Mononuclear cell subpopulations in CSF and blood of children with bacterial meningitis

Ichiyama, Takashi; Kajimoto, Madoka; Matsushige, Takeshi; Shiraishi, Masaya; Suzuki, Yasuo; Furukawa, Susumu

doi:10.1016/j.jinf.2008.10.012

Cited by 4 publications

(9 citation statements)

References 21 publications

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“…[17]. Однако, в отличие от данных этих авторов, у обследованных нами детей с БГМ не отмече-но достоверного повышения доли CD3 + , CD4 + , CD8 + Т-клеток в ЦСЖ по сравнению с кровью.…”

Section: Discussionunclassified

“…Однако, в отличие от данных этих авторов, у обследованных нами детей с БГМ не отмече-но достоверного повышения доли CD3 + , CD4 + , CD8 + Т-клеток в ЦСЖ по сравнению с кровью. Несовпадения с данными [17] могут быть обус-ловлены различиями этиологии БГМ. В ис-следовании Ishiyama T. с соавт.…”

Section: Discussionunclassified

“…Оценить отклонения в распределении суб-популяций лимфоцитов в ЦСЖ пациентов от показателей в нормальном ЦСЖ у здоровых детей оказалось невозможным, так как спин-номозговая пункция проводится строго по по-казаниям, и в доступных публикациях группы контроля отсутствуют [17,20,21,24,29]. Боль-шой интерес, однако, представляет сопоставле-ние этих показателей в крови и ЦСЖ детей с ВМ (см.…”

Section: результатыunclassified

“…До сих пор основным лабораторным критерием ранней дифференциальной диагностики между серозными и гнойными менингитами служат различия пула лейкоцитов крови в церебро-спинальной жидкости (ЦСЖ) -умеренный лимфоцитарный плеоцитоз при серозном и вы-сокий нейтрофильный плеоцитоз при гнойном менингите [13]. Многие исследователи разделя-ют мнение о необходимости расширения имму-нологических критериев диагностики заболева-ний центральной нервной системы (ЦНС) путем оценки соотношения различных субпопуляций лимфоцитов непосредственно в интратекаль-ном пространстве [15,17,19,20,22].…”

Section: Introductionunclassified

“…В большинстве работ иммунофенотипирование клеток проводили параллельно в крови и ЦСЖ, используя этот подход для прояснения особен-ностей иммунопатогенеза различных заболе-ваний ЦНС. Следует отметить, что за послед-ние 20 лет фенотипирование клеток ЦСЖ при менингитах у детей проводилось сравнительно редко [17,20,21,24,29]. Обращает на себя вни-мание также недостаток исследований клеток с маркером ранней активации -CD25(IL-2Rα), готовых к пролиферации и дифференцировке, и маркером поздней активации -CD95(Fas/ Apo-1), готовых к апоптозу через взаимодей-ствие Fas/Fas-лиганд [12].…”

Section: Introductionunclassified

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Lymphocyte Subsets and Cytokines in Blood and Cerebrospinal Fluid in Children With Viral and Bacterial Meningitis

