Morpho‐palynological study of Cyperaceae from wetlands of Azad Jammu and Kashmir using SEM and LM

Abstract

In this study 12 species of Cyperaceae have been studied for quantitative and qualitative observation of pollen grains through Light and scanning electron microscopy. Pollens of 12 species of Cyperaceae from different wetlands of Azad Jammu and Kashmir were collected. Morphological characters of pollen grains were then investigated under the Light and Scanning electron microscope. Two pollen types have been observed apolar and heteropolar. Shape of pollens was prolate (4 spp), sub-spheroidal (7 spp), and oblate (1 spp). Variation observed in exine sculpturing granular (4 spp), reticulate (1 spp), areolate-punctate (3 spp), and psilate (2 spp). Polar to equatorial ratio and fertility percentage of the pollens were also studied. Based on these micromorphlogical characters of pollens taxonomic keys have been made for the accurate identification of the members of Cyperaceae. The characteristics studied in present research work are very much valuable taxonomically and phytochemically for the identification of species of family Cyperaceae. Light microscope (LM) and Scanning electron microscope (SEM) were used for pollen observation, which play vital role in the taxonomical identification of species and provide sufficient information for taxonomist.

Synthesis, characterization and use of iron oxide nano particles for antibacterial activity

“…SEM played a key role to explore the size and morphology of the NPs because the biological behavior of the NPs strongly depends on size and shape (Laurent et al, ). SEM has proven to be very valuable for the observation of the surface morphology of material, mainly due to improved depth of field and high resolution, which is not available under light microscopy (Butt et al, ; Esfandani‐Bozchaloyi & Zaman, ; Shah, Ahmad, Zafar, Malik, et al, ; Shah, Ahmad, Zafar, Razzaq, et al, ; Ullah, Zafar, Ahmad, Shah, et al, ; Ullah, Zafar, Ahmad, Dilbar, et al, ; Ullah, Zafar, Amhad, Sultana, et al, ). In the last few years the SEM has made a massive impact on diverse branches of biological research (Ashfaq et al, ; Bahadur et al, ; Shah, Celik et al, ; Sufyan et al, ; Ullah, Papini, et al, ; Ullah, Zaman, et al, ).…”

Palyno‐anatomical studies of monocot taxa and its taxonomic implications using light and scanning electron microscopy

“…Exine often reticulate, microreticulate scabrate, and psilate are taxonomically significant features examined under SEM to identify monocot taxa at microlevel. Similarly, Ashfaq et al (), Bahadur et al (), Butt et al (), Kosenko (), Sufyan et al (), Ullah, Zafar, Ahmad, Dilbar, et al () investigated pollen of various taxa, using LM and SEM techniques and reported that the pollen morphological characteristics were of important characters for the taxonomic identifications of species and genera in different plant families. The LM and SEM study show variation between the species with special emphasis on exine ornamentation, which has a significant role to support the taxonomy of problematic and closely related taxa (Carrijo et al, ; Lopes, Andreata, Cartaxo‐Pinto, Trovó, & Gonçalves‐Esteves, ; Nurit‐Silva & De Fátima Agra, ).…”

Palyno‐morphological investigations of halophytic taxa of Amaranthaceae through SEM from Salt range of Northern Punjab, Pakistan

“…(Lamiaceae) using SEM and LM have been reported (Akçin & Camili, ). Butt et al () also utilized LM and SEM for morpho‐palynological study of Cyperaceae. The use of LM and SEM is of special interest for the taxonomist to examine various taxonomic features (Khan, Ahmad, Zafar, & Ullah, ).…”