Mononuclear cells from HIV-infected patients produce factors which enhance functional activity of polymorphonuclear neutrophils from healthy subjects

Gabrilovich, Dmitry I.; Shepeleva, G. K.; Serebrovskaya, L. V.; Avdeeva, L. A.; Suvorova, Z. K.; Rosly, I. M.; Oganezov, V. K.; Saidov, M. Z.; Panyutich, Alexander V.; Pokrovsky, Vadim

doi:10.1111/j.1365-2249.1992.tb06963.x

Cited by 6 publications

(1 citation statement)

References 24 publications

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“…Gabrilovichc/a/. [18] recently reported the production of a soluble factor which enhances chemiluminescencc and chemotaxis of neutrophils by mononuclear cells from HIV-infected patients. If eireulating IL-8 acts as a ehemoattraetant in riro.…”

Section: Statistical Anaiysismentioning

confidence: 99%

Elevated serum levels of IL-8 in patients with HIV infection

Matsumoto

Miike

Nelson

et al. 1993

Clinical and Experimental Immunology

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SUMMARYScrum levels oflI.-8 were determined in HlV-infected individuals and the results were compared with those for HIV controls. The IL-8 levels were measured by an ELISA wilh a MoAband a polyelonal antibody to recombinant IL-8. The means and 95"!, confidence intervals of lL-8 in sem of 36 HIVinfected individuals and 32 matched controls were 275 and 216 .349 pg/ml, and 8 and 4 14 pg/ml. respeelively. showing a 34-fold increase in IL-8 in the eirculation of HlV-infected individuals. This increase does not appear to be related to the disease stale, infection or systemic medical agents. This finding suggests the possible involvement of IL-8 in the pathogenesis of HIV-induced disease.

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Section: Statistical Anaiysismentioning

confidence: 99%

Elevated serum levels of IL-8 in patients with HIV infection

Matsumoto

Miike

Nelson

et al. 1993

Clinical and Experimental Immunology

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Priming study of human phagocytes oxidative burst by using flow cytometry

Elbim

Gougerot‐Pocidalo

1996

Hematol Cell Ther

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In response to a variety of stimuli, e.g. pathogens, phagocytes release reactive oxygen species which are essential for bacterial killing and also potentiate inflammatory reactions. We have used flow cytometry measurements to study the priming process of phagocyte oxidative burst in whole blood, in order to avoid introducing artefacts due to the purification process and to stimulate the in vivo situation more closely. In these conditions, we examined the in vitro effects of proinflammatory cytokines (TNF-alpha, IL-1 alpha, IL-1 beta, IL-6, IL-8 and GM-CSF) on the PMN oxidative burst. We found that none of the cytokine tested directly activated the PMN oxidative burst. In contrast, TNF, GM-CSF and IL-8 strongly primed a subpopulation of PMN which produced large amounts of H2O2 in response to fMLP, suggesting that these cytokines may play a critical role in bactericidal killing in vivo. Furthermore, we reported a decreased H2O2 production by TNF or IL-8 primed PMN in HIV-infected patients. This impairment, which correlated with the clinical stage of the disease, could contribute to the increased susceptibility to bacterial infections in HIV-infected patients. In addition, we reported the case of a child with severe recurrent infections due to intracellular microorganisms which could be related to an impairment of the phagocyte priming process of the oxidative burst [corrected].

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