More expressions of BDNF and TrkB in multiple hepatocellular carcinoma and anti-BDNF or K252a induced apoptosis, supressed invasion of HepG2 and HCCLM3 cells

Guo, De‐An; Hou, Xuezhong; Zhang, Hongbin; Sun, Wenyu; Zhu, Lei; Liang, Jian; Jiang, Xiaofeng

doi:10.1186/1756-9966-30-97

Cited by 43 publications

(37 citation statements)

References 26 publications

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“…All products were purchased from SunBio Biotech, Co., Ltd. (Beijing, China). Lenti-miR-10a-5p or lenti-miRC [100 nM; GenScript (Nanjing) Co., Ltd., Nanjing, China] were then transduced into HeLa and SiHa cells using Lipofectamine 2000 (Biomics Biotechnologies Co., Ltd., Nantong, China) following a previously published protocol (18). HeLa and SiHa cells were selected from the five cell lines studied to determine miR-10-5p expression levels.…”

Section: Rna Extraction and Reverse Transcription-quantitative Polymementioning

confidence: 99%

MicroRNA‑10a‑5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF

Zhai¹,

Li²,

Lan³

et al. 2017

Exp Ther Med

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Abstract. The aim of the present study was to investigate the effect and mechanism of microRNA (miR)-10a-5p in human cervical cancer. The expression level of miR-10-5p in cervical cancer lines was assessed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). In cervical cancer HeLa and SiHa cells, miR-10-5p was ectopically overexpressed by lentiviral transduction. Brain-derived neurotrophic factor (BDNF) was then overexpressed in HeLa and SiHa cells to evaluate its selective effect on miR-10-5p in cervical cancer modulation. The targeting of miR-

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Section: Rna Extraction and Reverse Transcription-quantitative Polymementioning

confidence: 99%

MicroRNA‑10a‑5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF

Zhai¹,

Li²,

Lan³

et al. 2017

Exp Ther Med

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“…Tumorigenicity is a concern with all growth factors, and BDNF has been associated with tumour promotion, poor prognosis and more invasive disease across a wide range of malignancies, including squamous cell-, colorectal-, hepatocellular-, breastand cervix cancer as well as neuroendocrine tumours [63][64][65][66][67][68][69][70]. Tumour promotion with other growth factors used topically has been de-scribed and can occur also at sites distal from the site of administration.…”

Section: Rationale For Bdnf In Poagmentioning

confidence: 99%

Role of Brain-Derived Neurotrophic Factor for the Treatment of Glaucoma and Retinitis Pigmentosa

Spaccapelo¹

2016

MOJT

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Primary open-angle glaucoma (POAG) is the most common form of glau¬co¬ma, representing up to 90% of cases. POAG is described as a multifactorial optic neuropathy that is chronic and progressive with a characteristic acquired loss of optic nerve fibres. The cause for the elevated is generally IOP accepted to be decreased facility of aqueous outflow through the trabecular meshwork. Retinitis pigmentosa (RP) is a group of relatively rare, inherited disorders characterized by progressive peripheral vision loss and night vision difficulties (nyctalopia) that can lead to central vision loss due to the degeneration of the rod photoreceptor cells in the retina. RP manifests initial symptoms in-dependent of age; thus, RP diagnosis occurs anywhere from early infancy to late adulthood. Brain-derived neurotrophic factor (BDNF) is a 14 kDa protein belonging to the neurotrophin family of growth factors. BDNF has trophic functions in the hippocampus, cortex, and basal forebrain as well as the retina, motor neurons, the kidneys, saliva, and the prostate. The notion that BDNF may have a role in treating the neurodegenerative aspects of POAG is underpinned by convincing preclinical evidence in animal models of glaucoma that BDNF administered intravitreously can, at least in part, rescue retinal ganglion cells (RGCs) under conditions such as those induced by increased intraocular pressure(IOP). Overall, and despite a mechanistically sound rationale, the pharmacokinetic challenges indicate that instilled BDNF topical administration is associated with higher than normal risks, likely explaining the emphasis on alternative approaches (such as synthetic small molecule BDNF analogues or gene therapy approaches) in the last decade. Moreover, the high BDNF doses required to drive efficacy (and low target tissue penetration) also raise concerns over promotion of tumour growth resulting from BDNF taken up from non-retinal tissues. In conclusion, even if preliminary evidences are available, further studies are necessary in order to clarify the role of BDNF for the treatment of glaucoma and retinitis pigmentosa. Keywords:

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“…The increase in the expression of BDNF and its receptors in cells has been demonstrated by clinical studies generally in pathological conditions or after damage 11,14,15,20 .…”

