Mopeia Virus–related Arenavirus in Natal Multimammate Mice<i>,</i>Morogoro, Tanzania

Günther, Stephan; Hoofd, G.; Charrel, Rémi N.; Röser, Christina; Becker-Ziaja, Beate; Lloyd, Graham; Sabuni, Christopher; Verhagen, Ron; Groen, G van der; Kennis, Jan; Katakweba, Abdul; Machang’u, Robert S.; Makundi, Rhodes H.; Leirs, Herwig

doi:10.3201/eid1512.090864

Cited by 59 publications

(67 citation statements)

References 13 publications

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“…Lassa virus was used as an antigen due to its known cross-reactivity with immune sera from animals infected with other OWAs [3], [6], [18]. While the remaining three Hylomyscus sp.…”

Section: Resultsmentioning

confidence: 99%

Novel Arenavirus Sequences in Hylomyscus sp. and Mus (Nannomys) setulosus from Côte d'Ivoire: Implications for Evolution of Arenaviruses in Africa

et al. 2011

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This study aimed to identify new arenaviruses and gather insights in the evolution of arenaviruses in Africa. During 2003 through 2005, 1,228 small mammals representing 14 different genera were trapped in 9 villages in south, east, and middle west of Côte d'Ivoire. Specimens were screened by pan-Old World arenavirus RT-PCRs targeting S and L RNA segments as well as immunofluorescence assay. Sequences of two novel tentative species of the family Arenaviridae, Menekre and Gbagroube virus, were detected in Hylomyscus sp. and Mus (Nannomys) setulosus, respectively. Arenavirus infection of Mus (Nannomys) setulosus was also demonstrated by serological testing. Lassa virus was not found, although 60% of the captured animals were Mastomys natalensis. Complete S RNA and partial L RNA sequences of the novel viruses were recovered from the rodent specimens and subjected to phylogenetic analysis. Gbagroube virus is a closely related sister taxon of Lassa virus, while Menekre virus clusters with the Ippy/Mobala/Mopeia virus complex. Reconstruction of possible virus–host co-phylogeny scenarios suggests that, within the African continent, signatures of co-evolution might have been obliterated by multiple host-switching events.

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“…Lassa virus was used as an antigen due to its known cross-reactivity with immune sera from animals infected with other OWAs [3], [6], [18]. While the remaining three Hylomyscus sp.…”

Section: Resultsmentioning

confidence: 99%

Novel Arenavirus Sequences in Hylomyscus sp. and Mus (Nannomys) setulosus from Côte d'Ivoire: Implications for Evolution of Arenaviruses in Africa

et al. 2011

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“…At least 10 new arenaviruses have been discovered in the last 3 years as a result of both rodent surveys and disease outbreaks (Briese et al, 2009; Cajimat et al, 2007; 2008; Delgado et al, 2008; Gunther et al, 2009; Lecompte et al, 2007; Milazzo et al, 2008; Palacios et al, 2008; 2010; Vieth et al, 2007), but not all of the new viruses qualify as distinct arenavirus species (Salvato et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Lymphocytic choriomeningitis virus (LCMV) infection of macaques: A model for Lassa fever

et al. 2011

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Arenaviruses such as Lassa fever virus (LASV) and lymphocytic choriomeningitis virus (LCMV) are benign in their natural reservoir hosts, and can occasionally cause severe viral hemorrhagic fever (VHF) in non-human primates and in human beings. LCMV is considerably more benign for human beings than Lassa virus, however certain strains, like the LCMV-WE strain, can cause severe disease when the virus is delivered as a high-dose inoculum. Here we describe a rhesus macaque model for Lassa fever that employs a virulent strain of LCMV. Since LASV must be studied within Biosafety Level-4 (BSL-4) facilities, the LCMV-infected macaque model has the advantage that it can be used at BSL-3. LCMV-induced disease is rarely as severe as other VHF, but it is similar in cases where vascular leakage leads to lethal systemic failure. The LCMV-infected macaque has been valuable for describing the course of disease with differing viral strains, doses and routes of infection. By monitoring system-wide changes in physiology and gene expression in a controlled experimental setting, it is possible to identify events that are pathognomonic for developing VHF and potential treatment targets.

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“…To identify primers and probes, conserved regions specific to LCMV were screened based on an extensive alignment of all LCMV and Old World arenavirus's sequences available in Genbank in August 2010, including all recently discovered arenaviruses (Briese et al, 2009;Gunther et al, 2009;Palacios et al, 2008). This alignment allowed to pinpoint a conserved region within the viral nucleoprotein (NP) gene.…”

Section: Introductionmentioning

confidence: 99%

Analytical validation of a lymphocytic choriomeningitis virus real-time RT-PCR assay

Cordey

Sahli

Moraz

et al. 2011

Journal of Virological Methods

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Mopeia Virus–related Arenavirus in Natal Multimammate Mice,Morogoro, Tanzania

Cited by 59 publications

References 13 publications

Novel Arenavirus Sequences in Hylomyscus sp. and Mus (Nannomys) setulosus from Côte d'Ivoire: Implications for Evolution of Arenaviruses in Africa

Novel Arenavirus Sequences in Hylomyscus sp. and Mus (Nannomys) setulosus from Côte d'Ivoire: Implications for Evolution of Arenaviruses in Africa

Lymphocytic choriomeningitis virus (LCMV) infection of macaques: A model for Lassa fever

Analytical validation of a lymphocytic choriomeningitis virus real-time RT-PCR assay

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