Moringa oleifera extract attenuates the CoCl2 induced hypoxia of rat's brain: Expression pattern of HIF-1α, NF-kB, MAO and EPO

Mohamed, Amany Abdel-Rahman; Metwally, Mohamed M.M.; Khalil, Samah R.; Salem, Gamal A.; Ali, Haytham

doi:10.1016/j.biopha.2018.11.019

Cited by 52 publications

(23 citation statements)

References 56 publications

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“…This modulation was associated with down-regulation of the mRNA expression profile of genes that mediate the inflammatory response in the kidney tissue. Previously, various antioxidants of natural sources have shown an ameliorative efficacy against xenobiotics-induced toxicity in in vivo animal studies [11,[37][38][39][40].…”

Section: Discussionmentioning

confidence: 99%

“…The 1st group (Control) received only distilled water via oral administration. The 2nd group (MOEE) orally received 400 mg MOEE/kg bw/day for six weeks [11]. The 3rd group (CoCl 2 ) received 300 mg/L freshly prepared CoCl 2 solution in drinking water for four weeks [29].…”

Section: Experimental Animals and Proceduresmentioning

confidence: 99%

“…The selected dose of CoCl2 in this study was carried out based on the results of previous work of Awoyemi et al [29], where it induced tissue damage via oxidative stress, inflammation, and apoptosis in a dose-dependent manner. The ethanolic extract of MO leaves was found to increase the protective activity of the antioxidant system and slow the pathological development of tissue alterations, which was induced by xenobiotic exposure when administrated at the level of 400 mg/kg bw [11,25]. At the end of the experimental period, the final body weight of rats in all groups was measured to estimate the changes in body weight.…”

Section: Experimental Animals and Proceduresmentioning

confidence: 99%

“…The systemic health effects are likely to occur at blood Co concentrations higher than 300 µg/L [8]. Mostly, cobalt toxicity investigations have been mainly performed for its genotoxicity [9], tissue hypoxia [10,11], and cytotoxicity [12].…”

Section: Introductionmentioning

confidence: 99%

“…A wide degree of safety of different MO extracts was reported, such as for example the median lethal dose (LD 50 ) of alcoholic extract of MO leaves up to 5 gm/ kg body weight (bw) [21]. in vivo human and animals as well as in vitro studies proved that various extracts of MO leaves possess different therapeutic effects, such as anti-inflammatory, hypolipidemic, antioxidant, anti-diabetic, and chemoprotective (hepatic, cardiac, renal, and nervous) activities [11,[22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

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Ethanolic Extract of Moringa oleifera Leaves Influences NF-κB Signaling Pathway to Restore Kidney Tissue from Cobalt-Mediated Oxidative Injury and Inflammation in Rats

et al. 2020

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This study aimed to describe the protective efficacy of Moringa oleifera ethanolic extract (MOEE) against the impact of cobalt chloride (CoCl2) exposure on the rat’s kidney. Fifty male rats were assigned to five equal groups: a control group, a MOEE-administered group (400 mg/kg body weight (bw), daily via gastric tube), a CoCl2-intoxicated group (300 mg/L, daily in drinking water), a protective group, and a therapeutic co-administered group that received MOEE prior to or following and concurrently with CoCl2, respectively. The antioxidant status indices (superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH)), oxidative stress markers (hydrogen peroxide (H2O2), 8-hydroxy-2-deoxyguanosine (8-OHdG), and malondialdehyde (MDA)), and inflammatory response markers (nitric oxide (NO), tumor necrosis factor (TNF-α), myeloperoxidase (MPO), and C-reactive protein (CRP)) were evaluated. The expression profiles of pro-inflammatory cytokines (nuclear factor-kappa B (NF-kB) and interleukin-6 (IL-6)) were also measured by real-time quantitative polymerase chain reaction (qRT-PCR). The results showed that CoCl2 exposure was associated with significant elevations of oxidative stress and inflammatory indices with reductions in the endogenous tissue antioxidants’ concentrations. Moreover, CoCl2 enhanced the activity of the NF-κB inflammatory-signaling pathway that plays a role in the associated inflammation of the kidney. MOEE ameliorated CoCl2-induced renal oxidative damage and inflammatory injury with the suppression of the mRNA expression pattern of pro-inflammatory cytokine-encoding genes. MOEE is more effective when it is administered with CoCl2 exposure as a prophylactic regimen. In conclusion, MOEE administration exhibited protective effects in counteracting CoCl2-induced renal injury in rats.

