Morin Reation for Beryllium

Sandell, E. B.

doi:10.1021/ac50152a027

Cited by 16 publications

(10 citation statements)

References 26 publications

(5 reference statements)

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“…However, in the morin method of analysis conditions may be chosen so that aluminum does not interfere, and quantities of the order of 0.001 p.p.m. can be detected (7). Traces of acetylacetone which are carried into the hydrochloric acid interfere with the fluorescence (5).…”

Section: Methodsmentioning

confidence: 99%

Separation of Beryllium from Biological Material

Toribara¹,

Chen²

1952

Anal. Chem.

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The methods used for the quantitative determination of small quantities of beryllium are subject to interferences from other ions. The existing schemes for separating the beryllium are tedious and not entirely quantitative. Some losses of beryllium during ashing had been reported, and a thorough investigation of this important step was desirable.The radioisotope Be7 was used for the studies, and by utilizing carrier-free isotope quantities (less than 10-10 gram) undetectable by any chemical or spectrographic method were investigated. Quantities of this magnitude could be separated completely from urine, soft tissues, and even bone if the extraction time were suitably increased. The separation is IN THE analysis of biological specimens for toxic materials, the LDM (a statistical term denoting the dose which, on the average, causes 50% mortality) is important in determining the sensitivity required of analytical procedures. Beryllium, administered intravenously, is very toxic; the LDw is approximately 0.36 mg. per kg. (8). Thus, for distribution and excretion experiments, not more than 0.2 mg. per kg. can be administered. Ber-

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Section: Methodsmentioning

confidence: 99%

Separation of Beryllium from Biological Material

Toribara¹,

Chen²

1952

Anal. Chem.

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“…The most sensitive chemical test for beryllium is the fluorescence developed by its interaction with morin at pH's greater than 11, and considerable interest (10,12,15,24) has been shown in this reagent for estimating small quantities of the element.…”

Section: Methods Of Estimationmentioning

confidence: 99%

Analytical Chemistry of Micro Quantities of Beryllium

Toribara¹,

Sherman²

1953

Anal. Chem.

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Because of the very toxic nature of beryllium, submicrogram quantities of the element are of biological importance. Colorimetric, fluorimetric, and spectrographic methods used to determine small quantities of beryllium are subject to interferences by other elements, often necessitating a separation scheme to isolate the element before measurement. The fluorimetric method based on the interaction of beryllium with purified morin is the most sensitive chemical determination tested and compares favorably in sensitivity with the spectrographic method. The separation of beryllium from bone proved to be most difficult because of the large quantity of calcium phosphate. Using a combination of steps consisting of precipitation, electrolysis with a mercury cathode, absorption on an ion exchange resin, and complexing with acetylacetone, it was possible to isolate completely the smallest measurable quantity of beryllium from all biological samples. The radioisotope beryllium-7 was employed in the determination of the efficiency of each step of the separation scheme.

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“…F., Analyst, 70, 48 (1945). (7) Kamlet, J. (to Miles Laboratories, Inc.), U. S. Patent 2,406,774 (June 28, 1943).…”

Section: Literature Citedmentioning

confidence: 99%

“…Industrial exposure to beryllium is frequently associated with exposure to zinc or copper. Sandell (7) has shown that zinc causes fluorescence with morin, while copper destroys the fluorescence by oxidation of the dye. The authors have tested for possible interference by adding 10 mg. of these two metals as sulfates to urine.…”

Section: Recoverymentioning

confidence: 99%