Section: Introductionmentioning

confidence: 99%

“…Two types of BDNF receptors have been identified: activation of the nerve growth factor receptor p75 (NGFR p75) receptors leads to apoptosis, while activation of tropomyosinrelated kinase B (TrkB) receptors leads to cellular proliferation and differentiation 15,22,23 . Studies have shown that BDNF has high affinity for TrkB receptors, whereas it has low affinity for NGFR p75 receptors 24 .…”

Section: Introductionmentioning

confidence: 99%

“…BDNF expression was primarily detected in the hippocampus, cortex, and basal forebrain, areas where the brain performs learning, memory, and high-level thinking tasks 13 . Later, it was found that BDNF was presented not only in the brain but also in peripheral tissues such as skeletal muscle, adipose tissue, kidneys, liver and prostate 5,10,11,[14][15][16][17][18][19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

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Apoptotic Effects of Reduced Brain Derived Neurotrophic Factor (BDNF) on Mouse Liver and Kidney

Tezcan¹,

Hacioglu²,

Abidin³

et al. 2017

Dicle Tıp Dergisi

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Objective: Brain-derived neurotrophic factor (BDNF) promotes the development and differentiation of neurons and synapses, as well as neuronal survival, by acting on specific neuronal groups in the central and peripheral nervous systems. However, the direct effect of BDNF on apoptosis in peripheral tissues is not known. The aim of this study was to investigate the relationship between BDNF and apoptosis, and the density and distribution of BDNF receptors in liver and kidney tissues by histological and immunehistochemical methods.Methods: Seven wild-type and 7 BDNF heterozygous (reduced BDNF levels) male mice were used in the study. Caspase-3 and TUNEL immunehistochemical stainings were performed in order to investigate the presence of apoptosis in the liver and kidney tissues of the studied groups. Apoptosis-entering cells were counted and the groups were compared. Concentration and distribution of BDNF receptors, tropomyosin-related kinase B (TrkB) and nerve growth factor receptor p75 (NGFR p75), in liver and kidney tissues were also examined by immunehistochemical analyzes.Results: As a result of Caspase-3 and TUNEL immunehistochemical staining, more cells were counted to enter the apoptotic process in sections of BDNF heterozygous group compared to control group (p<0.0001). In both groups TrkB and NGFR p75 receptors in liver and kidney tissues were determined in trace amounts, but there was no difference in intensity and distribution between the studied groups.Conclusion: According to our histological and immunehistochemical stainings and statistical analysis of cell count between groups, it was found that BDNF is protective against apoptosis in liver and kidney. The lack of difference between the studied groups in terms of intensity and distribution of BDNF receptors, suggests that BDNF receptor distribution in the liver and kidney tissues may be different from the nervous system or that BDNF may differ in affinity for these receptors. Keywords 316 Azaltılmış Beyin Kaynaklı Nörotrofik Faktörün (BDNF), Fare Karaciğer ve Böbrek Dokusuna Apoptotik EtkileriÖzet Amaç: Beyin kökenli nörotrofik faktör (BDNF), merkezi ve periferik sinir sisteminde belirli nöron grupları üzerine etki ederek, nöron ve sinapsların gelişimini ve farklılaşmasını, bunun yanında nöronal sağkalımı desteklemektedir. Periferik dokularda ise BDNF'nin apoptoz ile direkt ilişkisi bilinmemektedir. Bu çalışmada, karaciğer ve böbrek dokularında BDNF'nin apoptoz ile ilişkisi ve bu dokulardaki BDNF reseptörlerinin yoğunluk ve dağılımının histolojik ve immünohistokimyasal yöntemler kullanılarak araştırılması amaçlanmıştır.Yöntemler: Araştırmada 7 adet yabanıl tip ve 7 adet BDNF heterozigot (BDNF düzeyleri yarı yarıya azaltılmış) erkek fare kullanılmıştır. Karaciğer ve böbrek dokusunda apoptozun varlığını araştırmak amacıyla Kaspaz-3 ve TUNEL immünohistokimyasal boyamaları gerçekleştirilmiş ve apoptoza giren hücreler sayılarak gruplar arası karşılaştırma yapılmıştır. BDNF reseptörleri olan tropomiyozin-ilişkili kinaz B (TrkB) ve nerve growth fa...

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More expressions of BDNF and TrkB in multiple hepatocellular carcinoma and anti-BDNF or K252a induced apoptosis, supressed invasion of HepG2 and HCCLM3 cells

Cited by 43 publications

References 26 publications

MicroRNA‑10a‑5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF

MicroRNA‑10a‑5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF

Role of Brain-Derived Neurotrophic Factor for the Treatment of Glaucoma and Retinitis Pigmentosa

Apoptotic Effects of Reduced Brain Derived Neurotrophic Factor (BDNF) on Mouse Liver and Kidney

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