Monosaccharide inhibition of adherence by Pseudomonas aeruginosa to canine corneocytes

Abstract

The effect of D-galactose, D-mannose, L-rhamnose and dextrose on the adhesion to canine corneocytes by three strains of Pseudomonas aeruginosa was studied in six healthy dogs. Canine corneocytes were collected from the inner aspect of the pinna using adhesive discs (D-Squame). Half millimetre of bacterial suspension in phosphate-buffered saline (PBS) with or without the addition of a monosaccharide was placed over the corneocyte layer and incubated in moist chambers. Image analysis was used to quantify bacterial adherence to corneocytes. The three strains of Pseudomonas adhered well to canine corneocytes. All monosaccharides tested inhibited the adherence of Pseudomonas to canine corneocytes. The mean reduction in adhesion for individual sugars at a concentration of 0.1% was 40.2% (dextrose), 30.8% (L-rhamnose), 25.6% (D-galactose) and 19.4% (D-mannose). When D-galactose, D-mannose and L-rhamnose were used in combination at 0.1% concentration, the mean reduction in adherence was 52.9%. The monosaccharides studied may have a potential role in the management of Pseudomonas infections in dogs.

Reproducibility of a quantitative cutaneous cytological technique

“…It should also be noted that PMNs were not found in any of the OIFs from normal dogs. These IC rods may have been adhered to keratinocytes, as previously demonstrated using canine corneocyte cell cultures . Alternatively, these rods may have been melanin granules or stain precipitate associated with keratinocytes …”

“…These IC rods may have been adhered to keratinocytes, as previously demonstrated using canine corneocyte cell cultures. 18 Alternatively, these rods may have been melanin granules or stain precipitate associated with keratinocytes. 1,3 Given that PMNs were absent in all normal dogs, it is unknown why nuclear streaming was identified so frequently in these samples.…”

Inhibition of EGFR pathway signaling and the metastatic potential of breast cancer cells by PA-MSHA mediated by type 1 fimbriae via a mannose-dependent manner

“…We reviewed the published literature and found that there was strong evidence for a positive link between adherence, the presence of fimbriae, and mannosebinding activity by bacteria (Harper et al, 1978;Ofek and Beachey, 1978;Sharon, 1987Sharon, , 2006. For several (Stepinska and Trafny, 1995;King et al, 2000;McEwan et al, 2008). Earlier studies have shown that at least three different types of lectin-like proteins are responsible for the attachment of the saccharide structures of epithelial cells to the adherence proteins of gram-negative bacteria, which are mediated in a mannose-sensitive manner.…”

Comparativein vitroantimicrobial efficacy of commercial ear cleaners

“…overgrowth in canine otitis 10 . This may be due to the inclusion of monosaccharides that prevent microbial adherence and colonization 25–27 . This may lead to an artificially low estimate of antimicrobial activity in vitro , as anti‐adhesive properties would not be expected to contribute to efficacy.…”