Morphine-conditioned cue alters c-Fos protein expression in the brain of crayfish

Dziopa, Leah; Imeh-Nathaniel, Adebobola; D, Baier; Kiel, Michael; Sayeed, Sameera; Brager, Adam; Beatriz, Vega; Nathaniel, Thomas I.

doi:10.1016/j.brainresbull.2011.04.003

Cited by 13 publications

(7 citation statements)

References 91 publications

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“…CPP was not established in the initially preferred soft textured environment when compared with the control group (Imeh-Nathaniel et al, 2016 ). Similarly, in a study investigating the effects of different visual cues on CPP when paired with morphine, crayfish initially showed an unconditioned preference for a white walled environment (Dziopa et al, 2011 ). After conditioning, crayfish showed preference for striped environment when paired with single and multiple morphine injections, at all doses.…”

Section: Novel Stimuli Directly Augment Exploration and Appetitive Momentioning

confidence: 99%

“…The c-Fos protein has been studied in mammals in regard to activation of brain regions by drugs of abuse and, when activated, plays a role in signal transduction and genetic modifications. This protein has not been studied extensively in invertebrate models, but an investigation of c-fos gave insights into the molecular alterations associated with drug reward in invertebrates (Dziopa et al, 2011 ). The single and repeated injections of morphine at 3.0, 6.0, and 12.0 μg/g (Figure 3 ) in an unconditioned experiment did not reveal a significant increase in c-Fos expression.…”

Section: Molecular Alterations Associated With Drug Conditioned Rewarmentioning

confidence: 99%

“…The levels of c-Fos expression remained constant in the control group. This result suggests that novel environment when paired with drugs impacts gene regulatory processes (Dziopa et al, 2011 ).…”

Section: Molecular Alterations Associated With Drug Conditioned Rewarmentioning

confidence: 99%

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The Sensitivity of the Crayfish Reward System to Mammalian Drugs of Abuse

et al. 2017

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The idea that addiction occurs when the brain is not able to differentiate whether specific reward circuits were triggered by adaptive natural rewards or falsely activated by addictive drugs exist in several models of drug addiction. The suitability of crayfish (Orconectes rusticus) for drug addiction research arises from developmental variation of growth, life span, reproduction, behavior and some quantitative traits, especially among isogenic mates reared in the same environment. This broad spectrum of traits makes it easier to analyze the effect of mammalian drugs of abuse in shaping behavioral phenotype. Moreover, the broad behavioral repertoire allows the investigation of self-reinforcing circuitries involving appetitive and exploratory motor behavior, while the step-wise alteration of the phenotype by metamorphosis allows accurate longitudinal analysis of different behavioral states. This paper reviews a series of recent experimental findings that evidence the suitability of crayfish as an invertebrate model system for the study of drug addiction. Results from these studies reveal that unconditioned exposure to mammalian drugs of abuse produces a variety of stereotyped behaviors. Moreover, if presented in the context of novelty, drugs directly stimulate exploration and appetitive motor patterns along with molecular processes for drug conditioned reward. Findings from these studies indicate the existence of drug sensitive circuitry in crayfish that facilitates exploratory behavior and appetitive motor patterns via increased incentive salience of environmental stimuli or by increasing exploratory motor patterns. This work demonstrates the potential of crayfish as a model system for research into the neural mechanisms of addiction, by contributing an evolutionary, comparative context to our understanding of natural reward as an important life-sustaining process.

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Section: Novel Stimuli Directly Augment Exploration and Appetitive Momentioning

confidence: 99%

Section: Molecular Alterations Associated With Drug Conditioned Rewarmentioning

confidence: 99%

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The Sensitivity of the Crayfish Reward System to Mammalian Drugs of Abuse

et al. 2017

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“…Indeed, crayfish exposure to cocaine, morphine and methamphetamine increases mobility (Imeh‐Nathaniel et al, ), whereas exposure to d ‐amphetamine stimulates exploration (Alcaro et al, ). Finally, exposure of crayfish to morphine increases locomotor activity acutely but reduces it at higher and/or chronic doses (Dziopa et al, ).…”

