More than efficacy revealed by single-cell analysis of antiviral therapeutics

Liu, Wu; Caglar, Mehmet Umut; Mao, Zhangming; Woodman, Andrew; Arnold, Jamie J.; Wilke, Claus O.; Cameron, Craig Ε.

doi:10.1126/sciadv.aax4761

Cited by 19 publications

(14 citation statements)

References 42 publications

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“…To record and quantify live virus egress from a single cell, a chip that combines cell capture and virus detection was needed. There is an extensive literature on single-cell trapping, mainly for intracellular analyses, demonstrating that a PDMS device is a suitable system for cell culture and that immobilization does not alter cell properties ( 1 , 2 , 14 , 20 , 45 , 46 , 47 , 48 ). To analyze the VLP produced from each individual cell, we took advantage of the flow to separate and move the newly formed fluorescent VLPs away from the fluorescent-producing cells.…”

Section: Resultsmentioning

confidence: 99%

Viro-fluidics: Real-time analysis of virus production kinetics at the single-cell level

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Socol

Naillon

et al. 2022

Biophysical Reports

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Section: Resultsmentioning

confidence: 99%

Viro-fluidics: Real-time analysis of virus production kinetics at the single-cell level

Eid

Socol

Naillon

et al. 2022

Biophysical Reports

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“…In this way we can identify how a virus modifies the transcriptome of the host cells, and how the host responds to the infection at single-cell level. Finally, microfluidic and onchip investigation strategies are also promising 143 , and one of the best examples is the characterization of molecular inhibitors of human enteroviruses after screening thousands of individual virus-cell interactions 144 . Studying free single viral particles is very informative, however, looking at individual infected cells with different techniques provides complementary insights into the biology of viruses that are difficult to obtain when viruses are `decontextualized´ from their hosts.…”

Section: Pairing Viruses With Their Hostsmentioning

confidence: 99%

Single-virus genomics and beyond

2020

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Viruses are extremely diverse and modulate important biological and ecological processes globally. However, much of viral diversity remains uncultured and yet to be discovered.Several powerful culture-independent tools, in particular metagenomics, have substantially advanced virus discovery. Among those tools is single-virus genomics, which yields sequenced reference genomes from individual sorted virus particles without the need for cultivation. This new method complements virus culturing and metagenomic approaches and its advantages include, for example, targeted investigation of specific virus groups and investigation of genomic microdiversity within viral populations. In this Review, we provide a brief history of single-virus genomics, outline how this emergent method has facilitated advances in virus ecology, and discuss its current limitations and future potential. Finally, we address how this method may synergistically intersect with other single-virus and single-cell approaches.

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“…Num interessante diálogo com Eugene Garfield, em 1974, o sociólogo Robert Merton (citado em Csiszar, 2020 antecipou a que ficaria conhecida como lei de Goodhart 13 e fez uma previsão que se viria a revelar acertada:…”

Section: Dois Sintomas Da Mesma Doença: O Abuso Das Métricas Na Publicação E Avaliaçãounclassified

“…Similarly, a microfluidic chip containing multiple micro-cavities was recently reported to assess the viral infection dynamics and inhibition on individual cells infected with enterovirus [13]. The chip was employed to investigate three classes of enterovirus inhibitors with distinct mechanisms of action.…”

Section: Drug Screening and Discoverymentioning

confidence: 99%

See 1 more Smart Citation

A Universidade do Minho em tempos de pandemia: Tomo II: Re(Ações)

Martins

Rodrigues

2020

A Universidade Do Minho Em Tempos De Pandemia

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Nesta obra reúnem-se cerca de meia centena de textos que retratam, a partir de diferente experiências, perspetivas e olhares disciplinares, a forma como a pandemia provocada pelo vírus SARS-CoV-2 foi sentida na Universidade do Minho, a primeira universidade portuguesa a ser atingida pelos seus efeitos, a partir de 7 de março de 2020. Os contributos aqui reunidos exprimem o modo como a comunidade académica se organizou para garantir o funcionamento da instituição e o cumprimento da sua missão, quer no âmbito do ensino, quer da investigação e da inovação, não dispensando uma necessária reflexão crítica sobre a nova realidade que se afirmou com a pandemia, o seu significado e os impactos da mesma na sociedade. Dado o elevado número e a diversidade dos contributos, a obra organiza-se em três volumes, que tentam dar resposta a três possíveis interrogações. Assim, o 1º volume, com o subtítulo de ‘Reflexões’, procura equacionar diferentes perspetivas em torno da questão: Mas o que é isto? Já o 2º volume, que recebe o subtítulo de ‘(Re)Ações’, corporiza um conjunto de textos que reflete as atuações em diferentes áreas da dimensão académica, elucidando quanto ao modo: Como reagimos? Finalmente, o 3º volume, que acusa o subtítulo de ‘Projeções’, dá expressão a uma inevitável pergunta: E agora? Pois, afinal, todos sabemos que isto não vai, nem pode, ficar tudo bem.

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More than efficacy revealed by single-cell analysis of antiviral therapeutics

Abstract: Single-cell analysis of antivirals reveals efficacy, mechanism of action, and, perhaps, the likelihood of resistance.

Cited by 19 publications

References 42 publications

Viro-fluidics: Real-time analysis of virus production kinetics at the single-cell level

Viro-fluidics: Real-time analysis of virus production kinetics at the single-cell level

Single-virus genomics and beyond

A Universidade do Minho em tempos de pandemia: Tomo II: Re(Ações)

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