Monoubiquitination of Histone 2B at the Disease Resistance Gene Locus Regulates Its Expression and Impacts Immune Responses in Arabidopsis

Zou, Baohong; Yang, Donglei; Shi, Zhenying; Dong, Hansong; Hua, Jian

doi:10.1104/pp.113.227801

Cited by 82 publications

(94 citation statements)

References 57 publications

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“…SDG8 and SDG25 target genes, CER3 and CCR2, as well as SNC1 were used to determine the impact of H3Kme on H2Bubn. SNC1 is a TIR-NB-LRR-type protein whose gene expression is regulated by H2Bubn (Zou et al, 2014). As expected, the hub1 mutation significantly reduced H2Bubn in SNC1, CCR2, and CER3 promoters and exons ( Figures 9B to 9D).…”

Section: Crosstalk Between Histone Lysine Methylation and H2b Ubiquitsupporting

confidence: 48%

Global regulation of plant immunity by histone lysine methyl transferases

Lee

et al. 2016

Plant Cell

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Posttranslational modification of histones modulates gene expression affecting diverse biological functions. We showed that the Arabidopsis thaliana histone methyl transferases SET DOMAIN GROUP8 (SDG8) and SDG25 regulate pep1-, flg22-, and effector-triggered immunity as well as systemic acquired resistance. Genome-wide basal and induced transcriptome changes regulated by SDG8 and/or SDG25 showed that two genes of the SDG-dependent transcriptome, CAROTENOID ISOMERASE2 (CCR2) and ECERIFERUM3 (CER3), were also required for plant immunity, establishing mechanisms in defense functions for SDG8 and SDG25. CCR2 catalyzes the biosynthesis of carotenoids, whereas CER3 is involved in the biosynthesis of cuticular wax. SDG8 and SDG25 affected distinct and overlapping global and locus-specific histone H3 lysine 4 (H3K4) and histone H3 lysine 36 (H3K36) methylations. Loss of immunity in sdg mutants was attributed to altered global and CCR2-and CER3-specific histone lysine methylation (HLM). Loss of immunity in sdg, ccr2, and cer3 mutants was also associated with diminished accumulation of lipids and loss of cuticle integrity. In addition, sdg8 and sdg25 mutants were impaired in H2B ubiquitination (H2Bubn) at CCR2, CER3, and H2Bubn regulated R gene, SNC1, revealing crosstalk between the two types of histone modifications. In summary, SDG8 and SDG25 contribute to plant immunity directly through HLM or indirectly through H2Bubn and by regulating expression of plant immunity genes, accumulation of lipids, biosynthesis of carotenoids, and maintenance of cuticle integrity.

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Section: Crosstalk Between Histone Lysine Methylation and H2b Ubiquitsupporting

confidence: 48%

Global regulation of plant immunity by histone lysine methyl transferases

Lee

et al. 2016

Plant Cell

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“…H2Bub1 plays critical roles in regulating growth and development (Fleury et al ; Liu et al ; Cao et al ; Gu et al ; Schmitz et al ; Xu et al ; Bourbousse et al ; Himanen et al ; Cao et al ), as well as in modulating defence or immune responses and in cuticle composition in plants (Devoto et al ; Dhawan et al ; Hu et al ; Menard et al ; Zou et al ). In the present study, we demonstrated that H2Bub1 played an important role in response to salt stress.…”

Section: Discussionmentioning

confidence: 99%

Histone H2B monoubiquitination regulates salt stress‐induced microtubule depolymerization in Arabidopsis

