Morpho-functional characterization of cultured pigment cells fromRana esculenta L. Liver

Pintucci, Giuseppe; Manzionna, Mariano; Maida, Isabel; Boffi, Monica; Boffoli, Domenico; Gallone, Anna; Cicero, R.

doi:10.1007/bf02624421

Cited by 11 publications

(14 citation statements)

References 21 publications

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“…Similar pigmented macrophage structures have been described in amphibians and reptiles (Scalia, Geremia, Corsaro, Santoro, Sciuto & Sichel 1988; Pintucci, Manzionna, Maida, Boffi, Gallone, Boffoli & Cicero 1990; Zuastic, Jiménez‐Cervantes, Garcia‐Boron & Ferrer 1998). Sichel, Scalia, Mondio & Corsaro (1997) have carried out extensive studies on the extracutaneous, melanin‐producing, Kupffer cells of the liver of amphibia.…”

Section: Phylogenysupporting

confidence: 77%

Melano‐macrophage centres and their role in fish pathology

Agius

Roberts

2003

Journal of Fish Diseases

702

537

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Melano-macrophage centres, also known as macrophage aggregates, are distinctive groupings of pigment-containing cells within the tissues of heterothermic vertebrates. In fish they are normally located in the stroma of the haemopoietic tissue of the spleen and the kidney, although in amphibians and reptiles, and some fish, they are also found in the liver. They may also develop in association with chronic inflammatory lesions elsewhere in the body and during ovarian atresia. In higher teleosts, they often exist as complex discrete centres, containing lymphocytes and macrophages, and may be primitive analogues of the germinal centres of lymph nodes. Melano-macrophage centres usually contain a variety of pigments, including melanins, and these increase in range and volume in older fish or in the presence of cachectic disease. Melano-macrophage centres act as focal depositories for resistant intracellular bacteria, from which chronic infections may develop. Iron capture and storage in haemolytic diseases appears to be a primary function, but antigen trapping and presentation to lymphocytes, sequestration of products of cellular degradation and potentially toxic tissue materials, such as melanins, free radicals and catabolic breakdown products are among other functions that have been ascribed. Recent work suggests that they are a site of primary melanogenesis rather than mere storage. Melano-macrophage centres increase in size or frequency in conditions of environmental stress and have been suggested as reliable biomarkers for water quality in terms of both deoxygenation and iatragenic chemical pollution.

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Section: Phylogenysupporting

confidence: 77%

Melano‐macrophage centres and their role in fish pathology

Agius

Roberts

2003

Journal of Fish Diseases

702

537

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“…The sinusoidal cells were isolated from the frog liver by digestion with 0.2% (wt/vol) pronase E (Sigma Chemical Co., St. Louis, MO), after liver perfusion (17). The sinusoidal cells were isolated from the frog liver by digestion with 0.2% (wt/vol) pronase E (Sigma Chemical Co., St. Louis, MO), after liver perfusion (17).…”

Section: Methodsmentioning

confidence: 99%

“…Besides having a phagocytic capacity, frog liver pigment cells in culture have been demonstrated to be able to generate superoxide anions after stimulation with a phorbol ester, like the cells of phagocytic lineage (17).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural and functional study of the liver pigment cells from Rana esculenta L.

Guida

Maida

Gallone

et al. 1998

In Vitro Cell.Dev.Biol.-Animal

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A study of the liver pigment cells of Rana esculenta L. has been performed on both liver in toto and cells in culture. Ultrastructural and cytochemical analyses showed a close relationship between this visceral pigment cell system and the cells of hepatic macrophage lineage. Like the latter, the liver pigment cells present phagocytic activity, in the sinusoids and in vitro, and give a positive response to tests for peroxidase and lipase. The liver pigment cells are isolated, together with the Kupffer cells, from the sinusoidal cell fraction of the liver. In culture, they maintain their melanogenetic ability, demonstrated by the presence of dopaoxidase activity in the soluble, membranous, and melanosome fractions. Analysis of the cultures showed that as culture time increased, so did melanosome dopaoxidase activity, the number of pigmented fields, and the level of pigmentation of the cells. The values of dopaoxidase activity of the pigment cells in culture show the same seasonal oscillations as the system in toto, indicating that the cells maintain an internal clock, at least in the first 72 h of culture. There is evidence that the pigment cells are macrophages which can express a melanogenetic function. Our results and other experimental data provide a basis for hypothesizing that the pigment cells in Rana esculenta L. liver may derive from, or have a common origin with, the Kupffer cells.

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“…The experiments were performed according to the method of Guida et al (1998) with minor modifications. The livers from Rana, after perfusion, were excised, minced and subjected to digestion (Pintucci et al 1990), then filtered through a sterile nylon gauze. The filtrate supernatant, after being allowed to settle for 10 min, was centrifuged at 600 g for 5 min.…”

Section: Isolation and Culture Of Kupffer Cells From Rana Livermentioning

confidence: 99%

Characterisation of Kupffer cells in some Amphibia

et al. 2000

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A study on the Kupffer cells (KCs) of Amphibia was undertaken in order to compare these cells with those of endothermic animals. Liver tissue and isolated and cultured KCs were studied by light microscopy and by transmission and scanning electron microscopy. We have shown that amphibian KCs can be divided into 2 principal types : ' small ' and ' large '. Both cell types possess the distinctive KC morphology. They show nonspecific esterase activity, weak endogenous peroxidase activity in the nuclear envelope and in the rough endoplasmic reticulum, and the ability to engulf naturally present cell debris or experimentally administered zymosan or latex particles. The principal difference between the small and the large cells consists in the substantial quantity of inclusion bodies that exist only in the latter. We conclude that amphibian KCs, apart from their ability to build melanosomes and synthesise melanins, are very similar to mammalian KCs.

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Morpho-functional characterization of cultured pigment cells fromRana esculenta L. Liver

Cited by 11 publications

References 21 publications

Melano‐macrophage centres and their role in fish pathology

Melano‐macrophage centres and their role in fish pathology

Ultrastructural and functional study of the liver pigment cells from Rana esculenta L.

Characterisation of Kupffer cells in some Amphibia

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