Morpho-Functional Characterization of Human Mesenchymal Stem Cells from Umbilical Cord Blood for Potential Uses in Regenerative Medicine

Barachini, Serena; Trombi, Luisa; Danti, Serena; D’Alessandro, Delfo; Battolla, Barbara; Legitimo, Annalisa; Nesti, Claudia; Mucci, I; D’Acunto, Mario; Cascone, Maria Grazia; Lazzeri, Luigi; Mattii, Letizia; Consolini, Rita; Petrini, Mario

doi:10.1089/scd.2008.0017

Cited by 46 publications

(39 citation statements)

References 41 publications

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“…52 The morphology and differentiation potential of MSCs are dependent upon their source, the culture conditions (supplements, seeding density, number of passages, culture time) and the topographic localization within each colony. 49,53,54 The characteristics of the 3 D scaffolds, such as their chemical composition, topography, and roughness, have been also recognized as significant factors in affecting the cells' behavior. The heterogeneity and morpho-functional variability of MSCs and their microenvironment may explain some of the conflicting data in different research works.…”

Section: Discussionmentioning

confidence: 99%

Study of the involvement of allogeneic MSCs in bone formation using the model of transgenic mice

Kuznetsova

Prodanets

Rodimova

et al. 2016

Cell Adhesion & Migration

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Mesenchymal stem cells (MSCs) are thought to be the most attractive type of cells for bone repair. However, much still remains unknown about MSCs and needs to be clarified before this treatment can be widely applied in the clinical practice. The purpose of this study was to establish the involvement of allogeneic MSCs in the bone formation in vivo, using a model of transgenic mice and genetically labeled cells. Polylactide scaffolds with hydroxyapatite obtained by surface selective laser sintering were used. The scaffolds were sterilized and individually seeded with MSCs from the bone marrow of 5-week-old GFP(C) transgenic C57/Bl6 or GFP(¡)C57/Bl6 mice. 4-mm-diameter critical-size defects were created on the calvarial bone of mice using a dental bur. Immediately after the generation of the cranial bone defects, the scaffolds with or without seeded cells were implanted into the injury sites. The cranial bones were harvested at either 6 or 12 weeks after the implantation. GFP(C) transgenic mice having scaffolds with unlabeled MSCs were used for the observation of the host cell migration into the scaffold. GFP(¡) mice having scaffolds with GFP(C) MSCs were used to assess the functioning of the seeded MSCs. The obtained data demonstrated that allogeneic MSCs were found on the scaffolds 6 and 12 weeks post-implantation. By week 12, a newly formed bone tissue from the seeded cells was observed, without an osteogenic predifferentiation. The host cells did not appear, and the control scaffolds without seeded cells remained empty. Besides, a possibility of vessel formation from seeded MSCs was shown, without a preliminary cell cultivation under controlled conditions.

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Section: Discussionmentioning

confidence: 99%

Study of the involvement of allogeneic MSCs in bone formation using the model of transgenic mice

Kuznetsova

Prodanets

Rodimova

et al. 2016

Cell Adhesion & Migration

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“…Mononuclear cells derived from human umbilical cord blood and obtained by a negative immunoselection technique exhibited either osteoclast-like or mesenchymal-like phenotypes [47] . However, these cells were able to produce homogeneous populations of MSCs displaying a fibroblast-like morphology while expressing mesenchyme-related antigens and showing differentiation capability for osteoblastic and early chondroblastic lineages.…”

Section: Therapeutic Application Of Umbilical Cord Mscs For Joint Patmentioning

confidence: 99%

“…However, these cells were able to produce homogeneous populations of MSCs displaying a fibroblast-like morphology while expressing mesenchyme-related antigens and showing differentiation capability for osteoblastic and early chondroblastic lineages. This study by Barachini et al [47] is one of the few investigating human umbilical cord blood-derived MSC growth and differentiation on three-dimensional scaffolds. The potential applications in regenerative medicine and tissue engineering of umbilical cord blood-derived MSCs were illustrated by their ability to grow on biodegradable microfiber meshes and their capability to differentiate into mature osteoblasts when cultured inside human plasma clots; this suggests their potential applications in orthopedic surgery.…”