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Section: Discussionmentioning

confidence: 99%

Section: Experimental Animals and Proceduresmentioning

confidence: 99%

Section: Experimental Animals and Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ethanolic Extract of Moringa oleifera Leaves Influences NF-κB Signaling Pathway to Restore Kidney Tissue from Cobalt-Mediated Oxidative Injury and Inflammation in Rats

et al. 2020

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Medicinal utilization and nutritional properties of drumstick (Moringa oleifera)—A comprehensive review

Zarina,

Wani,

Rawat

et al. 2024

Food Science & Nutrition

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The tropical and subtropical regions of the world support the growth of the Indian plant Moringa oleifera. It usually goes by the name drumstick tree or horseradish tree and thrives in warm climates. The leaves of the M. oleifera tree are now frequently used as nutrients and nutraceuticals due to their availability of various minerals. While having only very minor antinutritional effects, the leaves are abundant in many beneficial compounds. A recent review of the bioactive components and activity of moringa leaves has focused on both in vivo and in vitro studies. Drumstick leaves have antidiabetic qualities, anti‐inflammatory, anticancer, and antibacterial qualities among other health benefits. Phytochemicals, in addition to minerals and vitamins, are abundant in this vegetable. The majority of these effects, according to a review in the literature, are mostly brought on by the presence of carotenoids, glucosinolates, and phytochemicals. As a value‐added component in the production of wholesome meals, moringa is becoming more popular. Despite extensive research into locating and quantifying these advantageous elements in drumstick leaves, bioavailability and bioaccessibility studies were carried out. Beneficial photochemicals are absorbed and digested through incredibly intricate processes that involve several physicochemical and physiological interactions. Therefore, the biological impact of food may be attributed to its various metabolites that can access particular areas of action rather than its original substances. This body of literature offers the most recent findings in scientific research on the bioavailability, health advantages, nutritional profiles, and bioactive activities of moringa leaves as they relate to their use in a range of food products. Drumsticks are frequently used as a food element that promotes health because of their potent protection against a variety of ailments and the presence of environmental pollutants.

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Vascular characteristics and expression of hypoxia genes in Tibetan pigs’ hearts

Yang

Gao

Yang

et al. 2021

Veterinary Medicine & Sci

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Background: Tibetan pigs have exhibited unique characteristics from low-altitudes pigs and adapted well to the Qinghai-Tibet Plateau. Objectives:The current study was undertaken to investigate the hypoxic adaptation of heart in Tibetan pigs. Methods:The hearts of Tibetan pigs and Landrace pigs raised at high or low altitudes were compared using 3D casting technology, scanning electron microscopy and realtime quantitative PCR (qRT-PCR). Results:We found that the ratio of the major axis to the minor axis and the density of the heart were significantly higher in Tibetan pigs than in Landrace pigs (p < 0.05).Tibetan pigs had larger diameters and higher densities of arterioles than Landrace pigs (p < 0.05), and these features have a similar variation with the expression of vascular endothelial growth factor (VEGF). The cardiac expression levels of hypoxia-inducible factor-1α (HIF-1α) and endothelial nitric oxide synthase (eNOS) were significantly higher in pigs reared at high altitudes than in those reared at low altitudes (p < 0.05).In contrast, Egl nine homolog 1 (EGLN1) had the opposite trend with respect to HIF-1α and eNOS and was related to red blood cell (RBC) counts. Notably, the expressions of erythropoietin (EPO) and endothelial PAS domain-containing protein 1 (EPAS1) were significantly higher in Landrace pigs kept at high altitudes than in the others (p < 0.05) and were associated with haemoglobin.Conclusions: These findings show that the regulation of the heart function of Tibetan pigs in a hypoxic environment is manifested at various levels to ensure the circulation of blood under extreme environmental conditions.

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Moringa oleifera extract attenuates the CoCl2 induced hypoxia of rat's brain: Expression pattern of HIF-1α, NF-kB, MAO and EPO

Cited by 52 publications

References 56 publications

Ethanolic Extract of Moringa oleifera Leaves Influences NF-κB Signaling Pathway to Restore Kidney Tissue from Cobalt-Mediated Oxidative Injury and Inflammation in Rats

Ethanolic Extract of Moringa oleifera Leaves Influences NF-κB Signaling Pathway to Restore Kidney Tissue from Cobalt-Mediated Oxidative Injury and Inflammation in Rats

Medicinal utilization and nutritional properties of drumstick (Moringa oleifera)—A comprehensive review

Vascular characteristics and expression of hypoxia genes in Tibetan pigs’ hearts

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