More Flexibility in Exchange Rates—And in Methods

Schmitz, Wolfgang

doi:10.20955/r.52.11-16.gbf

Cited by 3 publications

(9 citation statements)

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“…formate dehydrogenase from Clostridium thennaaceticum [18], Ctoatridium famzicoaeetieum [19] and Metha,ococcus vanni¢/li [20], ¢arboxylic acia rcduetase from C. thermoaceticum [21] and C. formF coaceticum [22], aldehyde:ferredoxin oxidoreduetase from Pyracoccgs furiosus [23] and the tungsten formylmet hanofuran dehydrogenase from hi. walfei [7]. rite oxidase [14], and the tungsten enzyme, formate dchydrogenase, from C themloaceticum [24], The EPR signals of these enzymes were much more complex than the signal displayed by the tungsten.s~bstituted fonnylm¢thanofuran dehydrogenas~ which is cl~rly derived from tungsten, as evidenced by characteristic ~=~W isotope splitting.…”

Section: Discussionmentioning

confidence: 99%

“…ins molybdate and supplemented with tun~tat¢ (l /aM) [7]. The formylmethanofuran dchydrogcna~ was purified from thee cells by fast protein liquid chromatollraphy (FPLC) as described prcviomdy [TJ, The FPLC columns were from Phannacia (Fr¢ibuql, Germany).…”

Section: Me#laobartcril# W~[ei (Dsm 2910) Was Grown On Medium Lack-mentioning

confidence: 99%

“…The formylmethanofuran dchydrogcna~ was purified from thee cells by fast protein liquid chromatollraphy (FPLC) as described prcviomdy [TJ, The FPLC columns were from Phannacia (Fr¢ibuql, Germany). Formylmcthanofuran dehydrolicnam= activity was ar~yed with methylviologen as electron acceptor [$] at 65"C in SO mM Tris-HCl, pH ?,3, Formylmethanofuran was synth~ized front methan~ruran and 4-nitrophenyl formate [9], Molybdenum and tungsten were determined via inductively coupled plasma mass sixctrometry [7].…”

Section: Me#laobartcril# W~[ei (Dsm 2910) Was Grown On Medium Lack-mentioning

confidence: 99%

“…Recently it was found that Methanobacterium wotfei can grow equally well on medium supplemented with tungstate instead of molybdatc [7]. During growth in the presen~ of tungstate (I /aM) a second formylethanofuran dehydrogenase was expressed, which contained tungsten bound to molybdopterin guanine dinucleotide.…”

Section: Introductionmentioning

confidence: 99%

“…During growth in the presen~ of tungstate (I /aM) a second formylethanofuran dehydrogenase was expressed, which contained tungsten bound to molybdopterin guanine dinucleotide. This tungsten formylmethanofuran dehydrogenase differed significantly from the molybdenum formylmethanofuran dehydrog~nase in chromatographic behaviour and catalytic properties [4,7].…”

Section: Introductionmentioning

confidence: 99%

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Properties of the tungsten‐substituted molybdenum formylmethanofuran dehydrogenase from Methanobacterium wolfei

1992

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Rcc*ivcd IS ,Iuly I992In Methcmotmctrrilun wo~¢i two formylmethanofuran dchydrolcnasmi arc present, one of which is a molybdenum-and the other a t unilstcn enwme, Wc report here that also the 'molybdenum' enzyme contained tunpten when the archaeon was lrown on molybdenum.deprived medium supplemented with tunlpitat¢ (I #M), Uncxi~.¢tcdly the tunlt=ten-substituted molybdenum enzyme was catalytically active and displayed a rhombic EPR sisnal which was attributed to run.ten by the characteristic ~'~W splitting, Fomaylmethanofuran dehydro~tnasc: TunBsten.substituted molybdenum protein: TunlP~ten EPR signal; Mcthanolenic archaea

