Monoplex Nested and Semi-nested Reverse Transcription Polymerase Chain Reaction for G and P Genotyping of Human Rotavirus Group A in Clinical Specimens and Environmental Samples

El‐Senousy, Waled M.; Senna, Amel S. M. Abu; Mohsen, Nabil; Hasan, Seham F.; Kamel, Marwa A.; Sidkey, Nagwa M.

doi:10.31782/ijcrr.2020.121621

Cited by 1 publication

(3 citation statements)

References 18 publications

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“…G9 was first detected in sewage samples in 1998 [89], and subsequently detected in other studies [90,91]. G4, G10, and G12 genotypes were also detected in sewage water [89][90][91][92]. During the 2016-2017 season, RV A was detected in tap water in which G1 constituted >50% of all collected cases, followed by the G2, G4, and G9 genotypes [94].…”

Section: Distribution Of Rotavirus Strains In Environmental Samplesmentioning

confidence: 94%

“…With respect to the VP4 genotypes, P [8] was the predominant genotype detected in sewage water throughout the time period of this review [89][90][91]93]; however, P [4] was the predominant genotype in only one surveillance study [92]. P [6] was also detected in sewage water [89,91,93].…”

Section: Distribution Of Rotavirus Strains In Environmental Samplesmentioning

confidence: 98%

“…Before 2000, G1 was the predominant genotype detected in sewage water [89]. Since that time, two surveillance studies indicated that the G1 genotype was predominant during the 2015-2017 seasons [92,93]. The G2 genotype was the most predominant genotype during the 2006-2007 season [90]; however, another study G6 was the predominant genotype, followed by G10.…”

Section: Distribution Of Rotavirus Strains In Environmental Samplesmentioning

confidence: 99%

See 2 more Smart Citations

The Epidemiology of Circulating Rotavirus Associated with Diarrhea in Egyptian Kids and Calves: A Review

Ghonaim

Hopo²,

Ghonaim

et al. 2023

Zoonoses

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Acute gastroenteritis (AGE) induced by rotavirus has been a major disease burden in Egypt since 1981 when rotavirus was first reported in humans and calves. Genome segmentation facilitates the emergence of new virus serotypes, which allows reassortment during mixed infections, and is a characteristic feature of the family Reoviridae to which rotavirus belongs. The rotavirus genome involves 11 double-stranded RNA gene segments encoding 6 non-structural (NSP1–6) and 6 structural (VP1–4, VP6, VP7) proteins. Rotavirus A has a zoonotic potential associated with diarrhea. The primary strategy for prevention and control of bovine and human rotavirus infections is vaccination; however, routine rotavirus vaccination has not been implemented in the National Immunization Program. We evaluated studies published over the last 30 years that pertained to the epidemiology of circulating rotavirus genotypes among children, calves, and environmental samples in Egypt. The analysis revealed a rotavirus prevalence of 15%–100%, with diarrhea occurring throughout the year, but generally peaking during the cold months. G1 was the predominant genotype in children, followed by G2, G3, G4, G8, G9, and G12 throughout the study duration. Mixed infections were also detected. G6 was the predominant genotype in calves, followed by G10. There are still gaps in knowledge regarding molecular data of rotavirus infections in humans, animals, and environmental samples in Egypt, as well as the zoonotic potential of rotavirus disease. Therefore, it is critical to continue rotavirus surveillance in Egypt to further understand the epidemiology of rotavirus infections and the emerging new genotypes.

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Section: Distribution Of Rotavirus Strains In Environmental Samplesmentioning

confidence: 94%

Section: Distribution Of Rotavirus Strains In Environmental Samplesmentioning

confidence: 98%

Section: Distribution Of Rotavirus Strains In Environmental Samplesmentioning

confidence: 99%

See 1 more Smart Citation

The Epidemiology of Circulating Rotavirus Associated with Diarrhea in Egyptian Kids and Calves: A Review

Ghonaim

Hopo²,

Ghonaim

et al. 2023

Zoonoses

View full text Add to dashboard Cite

show abstract

Monoplex Nested and Semi-nested Reverse Transcription Polymerase Chain Reaction for G and P Genotyping of Human Rotavirus Group A in Clinical Specimens and Environmental Samples

Cited by 1 publication

References 18 publications

The Epidemiology of Circulating Rotavirus Associated with Diarrhea in Egyptian Kids and Calves: A Review

The Epidemiology of Circulating Rotavirus Associated with Diarrhea in Egyptian Kids and Calves: A Review

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