Morpho-cultural and pathogenic variability among isolates of Stemphylium vesicarium (Wallr.) E. Simmons, causing Stemphylium blight in onion collected from different geographical regions of Kashmir valley

Hassan, Mudasir; Yousuf, Vaseem; Bhat, Z. A.; Bhat, Nazir Ahmad; Shah, T. A.; Khan, M. A.; Mir, Reyazul Rouf; Rather, Roaf Ahmad; Shafi, Safoora

doi:10.1007/s42360-020-00253-8

Cited by 6 publications

(3 citation statements)

References 9 publications

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“…Small tan to brown water-soaked, oval lesions were observed, and leaf samples were collected for microscopic and molecular identification. The microscopic observation revealed the presence of ovoid to oblong, brown color conidia, and these morphological observations coincided with the previous reports ( Dangi et al., 2019 ; Gedefaw et al., 2019 ; Hassan et al., 2020 ). Also, the amplified product of the internal transcribed spacer (ITS) sequence, identified using NCBI BLAST, had 100% similarity with previously submitted sequences.…”

Section: Discussionsupporting

confidence: 92%

Screening of short-day onions for resistance to Stemphylium leaf blight in the seed-to-bulb stage (stage I) and bulb-to-seed stage (stage II)

Chandel

Kamil²,

Singh³

et al. 2022

Front. Plant Sci.

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Stemphylium leaf blight, caused by Stemphylium vesicarium, is a very important fungal disease in onions since its epidemics are able to affect both the bulb yield and the seed quality. The aim of this study was to screen onion genotypes at stage I (seed to bulb) and further screen the identified resistant and susceptible genotypes at stage II (bulb to seed). One hundred and fifty-seven genotypes were screened against SLB under artificially inoculated field conditions. Results revealed a significant variation among the morphological and biochemical traits studied. Correlation studies revealed a significant and negative correlation between percent disease incidence (PDI), pseudostem width, neck thickness, and dry matter. Fifteen genotypes were identified as moderately resistant, and the rest were categorized as susceptible. Bulbs of the genotypes, identified as moderately resistant, were again screened for resistance in stage II. All the genotypes were categorized as moderately susceptible. Biochemical analysis revealed that total foliar phenol content, pyruvic acid, catalase, and peroxidase increased up to 20 days after inoculation (DAI) and thereafter declined. Protein content was highest in the initial stage and declined at 10, 20, and 30 DAI. The higher biochemical activity was observed in moderately resistant category genotypes compared with the susceptible ones. Correlation analysis showed a highly significant and negative correlation of PDI with total foliar phenol content (TFPC), pyruvic acid, catalase, peroxidase, and protein content. To conclude, it was observed that screening against SLB should be done at both the stages (stage I and Stage II) to identify resistant onion genotypes. Direction selection for genotypes with high dry matter, higher phenols, and enzymes may be an alternative pathway to select genotypes for a robust resistance breeding program.

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Section: Discussionsupporting

confidence: 92%

Screening of short-day onions for resistance to Stemphylium leaf blight in the seed-to-bulb stage (stage I) and bulb-to-seed stage (stage II)

Chandel

Kamil²,

Singh³

et al. 2022

Front. Plant Sci.

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“…The number of scorable and polymorphic bands ranged from 7 to 160 and from 2 to 10, respectively (Table 2). Highest scored bands were 160 in SV-A04 with highest polymorphic band (10) showing 6.25 per cent polymorphism. The least scored bands were 7 in SV-F02 with least polymorphic bands (2) showing 28.57 per cent polymorphism.…”

Section: Polymorphism Using Random Amplified Polymorphic Dna (Rapd) Primersmentioning

confidence: 95%

“…In all thirty six cultures, proved pathogenic on susceptible host "Yellow Globe" and were named as Sv-01, Sv-02, Sv-03; Sv-04, Sv-05, Sv-06 ; Sv-07, Sv-08, Sv-09 from three different onion fields from wadura, Achabal and Pattan of district Baramulla respectively, Sv-10 Sv-11 Sv-12; Sv-13, Sv-14, Sv-15; Sv-16, Sv-17, Sv-18 from Shalimar, Danderkah, and Noorbagh of district Srinagar, respectively, Sv-19, Sv-20, Sv-21; Sv-22, Sv-23, Sv-24; Sv-25, Sv-26, Sv-27 isolates from Chadoora, Magam, and Ompora of district Budgam, respectively, Sv-28, Sv-29, Sv-30; Sv-31, Sv-32, Sv-33; Sv-34, Sv-35, Sv-36 from Sangam, Bijbhera and Larnoo of district Anantnag, respectively and were used for study of genetic diversity. The cultural, morphological and pathogenic variability of these isolates is discussed in Hassan et al [10] .…”

Section: Isolation and Pathogenicity Of S Vesicarium Isolatesmentioning

confidence: 99%

Genetic diversity of Stemphylium vesicarium causing Stemphylium blight of onion from different geographical regions of Kashmir

Hassan¹,

Yousuf²,

Rather³

et al. 2021

Pharma Innovation

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Stemphylium blight is the most serious and devastating disease of onion limiting the quality and quantity of both bulb and seed. Molecular analysis using 9 RAPD primers was done to assess the genetic diversity among the 36 S. vesicarium isolates from onion obtained from different geographical areas of Kashmir valley. All the 9 RAPD markers used during the present study revealed a moderate level of polymorphism in different isolates of S. vesicarium. In the present study maximum scored bands were 160 in SV-A04 marker with maximum polymorphic bands (10) showing 6.25 per cent polymorphism. The lowest scored bands were 7 in marker "SV-F02" with only two polymorphic bands showing 28.57 per cent polymorphism. In total, 579 bands were scored of which 54 were polymorphic, thereby showing 9.32 per cent polymorphism. Genetic dissimilarity of the isolates varied from 1.00 to 0.14.The present study provides insights into the extent of genetic diversity available in 36 S. vesicarium isolates from onion obtained from different geographical areas of Kashmir valley.

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First report of Stemphylium vesicarium causing leaf blight of onion in Slovakia

Kádasi Horáková,

Barta,

Tancik

et al. 2024

J Plant Dis Prot

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During the 2022 growing season, commercial crops of onion (Allium cepa cv. Medusa) were found to be affected by an extensive leaf blight in south western Slovakia. The pathogen present on diseased leaves was isolated and identified as Stemphylium vesicarium through morphological and molecular analyses, utilizing three DNA markers (ITS, tef1-α, and β-tubulin). A detailed description and illustration of the fungus are provided. Although this pathogen is already present in Slovakia, this is the first record of S. vesicarium causing leaf blight on A. cepa in the country. Pathogenicity tests with a fungal isolate were performed on three local cultivars of onion plants in the laboratory, indicating varying susceptibility of the cultivars to S. vesicarium.

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Morpho-cultural and pathogenic variability among isolates of Stemphylium vesicarium (Wallr.) E. Simmons, causing Stemphylium blight in onion collected from different geographical regions of Kashmir valley

Cited by 6 publications

References 9 publications

Screening of short-day onions for resistance to Stemphylium leaf blight in the seed-to-bulb stage (stage I) and bulb-to-seed stage (stage II)

Screening of short-day onions for resistance to Stemphylium leaf blight in the seed-to-bulb stage (stage I) and bulb-to-seed stage (stage II)

Genetic diversity of Stemphylium vesicarium causing Stemphylium blight of onion from different geographical regions of Kashmir

First report of Stemphylium vesicarium causing leaf blight of onion in Slovakia

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