Monophasic Pulsed Microcurrent of 1–8 Hz Increases the Number of Human Dermal Fibroblasts

Yoshikawa, Yoshiyuki; Sugimoto, Masaharu; Uemura, Mikiko; Matsuo, Masafumi; Maeshige, Noriaki; Niba, Emma Tabe Eko; Shuntoh, Hisato

doi:10.2490/prm.20160005

Cited by 10 publications

(11 citation statements)

References 17 publications

(24 reference statements)

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“…α-SMA expression could be higher within 24 h after ES initiation, but the relationship between the expression of α-SMA and the duration of ES is unclear in this study. Yoshikawa et al 10 ) showed that 1-h ES with a duty cycle of 50% promotes cell proliferation. Thus, the pro-fibrotic effects of ES on HDFs might be influenced by stimulation duration.…”

Section: Discussionmentioning

confidence: 99%

“…Granulation tissue formation is crucial for wound closure, and human dermal fibroblasts (HDFs) are a key factor in this process because their migration toward wound sites, proliferation, and differentiation into myofibroblasts are necessary for granulation tissue formation 8) . Our previous studies showed that a monophasic-pulsed current (intensity; 200 μA, frequency; 2 Hz) promotes the migration of HDFs toward the cathode and their proliferation 9,10 ) , and that ES with the same conditions has a good therapeutic effect on pressure injuries 11) . Thus, we revealed the optimum intensity and frequency of ES for pressure injury healing.…”

mentioning

confidence: 99%

“…In our previous studies, we did not assess the effects of long-term stimulation in HDFs. Lowintensity or small-electric fields within 10 h showed good effects on HDFs, promoting migration and proliferation 9,10,15,16) . Zhang et al and Wang et al showed that longer ES has good effects on the migration and secretion of prohealing cytokines 14,15) ; however, the therapeutic effects and adverse effects of long-term micro current stimulation in HDFs are not clear.…”

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confidence: 99%

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Monophasic Pulsed Current Stimulation of Duty Cycle 10% Promotes Differentiation of Human Dermal Fibroblasts into Myofibroblasts

Uemura

Sugimoto²,

Yoshikawa

et al. 2021

Phys Ther Res

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Objective: Many clinical trials have shown the therapeutic effects of electrical stimulation (ES) in various conditions. Our previous studies showed that ES (200 μA and 2 Hz) promotes migration and proliferation of human dermal fibroblasts (HDFs). However, the effective duty cycle and the effect of ES on myofibroblast differentiation are unclear. This study aimed to investigate the relationship between duty cycle and myofibroblast differentiation. Methods: HDFs were subjected to ES (200 μA and 2 Hz) for 24 h with the duty cycle adapted at 0% (control), 10%, 50%, or 90%. α-smooth muscle actin (SMA) and transforming growth factor (TGF)-β1 mRNA and α-SMA protein expressions were assessed. Collagen gel contraction was observed for 48 h after ES initiation and the gel area was measured. Cell viability and pH of culture medium were analyzed for cytotoxicity of the ES. Results: Cell viabilities were decreased in the 50% and the 90% groups but ES did not influence on pH of culture media. ES with a duty cycle of 10% significantly promoted the mRNA expression of α-SMA and TGF-β1. α-SMA protein expression in the 10% group was also significantly higher than that of the control group. Collagen gel subjected to ES with a duty cycle of 10% was contracted. Conclusion: Duty cycle can influence on myofibroblast differentiation and ES with a duty cycle 10% is the effective for wound healing.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Monophasic Pulsed Current Stimulation of Duty Cycle 10% Promotes Differentiation of Human Dermal Fibroblasts into Myofibroblasts

Uemura

Sugimoto²,

Yoshikawa

et al. 2021

Phys Ther Res

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“…Even with many preliminary studies on the ES effect of monophasic waveform [31,32], monophasic signals cause irregular current flow, due to accumulated proteins on the negatively charged surface, or decrease cell viability by increasing the pH of the batch, due to oxidation [33,34]. In contrast, biphasic signals can decrease protein accumulation and oxidation on the electrode surface, because of the rapidly alternating electrode polarity within channels, through high frequency [35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Microfluidic Chip with Low Constant-Current Stimulation (LCCS) Platform: Human Nucleus Pulposus Degeneration In Vitro Model for Symptomatic Intervertebral Disc

