Montanide ISA™ 201 adjuvanted FMD vaccine induces improved immune responses and protection in cattle

Dar, Pervaiz Ahmad; Kalaivanan, Ramya; Sied, Nuru; Mamo, Bedaso; Kishore, Subodh; Suryanarayana, V.V.S.; Kondabattula, Ganesh

doi:10.1016/j.vaccine.2013.05.078

Cited by 79 publications

(50 citation statements)

References 23 publications

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“…In brief, LPS-free water reconstituted rSj97, or lyophilization buffer (50M sodium phosphate, 0.05% tween-20, 0.3% sucrose, pH7.4) as the control, was added to ISA206 at 50:50 (w:w) ratio. The mixtures were stirred at a low shear rate (300 rpm) for 10 min at 30°C in a glass beaker to form the water-in-oil-in-water emulsion [32]. …”

Section: Methodsmentioning

confidence: 99%

Vaccination with recombinant paramyosin in Montanide ISA206 protects against Schistosoma japonicum infection in water buffalo

et al. 2017

Vaccine

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Section: Methodsmentioning

confidence: 99%

Vaccination with recombinant paramyosin in Montanide ISA206 protects against Schistosoma japonicum infection in water buffalo

et al. 2017

Vaccine

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“…Three weeks post-immunization, PBMCs were separated from the blood of five randomly selected chickens per group as described previously [40]. In vitro proliferation of PBMCs to HA recall antigen was measured by MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] based assay as described in Dar et al [45]. …”

Section: Methodsmentioning

confidence: 99%

A consensus-hemagglutinin-based vaccine delivered by an attenuated Salmonella mutant protects chickens against heterologous H7N1 influenza virus

2017

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H7N3 and H7N7 are highly pathogenic avian influenza (HPAI) viruses and have posed a great threat not only for the poultry industry but for the human health as well. H7N9, a low pathogenic avian influenza (LPAI) virus, is also highly pathogenic to humans, and there is a great concern that these H7 subtypes would acquire the ability to spread efficiently between humans, thereby becoming a pandemic threat. A vaccine candidate covering all the three subtypes must, therefore, be an integral part of any pandemic preparedness plan. To address this need, we constructed a consensus hemagglutinin (HA) sequence of H7N3, H7N7, and H7N9 based on the data available in the NCBI in early 2012-2015. This artificial sequence was then optimized for protein expression before being transformed into an attenuated auxotrophic mutant of Salmonella Typhimurium, JOL1863 strain. Immunizing chickens with JOL1863, delivered intramuscularly, nasally or orally, elicited efficient humoral and cell mediated immune responses, independently of the route of vaccination. Our results also showed that JOL1863 deliver efficient maturation signals to chicken monocyte derived dendritic cells (MoDCs) which were characterized by upregulation of costimulatory molecules and higher cytokine induction. Moreover, immunization with JOL1863 in chickens conferred a significant protection against the heterologous LPAI H7N1 virus challenge as indicated by reduced viral sheddings in the cloacal swabs. We conclude that this vaccine, based on a consensus HA, could induce broader spectrum of protection against divergent H7 influenza viruses and thus warrants further study.

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“…As the most widely used commercial oil adjuvant, the ISA-206 is certainly more effective than conventional Al(OH) 3 /saponin-based adjuvant in terms of generating higher and longer lasting immune responses (Cao 2014). However, the less efficacy of ISA-206 in inducing cellmediated immune response and consequent insufficient protective efficacy in cattle against FMD virus had been frequently reported in recent years (Cao 2014, Dar et al 2013, Park et al 2014, Terhuja et al 2015. More recently, Terhuja et al compared efficacy of recombinant FMD virus-like particles (VLPs) adjuvant with ISA-206 and several of other chemical adjuvants including CpG (Terhuja et al 2015).…”

Section: Potential Adjuvant Effect Of Nano-emulsion Formulationmentioning

confidence: 99%

“…The SEPPIC Inc. has developed a newly mineral oil-based with commercial name of Montanide ISA-201 by adding some chemical components on the basis of ISA-206 to improve the cellular responses. Results from comparative studies had proven that as compared to ISA-206, the inactivated FMD vaccines formulated with the ISA-201 adjuvant could induce earlier and higher neutralizing antibody responses, and higher cellular immunity and better protective efficacy in cattle (Dar et al 2013). However, such improvement is based on addition of extra chemicals.…”

Section: Potential Adjuvant Effect Of Nano-emulsion Formulationmentioning

confidence: 99%

“…Currently, the Montanide TM ISA series of oil-adjuvants (SEPPIC, France) is used for formulation of FMD vaccines in Asian and South American countries. Montanide ISA-206 renders a water-in-oil-in-water double emulsion, and Montanide ISA-201 is another newly developed oil-based adjuvant retaining the advantage of ISA-206 and adding some chemical components on the basis of ISA-206 to improve the cellular response, producing higher immunity responses (Dar et al 2013).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Construction of a stable w/o nano-emulsion as a potential adjuvant for foot and mouth disease virus vaccine

Chen

Zhang

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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A stable and bio-compatible w/o nano-emulsion was developed as adjuvant for foot and mouth disease (FMD) vaccine with isopropyl myristate as oil phase, polyglycerol polyricinoleate, and Tween 80 as surfactants. Dissolving Tween 80 in aqueous phase decreased the droplet size and improved stability of the emulsion through narrowing size distribution, increasing viscosity, and reducing the interfacial tension between oil and water. The nano-emulsion was then formulated with inactivated FMD virus and ovalbumin to investigate its potential application as an adjuvant. Compared with commercial ISA-206 adjuvant, the novel w/o nano-emulsion was able to induce both humoral- and cell-mediated immune response.

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Montanide ISA™ 201 adjuvanted FMD vaccine induces improved immune responses and protection in cattle

Cited by 79 publications

References 23 publications

Vaccination with recombinant paramyosin in Montanide ISA206 protects against Schistosoma japonicum infection in water buffalo

Vaccination with recombinant paramyosin in Montanide ISA206 protects against Schistosoma japonicum infection in water buffalo

A consensus-hemagglutinin-based vaccine delivered by an attenuated Salmonella mutant protects chickens against heterologous H7N1 influenza virus

Construction of a stable w/o nano-emulsion as a potential adjuvant for foot and mouth disease virus vaccine

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