Morpho-physiological and genetic characteristics of a salt-tolerant mutant line in soybean (Glycine max L.)

Moon, Chang Yeok; Kang, Byeong Hee; Kim, Woon Ji; Chowdhury, Sreeparna; Kang, Sehee; Lee, Jeong‐Dong; Kwon, Soon‐Jae; Kim, Han-Yong; Lee, Hyeon-Seok; Ha, Bo‐Keun

doi:10.1007/s00122-023-04408-9

Cited by 4 publications

(4 citation statements)

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“…In this study, we conducted a transcriptome analysis to identify DEGs in the roots and leaves of the soybean cultivar ‘Kwangan’ and the salt-tolerant mutant ‘KA-1285’, which was induced by gamma-ray irradiation, in response to salt stress. The major salt tolerance QTL of the KA-1285 was mapped to ‘ GmSalt3 ’ region on chromosome 3 [ 27 ]. This mutant exhibited reduced accumulation of Cl − in leaves and increased accumulation of Cl − in roots compared to the original cultivar.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we used two soybeans, ‘Kwangan’ and ‘KA-1285’, which have different tolerance levels for salt stress, for transcriptome profiling. According to the evaluation of salt tolerance in a previous study [ 27 ], KA-1285 (9.2 mg/g) showed a decrease in Cl − accumulation in its leaves when compared to Kwangan (18.2 mg/g), following a two-week exposure to 200 mM NaCl. However, peculiarly, the accumulation of Cl − in the roots of KA-1285 (12.8 mg/g) exhibited a slight increase compared to Kwangan (10.3 mg/g).…”

Section: Methodsmentioning

confidence: 99%

“…To validate the DEGs, we selected some DEGs exhibiting genomic variations in the coding regions of KA-1285 compared with Kwangan, which were detected through a previous re-sequencing analysis [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

“…Previously, a salt-tolerant mutant called KA-1285 was generated from the salt-sensitive soybean cultivar Kwangan using gamma-ray irradiation [ 27 ]. The generated KA-1285 exhibited a salt-tolerance level similar to that of S-100 [ 28 ] and PI483463 [ 29 ], both of which were previously reported to be salt-resistant, based on visual evaluation under conditions of salt stress.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Profiling of a Soybean Mutant with Salt Tolerance Induced by Gamma-ray Irradiation

Kang,

Chowdhury,

Kang

et al. 2024

Plants

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Salt stress is a significant abiotic stress that reduces crop yield and quality globally. In this study, we utilized RNA sequencing (RNA-Seq) to identify differentially expressed genes (DEGs) in response to salt stress induced by gamma-ray irradiation in a salt-tolerant soybean mutant. The total RNA library samples were obtained from the salt-sensitive soybean cultivar Kwangan and the salt-tolerant mutant KA-1285. Samples were taken at three time points (0, 24, and 72 h) from two tissues (leaves and roots) under 200 mM NaCl. A total of 967,719,358 clean reads were generated using the Illumina NovaSeq 6000 platform, and 94.48% of these reads were mapped to 56,044 gene models of the soybean reference genome (Glycine_max_Wm82.a2.v1). The DEGs with expression values were compared at each time point within each tissue between the two soybeans. As a result, 296 DEGs were identified in the leaves, while 170 DEGs were identified in the roots. In the case of the leaves, eight DEGs were related to the phenylpropanoid biosynthesis pathway; however, in the roots, Glyma.03G171700 within GmSalt3, a major QTL associated with salt tolerance in soybean plants, was differentially expressed. Overall, these differences may explain the mechanisms through which mutants exhibit enhanced tolerance to salt stress, and they may provide a basic understanding of salt tolerance in soybean plants.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%