Mononuclear cell subpopulations in the skin defined by monoclonal antibodies after HLA-identical sibling marrow transplantation

Abstract

Mononuclear cell subpopulations present in the skin of 36 recipients of HLA-identical sibling marrow transplants were defined by immunoperoxidase using a battery of monoclonal antibodies to cell surface differentiation antigens. The T4-positive (T4+) (helper-inducer T cells), T8+ (cytotoxic-suppressor T cells) and the T6+ (Langerhans cells) decreased in number early post transplant and returned towards normal numbers from day 42 onwards. There was no evidence that either the T4+ or the T8+ subset was involved in cell-to-cell contact damage in acute graft-versus-host disease (GVHD). The paucity of lymphoid cell infiltration of the epidermis in acute GVHD suggested the possibility of a soluble factor being responsible for basal layer damage. In patients with chronic GVHD there was no evidence of T4+ lymphocyte involvement, but T8+ lymphocytes were present in increased numbers, suggesting a role for the T8+ population in the skin lesions of chronic GVHD, or possibly a reflection of the pattern of T4+ and T8+ cell reconstitution in the blood post-transplant. Finally, our study provided no evidence that BI+ (B cells), Leu 7+ (natural killer cells), OKMI+ (histiocytes) or OKT1O+ cells were involved in cell-to-cell contact damage in either acute or chronic GVHD.

Characterization of cellular infiltrate, detection of chemokine receptor CCR5 and interleukin‐8 and RANTES chemokines in adult periodontitis

“…The topographical distribution of positively immunostained cells in the in®ltrating connective tissue (ICT) was established, using consecutive tissue sections as previously described (31,32). A range between 0 and 4 was used to establish the proportion of total in¯ammatory cells present in the in¯ammatory in®ltrate: 0~no detectable cells; 1z~less than 10%; 2z~approx.…”

Interferon‐γ, Interleukins‐6 and ‐4, and Factor XIII‐A as Indirect Markers of the Classical and Alternative Macrophage Activation Pathways in Chronic Periodontitis

“…Labeled cells were quantified using the methodology described and validated by Atkinson et al 18 and Gamonal et al 19 Quantification was performed for markers of IFN‐γ, IL‐6, IL‐4, and FXIII‐A gingival tissue samples per field counting the total number of inflammatory cells positive for immunohistochemical staining, total count of stained cells for markers of CD68, IFN‐γ, IL‐6, IL‐4, and FXIII‐A, and as percentages of the total number of inflammatory cells (Table 1).…”

“…****** The images were analyzed using software. † † † † † † Quantification Labeled cells were quantified using the methodology described and validated by Atkinson et al 18 and Gamonal et al 19 inflammatory cells positive for immunohistochemical staining, total count of stained cells for markers of CD68, IFN-g, IL-6, IL-4, and FXIII-A, and as percentages of the total number of inflammatory cells (Table 1).…”

Acute graft-versus-host disease following hematopoietic stem-cell transplantation

“…While many studies have been conducted to determine the nature of the lymphocyte subpopulations found in the skin of patients with acute GVHD, unfortunately, the results are inconclusive at best (72–84). The infiltrate is composed of T cells, but both CD4+ and CD8+ subsets have shown to predominate (72–84).…”

“…While many studies have been conducted to determine the nature of the lymphocyte subpopulations found in the skin of patients with acute GVHD, unfortunately, the results are inconclusive at best (72)(73)(74)(75)(76)(77)(78)(79)(80)(81)(82)(83)(84). The infiltrate is composed of T cells, but both CD4+ and CD8+ subsets have shown to predominate (72)(73)(74)(75)(76)(77)(78)(79)(80)(81)(82)(83)(84). In recent years, plasma and urine proteomics have shown promise in enabling early diagnosis of acute GVHD (85)(86)(87)(88).…”