Section: Measuring Affective‐like Behavior In Crayfish and Zebrafishmentioning

confidence: 99%

Emotional behavior in aquatic organisms? Lessons from crayfish and zebrafish

Abreu

Maximino

Banha

et al. 2019

J of Neuroscience Research

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Experimental animal models are a valuable tool to study the neurobiology of emotional behavior and mechanisms underlying human affective disorders. Mounting evidence suggests that various aquatic organisms, including both vertebrate (e.g., zebrafish) and invertebrate (e.g., crayfish) species, may be relevant to study animal emotional response and its deficits. Ideally, model organisms of disease should possess considerable genetic and physiological homology to mammals, display robust behavioral and physiological responses to stress, and should be sensitive to a wide range of drugs known to modulate stress and affective behaviors. Here, we summarize recent findings in the field of zebrafish‐ and crayfish‐based tests of stress, anxiety, aggressiveness and social preference, and discuss further perspectives of using these novel model organisms in translational biological psychiatry. Outlining the remaining questions in this field, we also emphasize the need in further development and a wider use of crayfish and zebrafish models to study the pathogenesis of affective disorders.

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“…Molecular, neurophysiological, and neurobehavioral experimentation ( Clarac and Pearlstein, 2007 ) on the mechanisms of natural and drug-sensitive reward profits from a highly modular neural structure, conserved monoaminergic, neuromodulatory systems, a relatively small number of large and individually identifiable neurons, and high sensitivity toward human drugs of abuse. Amphetamine ( Alcaro et al, 2011 ), cocaine ( Nathaniel et al, 2012a , b ), morphine ( Nathaniel et al, 2010 ), and cathinones (Gore et al, unpublished data) exhibit potent psychostimulant properties, which sensitize with repeated exposure ( Nathaniel et al, 2010 , 2012b ; Dziopa et al, 2011 ). Moreover, in a conditioned place preference paradigm (CPP), these substances trigger the formation of strong associations between drugs and the cues with which they are paired ( Panksepp and Huber, 2004 ; Nathaniel et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Crayfish Self-Administer Amphetamine in a Spatially Contingent Task

2018

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Natural reward is an essential element of any organism’s ability to adapt to environmental variation. Its underlying circuits and mechanisms guide the learning process as they help associate an event, or cue, with the perception of an outcome’s value. More generally, natural reward serves as the fundamental generator of all motivated behavior. Addictive plant alkaloids are able to activate this circuitry in taxa ranging from planaria to humans. With modularly organized nervous systems and confirmed vulnerabilities to human drugs of abuse, crayfish have recently emerged as a compelling model for the study of the addiction cycle, including psychostimulant effects, sensitization, withdrawal, reinstatement, and drug reward in conditioned place preference paradigms. Here we extend this work with the demonstration of a spatially contingent, operant drug self-administration paradigm for amphetamine. When the animal enters a quadrant of the arena with a particular textured substrate, a computer-based control system delivers amphetamine through an indwelling fine-bore cannula. Resulting reward strength, dose-response, and the time course of operant conditioning were assessed. Individuals experiencing the drug contingent on their behavior, displayed enhanced rates of operant responses compared to that of their yoked (non-contingent) counterparts. Application of amphetamine near the supra-esophageal ganglion elicited stronger and more robust increases in operant responding than did systemic infusions. This work demonstrates automated implementation of a spatially contingent self-administration paradigm in crayfish, which provides a powerful tool to explore comparative perspectives in drug-sensitive reward, the mechanisms of learning underlying the addictive cycle, and phylogenetically conserved vulnerabilities to psychostimulant compounds.

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Morphine-conditioned cue alters c-Fos protein expression in the brain of crayfish

Cited by 13 publications

References 91 publications

The Sensitivity of the Crayfish Reward System to Mammalian Drugs of Abuse

The Sensitivity of the Crayfish Reward System to Mammalian Drugs of Abuse

Emotional behavior in aquatic organisms? Lessons from crayfish and zebrafish

Crayfish Self-Administer Amphetamine in a Spatially Contingent Task

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