Morpho-histological study on shoot bud regeneration in cotyledon cultures of pepper (Capsicum annuum)

Mezghani, Najla; Jemmali, Ahmed; Elloumi, Nedhra; Gargouri-Bouzid, Radhia; Kintzios, Spyridon

doi:10.2478/s11756-007-0146-9

Cited by 18 publications

(5 citation statements)

References 26 publications

(21 reference statements)

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“…As for the interaction, a higher mean number of shoots /explant was recorded (7.26) in cotyledon at 4.0 mg/l BA combined with 0.5 mg/L IAA followed by (6.96) at 5 mg/L BA combined with 0.5 mg/L IAA without significant differences . leaf explant recorded the highest mean number of shoots per explants (5.4) at 4.0 mg/L BA combined with 0.5 mg/L IAA (Table1).This result was agreed with Mezghani et al, (2007) they found that the maximum shoot bud induction of Capsicum annuum using cotyledons as explant cultured on BA in combination with IAA compared with those of control and other treatments. Auxins are using to increase the activating enzyme that break down starch and has the ability to move the active leading to increased proliferation of organogenesis (Taiz and Zeiger2009).The role of auxin in multiplications has been described by Majda and Robert(2018).…”

Section: Resultssupporting

confidence: 86%

An Efficient Protocol for Direct Organogenesis of Pepper ( Capsicum annuum) التکوین العضوى المباشر فى نبات الفلفل

Aboshama

Amer

Hussein

2020

Journal of Plant Production

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A simple and efficient plantlet regeneration protocol was established for Pepper (Capsicum annum L.) achieved through direct organogenesis from Leaves and cotyledons explants. Leaves were taken from 21 day-old in vitro plantlets and and cultured on Murashige and Skoog (1962) MS medium supplemented with different growth regulators. Thidiazuran (TDZ)or Benzyl adenine (BA) with Indole 3-acetic acid (IAA) containing medium was tested. Prolific direct adventitious shoot regeneration occurred on most of the tested growth regulators. The best response in terms of frequency of shoots number per explant (10.5) was obtained from cotyledon cultured on 1.0 mg/L TDZ in combination with 0.5 mg/L IAA. To elongate the shoots it were cultured on MS medium supplemented with various concentrations of gibberellic acid (GA3) in combination with 0.25 mg/L Kinetin (KIN). The highest shoot length (2.2 cm) was achieved on MS medium supplemented with 0.5 mg/L GA3 and 0.25 mg/L KIN. Regenerated shoots were excised to root. The highest number of root/shoot was obtained with quarter MS salt medium supplemented with 1.0 mg/L indole-3-butyric acid (IBA) with high length of root (4.60). plantlets were finally transferred to mixture of peatmoss, vermiculite and sand at equal volume 1:1:1.The plantlets showed 80 % survival after 30 days during acclimatization and transplanting.

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Section: Resultssupporting

confidence: 86%

An Efficient Protocol for Direct Organogenesis of Pepper ( Capsicum annuum) التکوین العضوى المباشر فى نبات الفلفل

Aboshama

Amer

Hussein

2020

Journal of Plant Production

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“…This response occurred especially in genotype AVA-8. Mezghani et al (2007) interpreted the low number of elongated plants found in their work as a consequence of the induction of teratological protuberances, structures that are frequently associated with fasciated and degenerative meristems. Whereas the leaves of shoots developed from hypocotyls had a good appearance ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Improvement of regeneration in pepper: a recalcitrant species

Gammoudi

Pedro

Ferchichi

et al. 2017

In Vitro Cell.Dev.Biol.-Plant

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Organogenesis is influenced by factors like genotype, type of explant, culture medium components, and incubation conditions. The influence of ethylene, which can be produced in the culture process, can also be a liming factor in recalcitrant species like pepper. In this work, bud induction was achieved from cotyledons and hypocotyls -from eight pepper cultivars -on Murashige and Skoog (MS) medium supplemented with 22.2 µM 6-benzyladenine (6BA) and 5.7 µM indole-3-acetic acid (IAA), in media with or without silver nitrate (SN) (58.9 µM), a suppressor of ethylene action. In the SN-supplemented medium, the frequencies of explants with buds and with callus formation were lower in both kinds of explant, but higher numbers of developed shoots were isolated from explants cultured on SN. Bud elongation was better in medium with gibberellic acid (GA3) (2.9 µM) than in medium free of growth regulators or supplemented with 1aminocyclopropane-1-carboxylic acid (ACC) at 34.5 µM. However, isolation of shoots was difficult and few plants were recovered. The effect of adding SN following bud induction (at 7 d) and that of dark incubation (the first 7 d of culture) was also assessed in order to improve the previous results. When SN was added after bud induction, similar percentages of bud induction were found for cotyledons (average frequency 89.4% without SN and 94.4% with SN) whereas they doubled in hypocotyls (50 % without SN and 87.7% with SN). In addition, in both kinds of explant, the number of developed plants able to be transferred to soil (developed and rooted) was greatly increased by SN. Dark incubation does not seem to improve organogenesis in pepper, and hypocotyl explants clearly represent a better explant choice -with respect to cotyledonary explants -for the pepper cultivars assayed. REGENERATION IN PEPPER3

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“…A strong influence of culture conditions and genotypes has also been demonstrated (Venkataiah et al, 2003). By using cotyledon explants (Agrawal et al, 1989;Mathew, 2002;Venkataiah et al, 2006) and by applying the most common regeneration protocols for hot pepper (Mathew, 2002;Venkataiah et al, 2001), Mezghani et al (2007) produced shoot buds for a hot pepper genotype (cv. Baklouti).…”

Section: Discussionmentioning

confidence: 99%

Improvement of In Vitro Proliferation and Elongation of Habanero Pepper Shoots (Capsicum chinense Jacq.) by Temporary Immersion

Bello-Bello¹,

Canto‐Flick²,

Balam-Uc³

et al. 2010

horts

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This article describes the performance of nodal segments from Habanero pepper (Capsicum. chinense) during shoot induction and elongation under different semisolid and liquid culture conditions with various degrees of ventilation in which they were exposed to different levels of immersion and growth regulators. The ethylene content in non-ventilated containers, the age of the explant donor plants as well as the effect of thidiazuron and paclobutrazol on shoot induction and of gibberellic acid and AgNO 3 on shoot elongation were also evaluated. A temporary immersion bioreactor (BioMINTä) was used for the multiplication and elongation of isolated shoots with very good results. We report an efficient protocol for the in vitro propagation of Habanero pepper that produces plants with a high survival rate when transplanted to soil.

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Morpho-histological study on shoot bud regeneration in cotyledon cultures of pepper (Capsicum annuum)

Cited by 18 publications

References 26 publications

An Efficient Protocol for Direct Organogenesis of Pepper ( Capsicum annuum) التکوین العضوى المباشر فى نبات الفلفل

An Efficient Protocol for Direct Organogenesis of Pepper ( Capsicum annuum) التکوین العضوى المباشر فى نبات الفلفل

Improvement of regeneration in pepper: a recalcitrant species

Improvement of In Vitro Proliferation and Elongation of Habanero Pepper Shoots (Capsicum chinense Jacq.) by Temporary Immersion

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