Moraea sisyrinchium inhibits proliferation, cell cycle, and migration of cancerous cells and decreases angiogenesis in chick chorioallantoic membrane

Abstract

Objective Experimental studies reported that some plants in the genus of Moraea (Iridaceae family) show anticancer multiform and HepG2 liver cancer cells. Methods The cells were incubated potential. This study aimed to evaluate the effects of Moraea sisyrinchium on U87 Glioblastoma for 24 with hydro alcoholic extract of the stem, flower, and bulb of Moraea sisyrinchium. The cell proliferation, cell cycle, and migration were determined by MTT assay, propidium iodide staining, and scratch assay, respectively. Oxidative stress was evaluated by determining the levels of reactive oxygen species and lipid peroxidation. The activity of matrix metalloproteinases (MMPs), the expression of Bax and Bcl-2 proteins, and angiogenesis were evaluated by the gelatin zymography, Western blotting, and model of chick embryo chorioallantoic membrane, respectively. Results The extracts of the flower, stem, and bulb significantly decreased the proliferation of HepG2 and U87 cells. This effect was more for U87 than for HepG2 and for bulb and stem than for flower. In U87 cells, the bulb extract increased oxidative stress, cell cycle arrest, and the Bax/Bcl-2 ratio. Also, this extract suppressed the migration ability of HepG2 and U87 cells, which was associated with the inhibition of MMP2 activity. In addition, it significantly reduced the number and diameter of vessels in the chorioallantoic membrane. Liquid chromatography-mass spectrometry revealed the presence of xanthones (bellidifolin, mangiferin), flavonoids (quercetin, luteolin), isoflavones (iridin,tectorigenin),and phytosterols (e.g., stigmasterol) in the bulb extract. Conclusion The extract of Moraea sisyrinchium bulb decreased the proliferation and survival of cancer cells by inducing oxidative stress. The extract also reduced the migration ability of the cells and inhibited angiogenesis.