MoRic8 Is a Novel Component of G-Protein Signaling During Plant Infection by the Rice Blast Fungus<i>Magnaporthe oryzae</i>

Li, Ya; Xia, Yan; Wang, Hong; Shen, Liang; Ma, Weibin; MinYan, Fang; Talbot, Nicholas J.; Wang, Zheng-Yi

doi:10.1094/mpmi-23-3-0317

Cited by 41 publications

(44 citation statements)

References 45 publications

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“…In addition to the membrane-localized GPCRs, cytoplasmic nonreceptor GEFs, such as N. crassa RIC-8 (635), have recently been identified which bind to the G-protein and accelerate guaninenucleotide exchange. In M. oryzae, MoRic8 was shown to interact with and positively regulate MagB (636), and in N. crassa RIC-8 exhibited GEF activity toward the GNA-1 and GNA-3 G␣ proteins (635). Another protein with GEF activity, Arr4p/Get3p, has been described in S. cerevisiae.…”

Section: The Heterotrimeric G-protein Pathwaymentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

159

186

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SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

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Section: The Heterotrimeric G-protein Pathwaymentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

159

186

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“…Deletion of the ricA gene (a RIC8 homolog) leads to impaired colony growth and total or partial loss of asexual sporulation in Aspergillus nidulans and Aspergillus fumigatus, respectively (27). In the rice blast fungus Magnaporthe oryzae, MoRic8, a Ric8 homolog, interacts with the G␣ protein MoMagB to regulate appressorial differentiation (28). Interestingly, no Ric8 homologs have been found in any plant species or in the budding yeast Saccharomyces cerevisiae.…”

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confidence: 99%

A Ric8/Synembryn Homolog Promotes Gpa1 and Gpa2 Activation To Respectively Regulate Cyclic AMP and Pheromone Signaling in Cryptococcus neoformans

2014

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The G protein ␣ subunits Gpa1, Gpa2, and Gpa3 mediate signal transduction and are important in the growth and virulence of Cryptococcus neoformans. To understand how Gpa1 functions without a conventional G␤ subunit, we characterized a resistance to inhibitors of cholinesterase 8 (Ric8) homolog from C. neoformans, which shares amino acid sequence homology with other Ric8 proteins that exhibit guanine nucleotide exchange factor (GEF) activity toward G␣. We found that the ric8 mutant was reduced in capsule size and melanin formation, which could be suppressed by cyclic AMP (cAMP) supplementation or by introducing the activated GPA1 Q284L allele. Consistent with the fact that Ric8 participates in cAMP signaling to regulate virulence, the ric8 mutant was attenuated in virulence toward mice. Interestingly, disruption of RIC8 also resulted in opposing effects on pheromone signaling, as the ric8 mutant showed reduced mating but an enhanced ability to induce the pheromone response in the mating partner. To identify Ric8 functional mechanisms, we examined the interactions between Ric8 and the three G␣ proteins. Ric8 interacted with Gpa1 and Gpa2, but not Gpa3. The presence of Gpa1 Q284L negatively affected its interaction with Ric8, whereas the activated Gpa2 Q203L allele abolished the interaction. Collectively, these findings suggest that Ric8 functions as a GEF to facilitate the activation of Gpa1-cAMP signaling and to promote Gpa2, affecting mating efficiency. Our study highlights the distinct and conserved characteristics associated with G protein signaling and contributes to our overall understanding of how G protein ␣ subunits function with or without a canonical G␤ partner in C. neoformans.

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“…As shown in Fig. 5C, 35 S-labeled AnGanB, but not AnFadA or AnGanA, could be copurified with GST-AnRicA specifically, indicating that AnRicA directly binds to AnGanB in vitro. Collectively, these data suggest that GanB is a primary target of RicA-mediated signaling in A. nidulans, and the full-length AnRicA and AnGanB are necessary for their physical interaction.…”

Section: Resultsmentioning

confidence: 75%

“…The AnricA ORF was fused with GST in the pGEX 5X-1 vector (GE Healthcare), and the AnRicA protein was expressed and purified in Escherichia coli. The ORF regions of AnfadA, AnganA, and AnganB were cloned under the T7 promoter of pCDNA3, and each G␣ subunit was translated in vitro and labeled with 35 S. An equal amount of in vitro-translated proteins was added to glutathione bead-GST-AnRicA or glutathione bead-GST (control) suspensions and subjected to pulldown. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

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The Putative Guanine Nucleotide Exchange Factor RicA Mediates Upstream Signaling for Growth and Development in Aspergillus

et al. 2012

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dHeterotrimeric G proteins (G proteins) govern growth, development, and secondary metabolism in various fungi. Here, we characterized ricA, which encodes a putative GDP/GTP exchange factor for G proteins in the model fungus Aspergillus nidulans and the opportunistic human pathogen Aspergillus fumigatus. In both species, ricA mRNA accumulates during vegetative growth and early developmental phases, but it is not present in spores. The deletion of ricA results in severely impaired colony growth and the total (for A. nidulans) or near (for A. fumigatus) absence of asexual sporulation (conidiation). The overexpression (OE) of the A. fumigatus ricA gene (AfricA) restores growth and conidiation in the ⌬AnricA mutant to some extent, indicating partial conservation of RicA function in Aspergillus. A series of double mutant analyses revealed that the removal of RgsA (an RGS protein of the GanB G␣ subunit), but not sfgA, flbA, rgsB, or rgsC, restored vegetative growth and conidiation in ⌬AnricA. Furthermore, we found that RicA can physically interact with GanB in yeast and in vitro. Moreover, the presence of two copies or OE of pkaA suppresses the profound defects caused by ⌬AnricA, indicating that RicA-mediated growth and developmental signaling is primarily through GanB and PkaA in A. nidulans. Despite the lack of conidiation, brlA and vosA mRNAs accumulated to normal levels in the ⌬ricA mutant. In addition, mutants overexpressing fluG or brlA (OEfluG or OEbrlA) failed to restore development in the ⌬AnricA mutant. These findings suggest that the commencement of asexual development requires unknown RicA-mediated signaling input in A. nidulans.

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MoRic8 Is a Novel Component of G-Protein Signaling During Plant Infection by the Rice Blast FungusMagnaporthe oryzae

Cited by 41 publications

References 45 publications

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

A Ric8/Synembryn Homolog Promotes Gpa1 and Gpa2 Activation To Respectively Regulate Cyclic AMP and Pheromone Signaling in Cryptococcus neoformans

The Putative Guanine Nucleotide Exchange Factor RicA Mediates Upstream Signaling for Growth and Development in Aspergillus

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