Mononuclear cells from infants allergic to cow's milk secrete tumor necrosis factor α, altering intestinal function

Heyman, Martine; Darmon, Nicole; Dupont, Christophe; Dugas, Bernard; Hirribaren, A.; Blaton, Marie-Agnès; Desjeux, Jehan-François

doi:10.1016/0016-5085(94)90405-7

Cited by 190 publications

(108 citation statements)

References 31 publications

Supporting

Mentioning

102

Contrasting

Order By: Relevance

“…One hour after an OVA challenge followed by an oral dose of β-LG 30 min later, the amount of β-LG in the sera of previously sensitized rats was significantly higher compared with nonsensitized animals (Figure 2). Several models of intestinal hypersensitivity to food proteins have shown that antigen challenge of the sensitized intestine causes alterations in ion transport, permeability, and motility (Berin et al 1997;Crowe and Perdue 1992) and in the release of mediators in anaphylactic reactions such as histamine, platelet-activating factor, prostaglandins, and leukotrienes and in the release of some newly formed cytokines that have been shown to alter mucosal function in experimental models (Heyman et al 1994;Kanwar et al 1994). However, up to now it has not been fully revealed whether the increased macromolecular passage is mainly due to transcellular or paracellular transport.…”

Section: Resultsmentioning

confidence: 99%

Assessment of the allergic potential of food protein extracts and proteins on oral application using the brown Norway rat model.

Knippels¹,

Penninks²

2003

Environ Health Perspect

View full text Add to dashboard Cite

The need for widely accepted and validated animal models to test the potential allergenicity and potency of novel (biotechnology-derived) proteins has become an important issue for their safety evaluation. In this article, we summarize the results of the development of an oral sensitization protocol for food proteins in the rat. Young Brown Norway rats were exposed to either various purified allergenic proteins (e.g., ovalbumin, partly purified), a whole food (cow's milk), or total protein extracts (hen's egg white, peanut) by daily gavage dosing during 42 days without the use of an adjuvant. The results showed that Brown Norway rats can be sensitized orally to the various allergenic food proteins tested, resulting in antigen-specific immunoglobulin (Ig) G and IgE responses, without the use of adjuvants. Animals orally exposed to cow's milk or total protein extracts of egg white also developed specific IgE and IgG antibodies that recognized the same proteins compared with antibodies from patients allergic to egg white or cow's milk. We also studied local and systemic immune-mediated effects. In ovalbumin-sensitized rats, some clinical symptoms of food allergy were studied upon an oral challenge with ovalbumin. The results demonstrated that gut permeability was increased and that in some animals breathing frequency and systolic blood pressure were temporarily decreased. The results obtained show that the Brown Norway rat provides a suitable animal model for food allergy research and for the study of relative allergenicity of existing and novel food proteins.

show abstract

Section: Resultsmentioning

confidence: 99%

Assessment of the allergic potential of food protein extracts and proteins on oral application using the brown Norway rat model.

Knippels¹,

Penninks²

2003

Environ Health Perspect

View full text Add to dashboard Cite

show abstract

“…Other cell types may also be involved. In subjects allergic to cow's milk, TNF-released by mononuclear cells after milk protein challenge alters intestinal function [12]. The blocking effect of dexamethasone on aversion could be related to the inhibition of release of these mediators [13].…”

Section: Discussionmentioning

confidence: 99%

Immunological Induction of Flavour Aversion in Mice. II. Passive/Adoptive Transfer and Pharmacological Inhibition

1997

View full text Add to dashboard Cite

Mice immunized with ovalbumin develop a strong aversion to ingesting sweetened egg white dilutions or ovalbumin solutions. In immunized animals, gavage or voluntary ingestion of ovalbumin triggers an increase of vascular permeability in the intestine; pretreatment with a mixture of histamine and serotonin antagonists blocked this reaction, but not the aversion; dexamethasone inhibited both the aversion and the increase in permeability. The aversion was transferred to normal recipient mice with high-titre anti-Ova sera obtained with complete Freund's adjuvant, but not with lower-titre serum pools of mice immunized with the help of Al(OH) 3 adjuvant. However, the aversion was also (adoptively) transferred with whole spleen cells from immune donors. This later condition is inefficient to transfer the formation of high titres of specific antibodies.

show abstract

“…Tolerance induction was performed by repeated oral low-dose administration of OVA (Sigma-Aldrich). The Ag administration schedule was 1 mg of whole OVA by gastric intubation for 5 consecutive days (days [1][2][3][4][5]. To compare the effect of systemic vs hepatic IL-10 on oral tolerance, we separately treated non-TG animals with i.p.…”

Section: Tolerance Protocolmentioning

confidence: 99%

“…Exposure to harmless Ags through the oral route eventually results in immunologic hyporesponsiveness or oral tolerance (1)(2)(3)(4)(5). The primary mechanisms underlying oral tolerance are thought to include T cell deletion, anergy, and suppression (6,7).…”

mentioning

confidence: 99%

Enhanced Oral Tolerance in Transgenic Mice with Hepatocyte Secretion of IL-10

Safadi

Álvarez

Ohta

et al. 2005

The Journal of Immunology

View full text Add to dashboard Cite

Several cytokines derived from Th3 and Tr1 cells, including IL-10, are believed to regulate oral tolerance, but direct evidence is lacking. We have explored the potential role of IL-10 by generating transgenic (TG) mice with sustained hepatocyte-specific expression of rat IL-10. TG mice expressed rat IL-10 downstream of a transthyretin promoter, which led to serum levels that were increased 10- to 100-fold compared with normal animals. Animals were orally administered 1 mg of whole OVA for 5 consecutive days, with control animals receiving PBS. There were six animal groups: Either OVA or PBS were fed orally to rat IL-10 TG mice, non-TG wild-type mice without IL-10 administration, and non-TG wild-type mice administered rat IL-10 systemically. On day 8, all mice were immunized with two injections of OVA, and then analyzed on day 18. T cell proliferation responses were reduced by 65.8 ± 14.3% after feeding of OVA in rIL-10 TG animals, compared with 39.4 ± 15.6% in the non-TG mice (p = 0.02). Anti-OVA titers were expressed as fold increase over naive non-TG mice. After feeding, titers decreased by ∼33% (from 3- to 2-fold) in TG animals and, to a lesser extent, in non-TG animals. IFN-γ secretion by cultured popliteal lymphocytes decreased in TG animals by 83% after feeding and by 69% in non-TG animals. IL-4 secretion increased 4-fold in TG-fed mice, but did not significantly change in non-TG OVA-fed animals. In contrast to hepatic TG expression of rIL-10, systemic administration of rIL-10 had only a modest effect on tolerance. IL-10, when transgenically expressed in the liver enhances mucosal tolerance to an oral Ag.

show abstract

Mononuclear cells from infants allergic to cow's milk secrete tumor necrosis factor α, altering intestinal function

Cited by 190 publications

References 31 publications

Assessment of the allergic potential of food protein extracts and proteins on oral application using the brown Norway rat model.

Assessment of the allergic potential of food protein extracts and proteins on oral application using the brown Norway rat model.

Immunological Induction of Flavour Aversion in Mice. II. Passive/Adoptive Transfer and Pharmacological Inhibition

Enhanced Oral Tolerance in Transgenic Mice with Hepatocyte Secretion of IL-10

Contact Info

Product

Resources

About