Morpho-densitometric traits for quinoa (Chenopodium quinoa Willd.) seed phenotyping by two X-ray micro-CT scanning approaches

Gargiulo, Laura; Grimberg, Åsa; Repo‐Carrasco‐Valencia, Ritva; Carlsson, Anders S.; Mele, Giacomo

doi:10.1016/j.jcs.2019.102829

Cited by 27 publications

(23 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These components are mainly stored in the embryo of the seed [ 6 ], which suggests that the embryo portion of the QU5 seeds is small, especially if compared to the other quinoa samples. The study by Gargiulo et al [ 40 ] stated that the embryo of the quinoa seed has a higher density compared to the perisperm. The smaller embryo to perisperm ratio explains why sample QU5 had a lower test weight.…”

Section: Resultsmentioning

confidence: 99%

Comparison of the Chemical and Technological Characteristics of Wholemeal Flours Obtained from Amaranth (Amaranthus sp.), Quinoa (Chenopodium quinoa) and Buckwheat (Fagopyrum sp.) Seeds

Bock

Daelemans

Selis

et al. 2021

Foods

View full text Add to dashboard Cite

A sound fundamental knowledge of the seed and flour characteristics of pseudocereals is crucial to be able to promote their industrial use. As a first step towards a more efficient and successful application, this study focuses on the seed characteristics, chemical composition and technological properties of commercially available pseudocereals (amaranth, quinoa, buckwheat). The levels of starch, fat, dietary fiber and minerals were comparable for amaranth and quinoa seeds but the protein content is higher in amaranth. Due to the high amount of starch, buckwheat seeds are characterised by the lowest amounts of fat, dietary fibre and minerals. Its protein content ranged between that of amaranth and quinoa. Buckwheat seeds were larger but easily reduced in size. The lipid fraction of the pseudocereals mostly contained unsaturated fatty acids, with the highest prevalence of linoleic and oleic acid. Palmitic acid is the most abundant unsaturated fatty acid. Moreover, high levels of P, K and Mg were found in these pseudocereals. The highest phenolic content was found in buckwheat. Amaranth WMF (wholemeal flour) had a high swelling power but low shear stability. The pasting profile strongly varied among the different quinoa WMFs. Buckwheat WMFs showed high shear stability and rate of retrogradation.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparison of the Chemical and Technological Characteristics of Wholemeal Flours Obtained from Amaranth (Amaranthus sp.), Quinoa (Chenopodium quinoa) and Buckwheat (Fagopyrum sp.) Seeds

Bock

Daelemans

Selis

et al. 2021

Foods

View full text Add to dashboard Cite

show abstract

“…To characterize nutrient composition during seed development, we cultivated three quinoa genotypes under controlled environment and sampled the seeds at different developmental stages. The protein content of these seeds at maturity was previously shown to be different for these genotypes ( Gargiulo et al, 2019 ). The three selected quinoa genotypes, i.e., Titicaca, Pasankalla rosada (henceforth called Pasankalla) and Regalona Baer (henceforth called Regalona), are from different ecoregions and, therefore, have different day-length sensitivities.…”

Section: Resultsmentioning

confidence: 97%

“…By studying genotypes with contrasting seed quality, we aim to identify genetic factors that regulate protein content that can become plant breeding targets for developing new, improved varieties with increased protein content in the grain. In a recently published study, we compared three quinoa genotypes of diverse origins that were identified to have contrasting seed protein content, and, through X-ray micro-tomography, a positive correlation was found between protein content and embryo proportion ( Gargiulo et al, 2019 ). This is not surprising if we consider that the majority of the protein in quinoa seeds is, in fact, deposited in the embryo tissues ( Burrieza et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Regulation of Quinoa Seed Quality: Identification of Novel Candidate Genetic Markers for Increased Protein Content

