Morpho-elasticity of human pluripotent stem cell cysts

Ackermann, Joseph; Cohen, Philippe J.R.; Alessandri, Kévin; Leonard, Andrea; Nassoy, Pierre; Joanny, Jean‐François; Amar, Martine Ben

doi:10.1016/j.jmps.2022.104778

Cited by 7 publications

(7 citation statements)

References 42 publications

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“…5(e)). This effect can be described using a generic morphoelastic model by assuming that cyst growth is anisotropic 26 . However, deeper biological investigation, which is beyond the scope of the present paper, would be required to decipher the underlying mechanism.…”

Section: Resultsmentioning

confidence: 99%

zIncubascope: long-term quantitative imaging of multi-cellular assemblies inside an incubator

Jana,

Mekhlieri,

Boyreau

et al. 2024

Preprint

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Recent advances in bioengineering have made it possible to develop increasingly complex biological systems to recapitulate organ functions as closely as possible in vitro. Monitoring the assembly and growth of multi-cellular aggregates, micro-tissues or organoids and extracting quantitative information is a crucial but challenging task required to decipher the underlying morphogenetic mechanisms. We present here an imaging platform designed to be accommodated inside an incubator which provides high-throughput monitoring of cell assemblies over days and weeks. We exemplify the capabilities of our system by investigating human induced pluripotent stem cells (hiPSCs) enclosed in spherical capsules, hiPSCs in tubular capsules and yeast cells in spherical capsules. Combined with a customized pipeline of image analysis, our solution provides insight into the impact of confinement on the morphogenesis of these self-organized systems.

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Section: Resultsmentioning

confidence: 99%

zIncubascope: long-term quantitative imaging of multi-cellular assemblies inside an incubator

Jana,

Mekhlieri,

Boyreau

et al. 2024

Preprint

Self Cite

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“…Among other examples, we can mention the case of proliferating cancer-activated fibroblasts [23, 12], spindle cell sarcomas [24, 25] or MDCK cells [10], but also the final steps of wound healing performed by myo-fibroblasts [26, 27] or fibrosis occurring in pathologies and around implants [28, 29, 30]. Tissue growth often follows tissue anisotropy, and stresses resulting from this anisotropic growth can have a feedback effect on the growth itself [8]. Therefore, anisotropic growth must be taken into account when modelling cancer growth [31].…”

Section: Growth Of Nematic Cellsmentioning

confidence: 99%

“…In this case the epithelium is called pseudostratified. Their growth has been shown to be highly anisotropic [8]. Since most human cancers originate from epithelial tissues, it is crucial to pay attention to this growth category.…”

Section: Growth Of Polar Cellsmentioning

confidence: 99%

“…We show here that this polarity, observed at the scale of a single cell, determines the shape of a multicellular epithelium. A typical example concerns the cysts of stem cells in the human epiblast phase in fetal life or in experiments in vitro with human pluripotent stem cells [8] as shown in Fig. 1(a).…”

Section: Introductionmentioning

confidence: 99%

“…Polarity and nematic properties are also important players in cell proliferation, as they are often crucial for understanding single cell or collective cell behaviours [6,7]. Cells in Growing cyst of polar cells (from [8]). Polarity induces an anisotropy in growth, which in turn creates stresses that have a feedback effect on the growth.…”

Section: Introductionmentioning

confidence: 99%

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Onsager’s variational principle in proliferating biological tissues, in presence of activity and anisotropy

Ackermann,

Ben Amar

2023

Preprint

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A hallmark of biological cells is their ability to proliferate and of tissues their ability to grow. This is common in morphogenesis and embryogenesis but also in pathological conditions such as tumour growth. To consider these tissues from a physical point of view, it is necessary to derive fundamental relationships, in particular for velocities and density components, taking into account growth terms, chemical factors and the symmetry of cells and tissues. The aim is then to develop a consistent coarse-grained approach to these complex systems, which exhibit proliferation, disorder, anisotropy and activity at small scales. To this end, Onsager's variational principle allows the systematic derivation of flux-force relations in systems out of equilibrium and the principle of the extremum of dissipation, first formulated by Rayleigh and revisited by Onsager, finally leads to a consistent formulation for a continuous approach in terms of a coupled set of partial differential equations. Considering the growth and death rates as fluxes, as well as the chemical reactions driving the cellular activities, we derive the momentum equations based on a leading order physical expansion. Furthermore, we illustrate the different interactions for systems with nematic or polar order at small scales, and numerically solve the resulting system of partial differential equations in relevant biophysical growth examples. To conclude, we show that Onsager's variational principle is useful for systematically exploring the different scenarios in proliferating systems, and how morphogenesis depends on these interactions.

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Statistical physics of active matter, cell division and cell aggregation

Joanny,

Indekeu

2023

Physica A: Statistical Mechanics and its Applications

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Morpho-elasticity of human pluripotent stem cell cysts

Cited by 7 publications

References 42 publications

zIncubascope: long-term quantitative imaging of multi-cellular assemblies inside an incubator

zIncubascope: long-term quantitative imaging of multi-cellular assemblies inside an incubator

Onsager’s variational principle in proliferating biological tissues, in presence of activity and anisotropy

Statistical physics of active matter, cell division and cell aggregation

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