Zhou

Chen

Sun

et al. 2017

Plant Cell & Environment

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Histone H2B monoubiquitination (H2Bub1) is recognized as a regulatory mechanism that controls a range of cellular processes. We previously showed that H2Bub1 was involved in responses to biotic stress in Arabidopsis. However, the molecular regulatory mechanisms of H2Bub1 in controlling responses to abiotic stress remain limited. Here, we report that HISTONE MONOUBIQUITINATION1 (HUB1) and HUB2 played important regulatory roles in response to salt stress. Phenotypic analysis revealed that H2Bub1 mutants confer decreased tolerance to salt stress. Further analysis showed that H2Bub1 regulated the depolymerization of microtubules (MTs), the expression of PROTEIN TYROSINE PHOSPHATASE1 (PTP1) and MAP KINASE PHOSPHATASE (MKP) genes - DsPTP1, MKP1, IBR5, PHS1, and was required for the activation of mitogen-activated protein kinase3 (MAP kinase3, MPK3) and MPK6 in response to salt stress. Moreover, both tyrosine phosphorylation and the activation of MPK3 and MPK6 affected MT stability in salt stress response. Thus, the results indicate that H2Bub1 regulates salt stress-induced MT depolymerization, and the PTP-MPK3/6 signalling module is responsible for integrating signalling pathways that regulate MT stability, which is critical for plant salt stress tolerance.

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“…In addition, H2Bub1 has been found to play a role in plant defense against pathogens. hub1 mutants show increased susceptibility to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola (Dhawan et al, 2009), and HUB1 and HUB2 have been shown to regulate the expression of R genes SUPPRESSOR OF npr1-1, CONSTITUTIVE1 and RESISTANCE TO PERONOSPORA PARASITICA4 via histone monoubiquitination at the R gene locus (Zou et al, 2014). Further, H2Bub1 mediated by HUB1/HUB2 is also involved in regulating the dynamics of microtubules to alter the defense response to Verticillium dahliae toxins in Arabidopsis (Hu et al, 2014).…”

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confidence: 99%

Histone H2B Monoubiquitination Mediated by HISTONE MONOUBIQUITINATION1 and HISTONE MONOUBIQUITINATION2 Is Involved in Anther Development by Regulating Tapetum Degradation-Related Genes in Rice

Cao

Wang

et al. 2015

Plant Physiology

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Histone H2B monoubiquitination (H2Bub1) is an important regulatory mechanism in eukaryotic gene transcription and is essential for normal plant development. However, the function of H2Bub1 in reproductive development remains elusive. Here, we report rice (Oryza sativa) HISTONE MONOUBIQUITINATION1 (OsHUB1) and OsHUB2, the homologs of Arabidopsis (Arabidopsis thaliana) HUB1 and HUB2 proteins, which function as E3 ligases in H2Bub1, are involved in late anther development in rice. oshub mutants exhibit abnormal tapetum development and aborted pollen in postmeiotic anthers. Knockout of OsHUB1 or OsHUB2 results in the loss of H2Bub1 and a reduction in the levels of dimethylated lysine-4 on histone 3 (H3K4me2). Anther transcriptome analysis revealed that several key tapetum degradation-related genes including OsC4, rice Cysteine Protease1 (OsCP1), and Undeveloped Tapetum1 (UDT1) were down-regulated in the mutants. Further, chromatin immunoprecipitation assays demonstrate that H2Bub1 directly targets OsC4, OsCP1, and UDT1 genes, and enrichment of H2Bub1 and H3K4me2 in the targets is consistent to some degree. Our studies suggest that histone H2B monoubiquitination, mediated by OsHUB1 and OsHUB2, is an important epigenetic modification that in concert with H3K4me2, modulates transcriptional regulation of anther development in rice.

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Monoubiquitination of Histone 2B at the Disease Resistance Gene Locus Regulates Its Expression and Impacts Immune Responses in Arabidopsis

Cited by 82 publications

References 57 publications

Global regulation of plant immunity by histone lysine methyl transferases

Global regulation of plant immunity by histone lysine methyl transferases

Histone H2B monoubiquitination regulates salt stress‐induced microtubule depolymerization in Arabidopsis

Histone H2B Monoubiquitination Mediated by HISTONE MONOUBIQUITINATION1 and HISTONE MONOUBIQUITINATION2 Is Involved in Anther Development by Regulating Tapetum Degradation-Related Genes in Rice

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