Section: Therapeutic Application Of Umbilical Cord Mscs For Joint Patmentioning

confidence: 99%

Umbilical cord as a mesenchymal stem cell source for treating joint pathologies

Arufe

Fuente²,

Fuentes³

et al. 2011

WJO

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Articular cartilage disorders and injuries often result in life-long chronic pain and compromised quality of life. Regrettably, the regeneration of articular cartilage is a continuing challenge for biomedical research. One of the most promising therapeutic approaches is cellbased tissue engineering, which provides a healthy population of cells to the injured site but requires differentiated chondrocytes from an uninjured site. The use of healthy chondrocytes has been found to have limitations. A promising alternative cell population is mesenchymal stem cells (MSCs), known to possess excellent proliferation potential and proven capability for differentiation into chondrocytes. The "immunosuppressive" property of human MSCs makes them an important candidate for allogeneic cell therapy. The use of allogeneic MSCs to repair large defects may prove to be an alternative to current autologous and allogeneic tissue-grafting procedures. An allogeneic cell-based approach would enable MSCs to be isolated from any donor, expanded and cryopreserved in allogeneic MSC banks, providing a readily available source of progenitors for cell replacement therapy. These possibilities have spawned the current exponential growth in stem cell research in pharmaceutical and biotechnology communities. Our objective in this review is to summarize the knowledge about MSCs from umbilical cord stroma and focus mainly on their applications for joint pathologies.

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“…Umbilical cord blood also has a low contaminate rate of malignant cells and low immunogenicity [2,3].…”

Section: Introductionmentioning

confidence: 99%

“…Several research groups have demonstrated that mesenchymal stem cells (MSCs), the precursor cells of stromal cells [6][7][8], also exist in hUCB [2,[9][10][11][12][13][14]. Human umbilical cord blood-derived mesenchymal stem cells (hUCBDMSCs) obtained from colony-forming unit fibroblasts (CFU-F) display a homogeneous morphology and express some CD molecules that are characteristic of bone marrow-derived MSCs [12].…”

Section: Introductionmentioning

confidence: 99%

Human Umbilical Cord Blood-Derived Stromal Cells Are Superior to Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Inducing Myeloid Lineage Differentiation In Vitro

Sun

Zhang²,

Chen³

et al. 2012

Stem Cells and Development

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Stromal cells and mesenchymal stem cells (MSCs), 2 important cell populations within the hematopoietic microenvironment, may play an important role in the development of hematopoietic stem/progenitor cells. We have successfully cultured human umbilical cord blood-derived stromal cells (hUCBDSCs). It has been demonstrated that MSCs also exist in hUCB. However, we have not found any reports on the distinct characteristics of hUCBDSCs and human umbilical cord blood-derived mesenchymal stem cells (hUCBDMSCs). In this study, hUCBDSCs and hUCBDMSCs were isolated from the cord blood of full-term infants using the same density gradient centrifugation and cultured in the appropriate medium. Some biological characteristics and hematopoietic supportive functions were compared in vitro. hUCBDSCs were distinct from hUCBDMSCs in morphology, proliferation, cell cycle, passage, immunophenotype, and the capacity for classical tri-lineage differentiation. Finally, quantitative real-time polymerase chain reaction analysis revealed that granulocyte colony-stimulating factor (G-CSF) gene expression was higher in hUCBDSCs than that in hUCBDMSCs. Enzyme-linked immunosorbent assay revealed that the secretion of G-CSF, thrombopoietin (TPO), and granulocyte macrophage colony-stimulating factor (GM-CSF) by hUCBDSCs was higher than that by hUCBDMSCs. After coculture, the granulocyte/macrophage colony-forming units (CFU-GM) of hematopoietic cells from the hUCBDSC feeder layer was more than that from the hUCBDMSC feeder layer. Flow cytometry was used to detect CD34+ hematopoietic stem/progenitor cell committed differentiation during 14 days of coculture; the results demonstrated that CD14 and CD33 expression in hUCBDSCs was significantly higher than their expression in hUCBDMSCs. This observation was also true for the granulocyte lineage marker, CD15. This marker was expressed beginning at day 7 in hUCBDSCs. It was expressed earlier and at a higher level in hUCBDSCs compared with hUCBDMSCs. In conclusion, hUCBDSCs are different from hUCBDMSCs. hUCBDSCs are superior to hUCBDMSCs in supporting hematopoiesis stem/progenitor cells differentiation into myeloid lineage cells at an early stage in vitro.

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Morpho-Functional Characterization of Human Mesenchymal Stem Cells from Umbilical Cord Blood for Potential Uses in Regenerative Medicine

Cited by 46 publications

References 41 publications

Study of the involvement of allogeneic MSCs in bone formation using the model of transgenic mice

Study of the involvement of allogeneic MSCs in bone formation using the model of transgenic mice

Umbilical cord as a mesenchymal stem cell source for treating joint pathologies

Human Umbilical Cord Blood-Derived Stromal Cells Are Superior to Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Inducing Myeloid Lineage Differentiation In Vitro

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