et al. 2022

Self Cite

View full text Add to dashboard Cite

Quinoa (Chenopodium quinoa Willd.) is a crop that has great potential for increased cultivation in diverse climate regions. The seed protein quality obtained from this crop is high concerning the requirements to meet human nutritional needs, but the seed protein content is relatively low if compared to crops such as grain legumes. Increased seed protein content is desirable for increasing the economic viability of this crop in order for it to be used as a protein crop. In this study, we characterized three genotypes of quinoa with different levels of seed protein content. By performing RNA sequencing of developing seeds, we determined the genotype differences in gene expression and identified genetic polymorphisms that could be associated with increased protein content. Storage nutrient analyses of seeds of three quinoa genotypes (Titicaca, Pasankalla, and Regalona) from different ecoregions grown under controlled climate conditions showed that Pasankalla had the highest protein content (20%) and the lowest starch content (46%). Our seed transcriptome analyses revealed highly differentially expressed transcripts (DETs) in Pasankalla as compared to the other genotypes. These DETs encoded functions in sugar transport, starch and protein synthesis, genes regulating embryo size, and seed transcription factors. We selected 60 genes that encode functions in the central carbon metabolism and transcription factors as potential targets for the development of high-precision markers. Genetic polymorphisms, such as single nucleotide polymorphisms (SNPs) and base insertions and deletions (InDels), were found in 19 of the 60 selected genes, which can be further evaluated for the development of genetic markers for high seed protein content in quinoa. Increased cultivation of quinoa can contribute to a more diversified agriculture and support the plant protein diet shift. The identification of quinoa genotypes with contrasting seed quality can help establish a model system that can be used for the identification of precise breeding targets to improve the seed quality of quinoa. The data presented in this study based on nutrient and transcriptome analyses contribute to an enhanced understanding of the genetic regulation of seed quality traits in quinoa and suggest high-precision candidate markers for such traits.

show abstract

“…Our results also demonstrate that X-ray based characterization methods can be effective, non-destructive tools to provide additional insights into seed morphology that are not possible using conventional microscopy. X-ray microtomography has been used in previous work to determine the proportions of different tissues of quinoa seeds and maize kernels [ 35 , 36 ] and also the microstructure of wheat-based food [ 37 , 38 ]. In the current work, 3D images of the external and internal structures of the grains were obtained by X-ray tomography allowing the volumes of the different grain tissues to be quantified.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of Grains and Flour Fractions from Field Grown Transgenic Oil-Accumulating Wheat Expressing Oat WRI1

Snell

Wilkinson

Taylor

et al. 2022

Plants

Self Cite

View full text Add to dashboard Cite

Wheat (Triticum aestivum L.) is one of the major staple crops in the world and is used to prepare a range of foods. The development of new varieties with wider variation in grain composition could broaden their use. We characterized grains and flours from oil-accumulating transgenic wheat expressing the oat (Avena sativa L.) endosperm WRINKLED1 (AsWRI1) grown under field conditions. Lipid and starch accumulation was determined in developing caryopses of AsWRI1-wheat and X-ray microtomography was used to study grain morphology. The developing caryopses of AsWRI1-wheat grains had increased triacylglycerol content and decreased starch content compared to the control. Mature AsWRI1-wheat grains also had reduced weight, were wrinkled and had a shrunken endosperm and X-ray tomography revealed that the proportion of endosperm was decreased while that of the aleurone was increased. Grains were milled to produce two white flours and one bran fraction. Mineral and lipid analyses showed that the flour fractions from the AsWRI1-wheat were contaminated with bran, due to the effects of the changed morphology on milling. This study gives a detailed analysis of grains from field grown transgenic wheat that expresses a gene that plays a central regulatory role in carbon allocation and significantly affects grain composition.

show abstract

Morpho-densitometric traits for quinoa (Chenopodium quinoa Willd.) seed phenotyping by two X-ray micro-CT scanning approaches

Cited by 27 publications

References 23 publications

Comparison of the Chemical and Technological Characteristics of Wholemeal Flours Obtained from Amaranth (Amaranthus sp.), Quinoa (Chenopodium quinoa) and Buckwheat (Fagopyrum sp.) Seeds

Comparison of the Chemical and Technological Characteristics of Wholemeal Flours Obtained from Amaranth (Amaranthus sp.), Quinoa (Chenopodium quinoa) and Buckwheat (Fagopyrum sp.) Seeds

Transcriptional Regulation of Quinoa Seed Quality: Identification of Novel Candidate Genetic Markers for Increased Protein Content

Characterisation of Grains and Flour Fractions from Field Grown Transgenic Oil-Accumulating Wheat Expressing Oat WRI1

Contact Info

Product

Resources

About