Monthly variations in ovine seminal plasma proteins analyzed by two-dimensional polyacrylamide gel electrophoresis

Cardozo, J.A.; Fernández‐Juan, Marta; Forcada, F.; Abecia, J.A.; Muiño‐Blanco, T.; Cebrián‐Pérez, J. A.

doi:10.1016/j.theriogenology.2006.01.058

Cited by 80 publications

(64 citation statements)

References 44 publications

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“…The study conducted by Yue et al (2009), protein spots with a molecular weight from 15-20 kDa accounted for 41% of the relative intensity of the spots of the gel. Some authors observed that when the molecular weights range between 12.5 and 83.9 kDa, the protein spots <21 kDa had the highest relative intensity using the gradient gel (Cardozo et al, 2006). Available reports reveals that proteins below 45 kDa, but in our study it was less than 40 kDa.…”

Section: Resultscontrasting

confidence: 41%

Analysis of Seminal Plasma Proteins of South Indian Jersey and Hybrid Bulls and their Correlation with Semen Quality

Vickram¹,

Rajeswari²,

Pathy³

et al. 2015

Asian J. of Animal Sciences

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This is a preliminary study of the protein profiles of the semen in Jersey breed bulls. Jersey bulls were mainly used for fertility purposes; they were grouped based on the fertility, such as highly fertile group (95% fertility), medium fertile group (65-70%), low fertile group (45-50%) and hybrid bulls. Semen of 20 Jersey and hybrid bulls was collected through artificial vagina. The semen characteristics includes volume, pH value, viscosity, viability, sperm concentration, agglutination, total motility and their group, sperm morphology and Hypo-Osmotic Swelling test (HOS) were carried out immediately after collection. Total antioxidant capacity test by using catalase and cholesterol analysis were also carried out. Quantification of the amino acids and proteins were carried out by Ninhydrin and Lowry method, protein analysis by Sodium Dodecyl Sulphate (SDS) was carried out for the following categories. (1) Highly fertile group (Jersey), (2) Medium fertile group (Jersey), (3) Low fertile group (Jersey) and (4) Hybrid group. Analysis of semen protein reveals about seventeen protein bands of sizes ranging between 14 and 205 kDa were identified. Among those proteins bands, the relative protein content of nine bands shows significantly different from each group. The rest of the proteins bands seem to have some positive and/or negative correlation on the semen characterizes or fertility. The antioxidant capacity of highly fertile and hybrid group was very high compare to low fertile group of Jersey. This study clearly indicates the bull seminal plasma proteins are associated with fertility and as well as determination of semen quality and ultimately decides the fertility.Key words: Semen, seminal plasma, jersey bulls, catalase, antioxidants, ninhydrin test INTRODUCTIONSemen consists of mature spermatozoon and swims in a highly complex features seminal fluid. Seminal fluid contains a complex mixture of organic compounds secreted from epididymis, testes and other accessory sex glands, influences sperm morphology, motility, sperm concentration acrosome reaction and fertility (Mann and Lutwak-Mann, 1981). Biochemical analysis of seminal fluid reveals that it is composed of amino acids, proteins and polypeptides. These molecules of seminal plasma determines semen quality and fertility were different in different species, some are reported to be associated with the fertility. Good quality semen is the most important factor to implement breeding programs (Stradaioli et al., 2004).The presence of proteins, lipids and hormones in seminal plasma suggests that a large part of the physiological functions of this fluid are not yet ascertained. It is a fact that components especially, high levels of fructose and zinc are not present in other body fluids. Effective semen

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Section: Resultscontrasting

confidence: 41%

Analysis of Seminal Plasma Proteins of South Indian Jersey and Hybrid Bulls and their Correlation with Semen Quality

Vickram¹,

Rajeswari²,

Pathy³

et al. 2015

Asian J. of Animal Sciences

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“…Immediately before mixing with semen samples, 2.5% of egg yolk was added to this stock solution (Cardozo et al, 2006). After the final dilution, semen samples were incubated at 4ºC and frozen for 48 hours.…”

Section: Methodsmentioning

confidence: 99%

Sperm parameters and biochemical components of goat seminal plasma in the rainy and dry seasons in the Brazilian Northeast: the season's influence on the cooling of semen

Aguiar

Tilburg

Catunda

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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“…Seminal plasma has also been used to improve the limited fertility of frozen-thawed spermatozoa following cervical artificial insemination [11], yet contradictory reports of its success also exist [12][13][14]. This variability in effect observed within the literature is hypothesised to be attributed to differences in seminal plasma composition (namely proteins) between individual males [3,4,12,15] brought about by disparate physiological, nutritional and/or seasonal conditions [4,[15][16][17][18][19]. We have recently provided evidence to support this hypothesis and demonstrated that the source and composition of seminal plasma directly altered the ability of ram spermatozoa to survive cryopreservation [1].…”

Section: Introductionmentioning

confidence: 99%

“…For example, motility was increased when a 15, 19 and 80 kDa band was present in ram seminal plasma. Likewise, Cardozo et al [19] also compared the seminal plasma protein composition (2D-PAGE) to ram sperm motility over the course of a year. While such data provides encouraging evidence of the link between protein composition and freezing resilience, these proteins have yet to be identified or characterised via mass spectrometry.…”

Section: Introductionmentioning

confidence: 99%

The identification of proteomic markers of sperm freezing resilience in ram seminal plasma

Rickard

Leahy

Soleilhavoup

et al. 2015

Journal of Proteomics

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Monthly variations in ovine seminal plasma proteins analyzed by two-dimensional polyacrylamide gel electrophoresis

Cited by 80 publications

References 44 publications

Analysis of Seminal Plasma Proteins of South Indian Jersey and Hybrid Bulls and their Correlation with Semen Quality

Analysis of Seminal Plasma Proteins of South Indian Jersey and Hybrid Bulls and their Correlation with Semen Quality

Sperm parameters and biochemical components of goat seminal plasma in the rainy and dry seasons in the Brazilian Northeast: the season's influence on the cooling of semen

The identification of proteomic markers of sperm freezing resilience in ram seminal plasma

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