein against salt stress in rice plant

Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis

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