Monophosphoryl-Lipid A (MPLA) is an Efficacious Adjuvant for Inactivated Rabies Vaccines

Chen, Chen; Zhang, Chengguang; Li, Ruiming; Wang, Zongmei; Yuan, Yueming; Li, Haoqi; Fu, Zhenfang; Zhou, Ming; Zhao, Ling

doi:10.3390/v11121118

Cited by 31 publications

(17 citation statements)

References 48 publications

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“…The use of MPL in combination with Al(OH)3, in contrast to Al(OH)3 alone, induced higher titers of long-lasting binding and nAbs, while the results of memory B cells were less clear [75]. MPL might also stimulate IFNγ secretion [50,76] and enhance the recruitment of TFH [77]. Notably, Cervarix and Gardasil have shown to induce different subsets of TFH [78] and differently affect PD1 and ICOS expression on TFH [78].…”

Section: Discussionmentioning

confidence: 99%

HPV-Specific Systemic Antibody Responses and Memory B Cells are Independently Maintained up to 6 Years and in a Vaccine-Specific Manner Following Immunization with Cervarix and Gardasil in Adolescent and Young Adult Women in Vaccination Programs in Italy

et al. 2020

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Human papillomavirus (HPV) persistent infections are associated with cervical cancer and other HPV-related diseases and tumors. Thus, the characterization of long lasting immunity to currently available HPV vaccines is important. A total of 149 female subjects vaccinated with Cervarix or Gardasil participated to the study and they were stratified according to age (10–12-year-old and 16–20-year-old). Humoral immune responses (IgG and neutralizing antibody titers, antibody avidity) and circulating memory B cells were analyzed after an average of 4–6 years from the third immunization. The humoral responses against HPV-16 and HPV-18 (and HPV-6 and HPV-11 for Gardasil) were high in both age groups and vaccines up to six years from the third dose. However, Cervarix induced significantly higher and more persistent antibody responses, while the two vaccines were rather equivalent in inducing memory B cells against HPV-16 and HPV-18. Moreover, the percentage of subjects with vaccine-specific memory B cells was even superior among Gardasil vaccinees and, conversely, Cervarix vaccinated individuals with circulating antibodies, but undetectable memory B cells were found. Finally, a higher proportion of Cervarix-vaccinated subjects displayed cross-neutralizing responses against non-vaccine types HPV-31 and HPV-45. Gardasil and Cervarix may, thus, differently affect long-lasting humoral immunity from both the quantitative and qualitative point of view.

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Section: Discussionmentioning

confidence: 99%

HPV-Specific Systemic Antibody Responses and Memory B Cells are Independently Maintained up to 6 Years and in a Vaccine-Specific Manner Following Immunization with Cervarix and Gardasil in Adolescent and Young Adult Women in Vaccination Programs in Italy

et al. 2020

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“…MPLA has been shown to enhance the immunogenicity and protection of the rabies vaccine with induction of plasma cell responses. MPLA vaccinated mice accelerated the activation of dendritic cells, improving Tdependent B cell responses driving antibody production that skewed towards a strong Th1 bias (47). As seen for mild COVID-19 patients, a Th1 biased immune response is beneficial for protection protection against disease (48).…”

Section: Discussionmentioning

confidence: 99%

Inactivated rabies virus vectored SARS-CoV-2 vaccine prevents disease in a Syrian hamster model

Kurup

Malherbe

Wirblich

et al. 2021

Preprint

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SummarySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emergent coronavirus that has caused a worldwide pandemic. Although human disease is often asymptomatic, some develop severe illnesses such as pneumonia, respiratory failure, and death. There is an urgent need for a vaccine to prevent its rapid spread as asymptomatic infections accounting for up to 40% of transmission events. Here we further evaluated an inactivated rabies vectored SARS-CoV-2 S1 vaccine CORAVAX in a Syrian hamster model. CORAVAX adjuvanted with MPLA-AddaVax, a TRL4 agonist, induced high levels of neutralizing antibodies and generated a strong Th1-biased immune response. Vaccinated hamsters were protected from weight loss and viral replication in the lungs and nasal turbinates three days after challenge with SARS-CoV-2. CORAVAX also prevented lung disease, as indicated by the significant reduction in lung pathology. This study highlights CORAVAX as a safe, immunogenic, and efficacious vaccine that warrants further assessment in human trials.

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“…Although there is an abundance of computational tools designed for prophage detection in bacterial genomes 66 , experimental approaches based on stress induction remain the optimum method for discovering whether a bacterial strain harbors functional prophages, given the fact that the predictive ability of the available computational tools remains insufficient when identifying exact prophage location and uncovering signs of prophage activity 67 .…”

Section: Discussionmentioning

confidence: 99%

Putative plasmid prophages of Bacillus cereus sensu lato may hold the key to undiscovered phage diversity

Piligrimova

Kazantseva

Kazantsev

et al. 2021

Sci Rep

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Bacteriophages are bacterial viruses and the most abundant biological entities on Earth. Temperate bacteriophages can form prophages stably maintained in the host population: they either integrate into the host genome or replicate as plasmids in the host cytoplasm. As shown, tailed temperate bacteriophages may form circular plasmid prophages in many bacterial species of the taxa Firmicutes, Gammaproteobacteria and Spirochaetes. The actual number of such prophages is thought to be underestimated for two main reasons: first, in bacterial whole genome-sequencing assemblies, they are difficult to distinguish from actual plasmids; second, there is an absence of experimental studies which are vital to confirm their existence. In Firmicutes, such prophages appear to be especially numerous. In the present study, we identified 23 genomes from species of the Bacillus cereus group that were deposited in GenBank as plasmids and may belong to plasmid prophages with little or no homology to known viruses. We consider these putative prophages worth experimental assays since it will broaden our knowledge of phage diversity and suggest that more attention be paid to such molecules in all bacterial sequencing projects as this will help in identifying previously unknown phages.

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Monophosphoryl-Lipid A (MPLA) is an Efficacious Adjuvant for Inactivated Rabies Vaccines

Cited by 31 publications

References 48 publications

HPV-Specific Systemic Antibody Responses and Memory B Cells are Independently Maintained up to 6 Years and in a Vaccine-Specific Manner Following Immunization with Cervarix and Gardasil in Adolescent and Young Adult Women in Vaccination Programs in Italy

HPV-Specific Systemic Antibody Responses and Memory B Cells are Independently Maintained up to 6 Years and in a Vaccine-Specific Manner Following Immunization with Cervarix and Gardasil in Adolescent and Young Adult Women in Vaccination Programs in Italy

Inactivated rabies virus vectored SARS-CoV-2 vaccine prevents disease in a Syrian hamster model

Putative plasmid prophages of Bacillus cereus sensu lato may hold the key to undiscovered phage diversity

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