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Brindl, Helen M.; Dempsey, William J.; Roberts, Holland D.; Rand, Helen; Knight, Pearle E.; Traxler, Arthur E.

doi:10.2307/805969

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1998

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“…Furthers studies of these antibodies showed that they reacted with the carbohydrate epitopes of LPS, the major immunogenic component of L. pneuomophila [3].…”

Section: Discussionmentioning

confidence: 99%

Monoclonal antibodies directed against lipopolysaccharide of legionella pneumophila serogroup 1

et al. 1998

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SUMMARYThe monoclonal antibodies (MAbs) against lipopolysaccharide of virulent strain of Legionella pneumophila serogroup 1 were produced. Three most productive hybrid clones (5F4~ 5F10 and 2C9) were selected from fusions of mouse myeloma cells with spleen cells from BALB/c mice, immunized with bacterial outer membrane antigens. All generated clones were IgGsecreting. The MAbs had narrow strain specificity and showed no cross-reactions with other unrelated bacterial species. These antibodies were tested in sandwich ELISA. The results suggest that the MAbs could be used for diagnostic purposes.

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“…Furthers studies of these antibodies showed that they reacted with the carbohydrate epitopes of LPS, the major immunogenic component of L. pneuomophila [3].…”

Section: Discussionmentioning

confidence: 99%