More Practical Problem Solving in HPLC

Abstract

This book was carefully produced. Nevertheless, authors and publisher do not warrant the information contained therein to be free of errors. Readers are advised to keep in mind that statements, data, illustrations, procedural details or other items may inadvertently be inaccurate. Library of Congress Card No.: applied forA catalogue record for this book is available from the British Library.Bibliographic information published by Die Deutsche Bibliothek Die Deutsche Bibliothek lists this publication in the Deutsche Nationalbibliografie; detailed bibliographic data is available in the Internet at http://dnb.ddb.de

The interaction between atrazine and the mineral horizon of soil: a spectroscopic study

“…Amorphous silica has a weak Bronsted acidity due to the silanol groups. At higher temperatures under laboratory conditions, silica can develop Lewis acidity and basicity due to the activation of siloxane groups during the course of dehydration (Grun et al 1996, Kromidas 2005.…”

The Role of and the Place of Method Validation in Drug Analysis Using Electroanalytical Techniques

“…The selected reference (or pharmacopoeia) method can in principle be used to test for bias in another method under validation. If the proposed electroanalytical method and the reference method agree, this is good evidence they both work for the studied compound and application media such dosage forms, biological material etc [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][59][60][61][62]. For obtaining the accuracy from these results, student-t and F tests are generally used for statistical comparison methods [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][59][60][61][62].…”

“…Then, when the correlation coefficient values reach at least 0.999 the linearity can be assumed in the concentration range for which standard solutions had been prepared during the calibration procedure. However, this parameter is mostly used, but correlation coefficient (r) is neither a proof of linearity nor suitable for quantitative measurement [19,25,[48][49][50][51][52]. Correlation coefficient (r) just indicates the extent of the relationship between the dependent and independent variables such as the electroanalytical response and the electroactive compound concentration.…”

“…The difference between the spiked and unspiked sample results is the recovered part of the added analyte, which can be compared with the known amount added. In general, for standard addition using this technique, a good approach is to add 25, 50 and 100% of the expected working compound concentration to the matrix in different experiments [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][59][60][61][62]. The measured amounts are reported versus the amount added with the average percent recovery results, RSD% and bias% results.…”

Development of Quality Control Method for Glucofarmaka Antidiabetic Jamu by HPLC Fingerprint Analysis

“…It is expected that those peaks come from compound components which did not retain in the column. According to literature, the peaks with a retention time of 2 min or less is expected to come from unretained compounds [16]. Thus, the resolution was not affected by the percentage of the organic modifier.…”