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Section: Discussionmentioning

confidence: 99%

Section: Me#laobartcril# W~[ei (Dsm 2910) Was Grown On Medium Lack-mentioning

confidence: 99%

Section: Me#laobartcril# W~[ei (Dsm 2910) Was Grown On Medium Lack-mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Properties of the tungsten‐substituted molybdenum formylmethanofuran dehydrogenase from Methanobacterium wolfei

1992

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The influence of full leg-length compression tights during treadmill running at race speed

McManus

Butson

Rogerson

et al. 2022

International Journal of Sports Science & Coaching

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The aim of the present study was to examine whether full leg-length compression tights modify physiological and kinematic measures during treadmill running at a competitive race pace in moderately trained runners. Thirteen males and five females completed two 15-minute running tests at a speed corresponding to a recent race time wearing compression tights or loose-fitting running shorts. Running economy (RE) was determined by oxygen consumption and carbon dioxide expiration during the final 3 minutes of treadmill running. Muscle oxygenation, skin temperature, heart rate (HR), vertical oscillation, step frequency and ground contact time (GCT) were measured continuously. GCT was shorter with compression compared with control trials ( p = 0.03), however, no differences in RE, muscle oxygenation, vertical oscillation, step frequency, HR or skin temperature were revealed. Despite a shorter GCT with compression tights, the findings suggest that moderately trained runners do not benefit nor limit physiological responses at a competitive race pace.

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Prediction of Pneumonia 30-Day Readmissions: A Single-Center Attempt to Increase Model Performance

Mather¹,

Fortunato²,

Ash³

et al. 2013

Respir Care

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BACKGROUND: Existing models developed to predict 30 days readmissions for pneumonia lack discriminative ability. We attempted to increase model performance with the addition of variables found to be of benefit in other studies. METHODS: From 133,368 admissions to a tertiary-care hospital from January 2009 to March 2012, the study cohort consisted of 956 index admissions for pneumonia, using the Centers for Medicare and Medicaid Services definition. We collected variables previously reported to be associated with 30-day all-cause readmission, including vital signs, comorbidities, laboratory values, demographics, socioeconomic indicators, and indicators of hospital utilization. Separate logistic regression models were developed to identify the predictors of all-cause hospital readmission 30 days after discharge from the index pneumonia admission for pneumonia-related readmissions, and for pneumonia-unrelated readmissions. RESULTS: Of the 965 index admissions for pneumonia, 148 (15.5%) subjects were readmitted within 30 days. The variables in the multivariate-model that were significantly associated with 30-day all-cause readmission were male sex (odds ratio 1.59, 95% CI 1.03-2.45), 3 or more previous admissions (odds ratio 1.84, 95% CI 1.22-2.78), chronic lung disease (odds ratio 1.63, 95% CI 1.07-2.48), cancer (odds ratio 2.18, 95% CI 1.24 -3.84), median income < $43,000 (odds ratio 1.82, 95% CI 1.18 -2.81), history of anxiety or depression (odds ratio 1.62, 95% CI 1.04 -2.52), and hematocrit < 30% (odds ratio 1.86, 95% CI 1.07-3.22). The model performance, as measured by the C statistic, was 0.71 (0.66 -0.75), with minimal optimism according to bootstrap re-sampling (optimism corrected C statistic 0.67). CONCLUSIONS: The addition of socioeconomic status and healthcare utilization variables significantly improved model performance, compared to the model using only the Centers for Medicare and Medicaid Services variables.

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More Flexibility in Exchange Rates—And in Methods

Cited by 3 publications

References 0 publications

Properties of the tungsten‐substituted molybdenum formylmethanofuran dehydrogenase from Methanobacterium wolfei

Properties of the tungsten‐substituted molybdenum formylmethanofuran dehydrogenase from Methanobacterium wolfei

The influence of full leg-length compression tights during treadmill running at race speed

Prediction of Pneumonia 30-Day Readmissions: A Single-Center Attempt to Increase Model Performance

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