MoRBP9 Encoding a Ran-Binding Protein Microtubule-Organizing Center Is Required for Asexual Reproduction and Infection in the Rice Blast Pathogen Magnaporthe oryzae

Fu, Teng; Park, Gi-Chang; Han, Joon Hee; Shin, Jung Won; Park, Hyun-Hoo; Kim, Kyoung Su

doi:10.5423/ppj.oa.07.2019.0204

Cited by 8 publications

(9 citation statements)

References 50 publications

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“…[38][39][40] In plant pathogenic fungi, the loss of MoRBP9 and CsBzip10 also cause a decrease in the rate of appressoria formation and a reduction in the pathogenicity of the mutant strains. 41,42 Recently, CreA has been shown to participate in the formation of appressoria, the KMT2-Cre1-Hyd4 regulatory pathway regulates appressoria and pathogenicity in M. robertsii 27 and this pathway is crucial for appressorial formation in Metarhizium and the lack of Cre1 leads to the block of appressorium formation in this pathway, which is similar to SET1-Cre1-Hyd1/2 pathway in B. bassiana. 43 Lipids in appressoria are degraded into glycerol to provide pressure for mechanical penetration, 6 therefore the turgor pressure of the appressoria is important for fungal invasion.…”

Section: Discussionmentioning

confidence: 90%

The carbon catabolite repressor CreA is an essential virulence factor of Metarhizium acridum against Locusta migratoria

Song

Jin

Shi

et al. 2022

Pest Management Science

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BACKGROUND: CreA has been proved to be a core gene in asexual conidiation in Metarhizium acridum, which regulates the shift of normal conidiation and microcycle conidiation. At present, research on CreA in fungi has focused on carbon source metabolism. There is a lack of research on the effect of CreA in virulence of pathogenic fungi.RESULTS: The virulence of the MaCreA disrupted strain (ΔMaCreA) for Locusta migratoria was lost by topical inoculation bioassay. The formation rate and turgor pressure of the appressoria decreased. Growth of ΔMaCreA in host hemolymph was delayed, and the number of hyphal bodies was significantly reduced. The conidial cell wall of ΔMaCreA became thicker, the mannan content decreased, and the chitin content increased significantly, and it was more sensitive to calcofluor white and Congo Red. ⊍-1,3-Glucan and ⊎-1,3-glucan are more exposed on the surface of ΔMaCreA conidia than on the wild type. Lmspätzle and Lmcactus, the immune response genes in the host Toll pathway, showed stronger transcriptional activities at the early stage of ΔMaCreA invasion. The phenoloxidase activity assay also showed stronger immunostimulation by ΔMaCreA in vitro. CONCLUSION:The main reasons for the loss of virulence of ΔMaCreA in the topical inoculation were the reduced penetration ability of appressoria, limited growth in hemolymph and stronger insect immunostimulation of ΔMaCreA.

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Section: Discussionmentioning

confidence: 90%

The carbon catabolite repressor CreA is an essential virulence factor of Metarhizium acridum against Locusta migratoria

Song

Jin

Shi

et al. 2022

Pest Management Science

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“…Using the InterPro term IPR003527 (MAP kinase and conserved site) as a reference to search the genome of C. scovillei , three genetic loci were identified as: CAP_011033 , CAP_001359 , and CAP_009200 . 1 The phylogenetic relationship of MAPKs in C. scovillei with their orthologs in other fungi was analyzed using the maximum-likelihood method with 1,000 bootstraps in the MEGA 7 software ( Fu et al, 2019 ). Targeted deletion of CsPMK1 was performed by using a modified double-joint PCR ( Yu et al, 2004 ).…”

Section: Methodsmentioning

confidence: 99%

Mitogen-Activated Protein Kinase CsPMK1 Is Essential for Pepper Fruit Anthracnose by Colletotrichum scovillei

Shin

Lee

et al. 2022

Front. Microbiol.

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The phytopathogenic fungus Colletotrichum scovillei, belonging to the Colletotrichum acutatum species complex, causes severe anthracnose disease on several fruits, including chili pepper (Capsicum annuum). However, the molecular mechanisms underlying the development and pathogenicity of Colletotrichum scovillei are unclear. The conserved Fus3/Kss1-related MAPK regulates fungal development and pathogenicity. Here, the role of CsPMK1, orthologous to Fus3/Kss1, was characterized by phenotypic comparison of a target deletion mutant (ΔCspmk1). The mycelial growth and conidiation of ΔCspmk1 were normal compared to that of the wild type. ΔCspmk1 produced morphologically abnormal conidia, which were delayed in conidial germination. Germinated conidia of ΔCspmk1 failed to develop appressoria on inductive surfaces of hydrophobic coverslips and host plants. ΔCspmk1 was completely defective in infectious growth, which may result from failure to suppress host immunity. Furthermore, ΔCspmk1 was impaired in nuclear division and lipid mobilization during appressorium formation, in response to a hydrophobic surface. CsPMK1 was found to interact with CsHOX7, a homeobox transcription factor essential for appressorium formation, via a yeast two-hybridization analysis. Taken together, these findings suggest that CsPMK1 is required for fungal development, stress adaptation, and pathogenicity of C. scovillei.

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“…Conidiation was evaluated by counting conidia collected with 5 ml of distilled water from 7-day-old V8 agar. Lactophenol aniline blue solution was used to distinguish conidiophores from mycelia ( 33 , 92 ). For conidial germination and appressorium formation, drops of conidial suspension (20 μl; 5 × 10 4 ml −1 ) were placed on hydrophobic coverslips, then incubated in a humid box.…”

Section: Methodsmentioning

confidence: 99%

Homeobox Transcription Factors Are Required for Fungal Development and the Suppression of Host Defense Mechanisms in the Colletotrichum scovillei -Pepper Pathosystem

Han

Shin

et al. 2021

mBio

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MoRBP9 Encoding a Ran-Binding Protein Microtubule-Organizing Center Is Required for Asexual Reproduction and Infection in the Rice Blast Pathogen Magnaporthe oryzae

Cited by 8 publications

References 50 publications

The carbon catabolite repressor CreA is an essential virulence factor of Metarhizium acridum against Locusta migratoria

The carbon catabolite repressor CreA is an essential virulence factor of Metarhizium acridum against Locusta migratoria

Mitogen-Activated Protein Kinase CsPMK1 Is Essential for Pepper Fruit Anthracnose by Colletotrichum scovillei

Homeobox Transcription Factors Are Required for Fungal Development and the Suppression of Host Defense Mechanisms in the Colletotrichum scovillei -Pepper Pathosystem

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