Алексеева

Федоровна

Жирков

et al. 2016

Russian Journal of Infection and Immunity

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Introduction of flow cytometry caused an increase in the investigation of liquor lymphocyte pool phenotype in the case of different brain disorders, including viral and bacterial meningitis, however this type of research in children has been relatively rare. Phenotype and lymphocyte functions are under cytokine control system, therefore detection of interconnections between lymphocyte pool subpopulation composition and cytokine level in blood and liquor of the patients concerns a great interest. The purpose of this research was to study lymphocyte subpopulation composition and the level of cytokines IL-1β, IL-6, IL-8, IL-10, IFNα, IFNγ and IL-4, and also IgG in liquor and blood of children with viral and bacterial meningitis. There was performed blood and liquor investigation in 46 children aged from 1 to 16 years old with viral (n = 35) and bacterial (n = 11) meningitis. Immunophenotyping of blood and liquor cells was performed by the method of flow cytometry with the use of monoclonal antibodies to CD3, CD4, CD8, CD19, CD16, CD56, CD25 and CD95. The content of cytokines was detected in ELISA, and that of IgG — by the method of quantitative immunoturbodimetry. During an acute period of viral meningitis there was detected a decrease in NK portion and activated CD25+ cells in the blood of patients accompanied by the increase in B-lymphocytes number, along with cytokine IFNγ, IL-8 and IL-10 serum level rise. There was determined T-lymphocytes accumulation in liquor with the prevalence of CD4+ Т-cells and, to a lesser degree, CD25+ and CD95+ cells, NK and B-lymphocytes. Intrathecally there was noted the predominance of IL-6 response accompanied by the growth of IL-8 and IL-10 concentration as well. During an acute period of bacterial meningitis there was noted a decrease in percentage of CD3+, CD4+, CD8+ Т-lymphocytes, NK, CD25+ and CD95+ cells, along with, on the contrary, sharp increase in B-cells pool, simultaneously with an expressed system response of IL-8 and IL-10. Liquor T-lymphocyte content was relatively correlated with blood indicators whereas the fraction of NK exceeded it, and B-lymphocyte content was 3–4 times higher than in patients with viral meningitis. There was IL-6, IL-10, IFNγ and IL-4 response intrathecally, and 10-multiply-growth of IgG level. Thus, the redistribution of lymphocyte subpopulations, and system and local cytokine response in children with meningitis have both common and special features depending on the aetiology and severity of disease. Phenotyping of lymphocytes and determination of both cytokines and immunoglobulins simultaneously in two biologic fluid allow to clear up the pathogenetic value of immunologic abnormalities in blood and cerebrospinal fluid of the patients in the aspect of interactions between cell and humoral factors of system and local immune response in neuroinfections of various aetiology.

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Section: Discussionunclassified

Section: результатыunclassified

Section: Introductionunclassified

See 3 more Smart Citations

Lymphocyte Subsets and Cytokines in Blood and Cerebrospinal Fluid in Children With Viral and Bacterial Meningitis

Алексеева

Федоровна

Жирков

et al. 2016

Russian Journal of Infection and Immunity

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1 H NMR spectral identification of medication in cerebrospinal fluid of pediatric meningitis

Mason

Solomons

Wevers

et al. 2017

Journal of Pharmaceutical and Biomedical Analysis

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Pathogenic Triad in Bacterial Meningitis: Pathogen Invasion, NF-κB Activation, and Leukocyte Transmigration that Occur at the Blood-Brain Barrier

et al. 2016

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Bacterial meningitis remains the leading cause of disabilities worldwide. This life-threatening disease has a high mortality rate despite the availability of antibiotics and improved critical care. The interactions between bacterial surface components and host defense systems that initiate bacterial meningitis have been studied in molecular and cellular detail over the past several decades. Bacterial meningitis commonly exhibits triad hallmark features (THFs): pathogen penetration, nuclear factor-kappaB (NF-κB) activation in coordination with type 1 interferon (IFN) signaling and leukocyte transmigration that occur at the blood-brain barrier (BBB), which consists mainly of brain microvascular endothelial cells (BMEC). This review outlines the progression of these early inter-correlated events contributing to the central nervous system (CNS) inflammation and injury during the pathogenesis of bacterial meningitis. A better understanding of these issues is not only imperative to elucidating the pathogenic mechanism of bacterial meningitis, but may also provide the in-depth insight into the development of novel therapeutic interventions against this disease.

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Mononuclear cell subpopulations in CSF and blood of children with bacterial meningitis

Cited by 4 publications

References 21 publications

Lymphocyte Subsets and Cytokines in Blood and Cerebrospinal Fluid in Children With Viral and Bacterial Meningitis

Lymphocyte Subsets and Cytokines in Blood and Cerebrospinal Fluid in Children With Viral and Bacterial Meningitis

1 H NMR spectral identification of medication in cerebrospinal fluid of pediatric meningitis

Pathogenic Triad in Bacterial Meningitis: Pathogen Invasion, NF-κB Activation, and Leukocyte Transmigration that Occur at the Blood-Brain Barrier

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