Moringa oleifera Leaf Extract Promotes Healing of Infected Wounds in Diabetic Rats: Evidence of Antimicrobial, Antioxidant and Proliferative Properties

Abstract

Moringa oleifera is known to possess wound healing activity. The present study evaluated the healing properties of methanolic extract of M. oleifera leaves in excision wounds infected with methicillin-resistant Staphylococcus aureus (MRSA) or P. aeruginosa in diabetic rats. An in vitro study was also carried out to determine the gene expression of VEGF and TGF-β1. Preliminary phytochemical and GC-MS analyses were carried out to determine different chemical constituents present in the extract. M. oleifera was applied locally as an ointment at two different concentrations. Wound contraction, period of epithelization, antioxidant enzyme activities and histological changes were determined. For the gene expression study, HaCaT cell lines were used. The formulation of M. oleifera extract improved wound contraction and decreased the period of epithelization, which was associated with an increase in antioxidant enzyme activities, epithelization, capillary density and collagen formation in MRSA-infected diabetic rats. However, this effect was reduced in diabetic animals infected with P. aeruginosa. An increase in the expression of VEGF and TGF-β1 was observed in HaCaT cell lines. M. oleifera extract promotes the healing of infected wounds in MRSA-infected diabetic rats but is less effective in the healing of wounds infected with P. aeruginosa in diabetic rats.

Effect of Moringa oleifera Leaf Extract on Excision Wound Infections in Rats: Antioxidant, Antimicrobial, and Gene Expression Analysis

“…The results of the current study on infected wounds in normal rats support our findings on the effect of M. oleifera extract on the infected wounds in diabetic rats [ 19 ]. In our earlier study, we analyzed the chemical constituents of M. oleifera by GC-MS to show the presence of volatile constituents.…”

“…The cytotoxicity of the extract and its effect on the expression of VEGF and TGF-β1 genes were evaluated using HaCaT cells. The study was a continuation of our report on the effect of M. oleifera extract on infected wounds in diabetic rats [ 19 ].…”

“…Therefore, different phytochemicals and novel bioactive compounds are under investigation to treat wound infections to control multi-drug resistant microbial pathogens [ 18 ]. We have previously reported the effect of M. oleifera extract on diabetic wound infection in rats wherein a significant wound healing effect was observed [ 19 ]. In this study, the wound healing potential of M. oleifera extract on excision wound model inoculated with either MRSA or P. aeruginosa in normal rats was determined along with its cytotoxicity and effect on expression of the vascular endothelial growth factor (VEGF) and transforming growth factor-beta 1 (TGF-β1) genes on human keratinocyte (HaCaT) cell lines under in vitro conditions.…”

Green Biosynthesis of Silver Nanoparticles from Moringa oleifera Leaves and Its Antimicrobial and Cytotoxicity Activities

“…Also, [ 19 ] mentioned the ecologically friendly manufacture of silver nanoparticles from fresh leaves of the Moringa oleifera plant and evaluated against Escherichia coli , Klebsiella pneumonia , Staphylococcus aureus, and Bacillus subtilis . Moringa contains a long list of medical use including antibacterial [ 20 ], antifungal [ 21 ], antiparasitic [ 22 ], antiviral [ 23 ], wound healing, antimicrobial, antioxidant, and proliferative properties [ 24 ]. In the present study, we evaluated the effectiveness of the synthesis of silver nanoparticles from aqueous extract of Moringa oleifera leaves extract against the clinical strain of bacteria (positive and negative Gram) and human pathogenic fungi ( Candida species)and evaluated the cytotoxicity effect of silver nanoparticles on human skin cancer.…”

Green Production and Interaction of Carboxylated CNTs/Biogenic ZnO Composite for Antibacterial Activity

“…These phytoconstituents act as bioactive organic compounds such as alkaloids, flavonoids, glycosides, phenols, saponins, tannins, terpenoids, reducing sugars, and triglycerides [ 4 ]. Most of the previous studies on Moringa oleifera have shown that the presence of phenolic compounds contributes to different antimicrobial and antioxidant properties [ 5 , 6 , 7 , 8 , 9 ]. Furthermore, the extraction of these phytochemicals through different solvents shows a very effective inhibition activity against microbes [ 10 ].…”