Kim

Kwon

et al. 2021

Micromachines

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Intervertebral disc (IVD) degeneration is a major cause of low back pain (LBP) in the lumbar spine. This phenomenon is caused by several processes, including matrix degradation in IVD tissues, which is mediated by matrix metalloproteinases (MMPs) and inflammatory responses, which can be mediated by interactions among immune cells, such as macrophages and IVD cells. In particular, interleukin (IL)-1 beta (β), which is a master regulator secreted by macrophages, mediates the inflammatory response in nucleus pulposus cells (NP) and plays a significant role in the development or progression of diseases. In this study, we developed a custom electrical stimulation (ES) platform that can apply low-constant-current stimulation (LCCS) signals to microfluidic chips. Using this platform, we examined the effects of LCCS on IL-1β-mediated inflammatory NP cells, administered at various currents (5, 10, 20, 50, and 100 μA at 200 Hz). Our results showed that the inflammatory response, induced by IL-1β in human NP cells, was successfully established. Furthermore, 5, 10, 20, and 100 μA LCCS positively modulated inflamed human NP cells’ morphological phenotype and kinetic properties. LCCS could affect the treatment of degenerative diseases, revealing the applicability of the LCCS platform for basic research of electroceuticals.

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“…Moreover, according to the study by Yoshiyuki et al [ 78 ], since electrical stimulation delivered at a frequency of more than 10 Hz may suppress the proliferation of human dermal fibroblasts, the frequency was fixed 10 Hz as a minimized effective range.…”

Section: Methodsmentioning

confidence: 99%

Micro-Current Stimulation Has Potential Effects of Hair Growth-Promotion on Human Hair Follicle-Derived Papilla Cells and Animal Model

Hwang

Lee

et al. 2021

IJMS

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Recently, a variety of safe and effective non-pharmacological methods have been introduced as new treatments of alopecia. Micro-current electrical stimulation (MCS) is one of them. It is generally known to facilitate cell proliferation and differentiation and promote cell migration and ATP synthesis. This study aimed to investigate the hair growth-promoting effect of MCS on human hair follicle-derived papilla cells (HFDPC) and a telogenic mice model. We examined changes in cell proliferation, migration, and cell cycle progression with MCS-applied HFDPC. The changes of expression of the cell cycle regulatory proteins, molecules related to the PI3K/AKT/mTOR/Fox01 pathway and Wnt/β-catenin pathway were also examined by immunoblotting. Subsequently, we evaluated the various growth factors in developing hair follicles by RT-PCR in MCS-applied (MCS) mice model. From the results, the MCS-applied groups with specific levels showed effects on HFDPC proliferation and migration and promoted cell cycle progression and the expression of cell cycle-related proteins. Moreover, these levels significantly activated the Wnt/β-catenin pathway and PI3K/AKT/mTOR/Fox01 pathway. Various growth factors in developing hair follicles, including Wnts, FGFs, IGF-1, and VEGF-B except for VEGF-A, significantly increased in MCS-applied mice. Our results may confirm that MCS has hair growth-promoting effect on HFDPC as well as telogenic mice model, suggesting a potential treatment strategy for alopecia.

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Monophasic Pulsed Microcurrent of 1–8 Hz Increases the Number of Human Dermal Fibroblasts

Cited by 10 publications

References 17 publications

Monophasic Pulsed Current Stimulation of Duty Cycle 10% Promotes Differentiation of Human Dermal Fibroblasts into Myofibroblasts

Monophasic Pulsed Current Stimulation of Duty Cycle 10% Promotes Differentiation of Human Dermal Fibroblasts into Myofibroblasts

Microfluidic Chip with Low Constant-Current Stimulation (LCCS) Platform: Human Nucleus Pulposus Degeneration In Vitro Model for Symptomatic Intervertebral Disc

Micro-Current Stimulation Has Potential Effects of Hair Growth-Promotion on Human Hair Follicle-Derived Papilla Cells and Animal Model

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