Mononitrosyl-iron complexes with dithiocarbamate ligands: physico-chemical properties

Vanin, Anatoly F.; Faassen, Ernst van

doi:10.1016/b978-044452236-8/50018-6

Cited by 6 publications

(4 citation statements)

References 42 publications

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“…Cd 2+ -induced NO production was confirmed by electron paramagnetic resonance (EPR) spectroscopy. The detection was performed with the NO-specific spin trap Fe(II) plus N-methyl-D-glucamine complex [(MGD) 2 Fe(II); Vanin and van Faassen, 2007]. Cells treated for 48 h with 150 mM CdCl 2 clearly presented the typical hyperfine structure triplet of the NO complex, whereas untreated cells exhibited only a faint signal, indicating that Cd 2+ exposure led to a strong NO production (Fig.…”

Section: +mentioning

confidence: 99%

Nitric Oxide Is Involved in Cadmium-Induced Programmed Cell Death in Arabidopsis Suspension Cultures

Michele

Vurro²,

Rigo³

et al. 2009

Plant Physiology

248

161

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Exposure to cadmium (Cd 2+ ) can result in cell death, but the molecular mechanisms of Cd 2+ cytotoxicity in plants are not fully understood. Here, we show that Arabidopsis (Arabidopsis thaliana) cell suspension cultures underwent a process of programmed cell death when exposed to 100 and 150 mM CdCl 2 and that this process resembled an accelerated senescence, as suggested by the expression of the marker senescence-associated gene12 (SAG12). CdCl 2 treatment was accompanied by a rapid increase in nitric oxide (NO) and phytochelatin synthesis, which continued to be high as long as cells remained viable. Hydrogen peroxide production was a later event and preceded the rise of cell death by about 24 h. Inhibition of NO synthesis by N G -monomethylarginine monoacetate resulted in partial prevention of hydrogen peroxide increase, SAG12 expression, and mortality, indicating that NO is actually required for Cd 2+ -induced cell death. NO also modulated the extent of phytochelatin content, and possibly their function, by S-nitrosylation. These results shed light on the signaling events controlling Cd 2+ cytotoxicity in plants.

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Section: +mentioning

confidence: 99%

Nitric Oxide Is Involved in Cadmium-Induced Programmed Cell Death in Arabidopsis Suspension Cultures

Michele

Vurro²,

Rigo³

et al. 2009

Plant Physiology

248

161

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“…Intensity of Fe(DETC) 2 NO signals in tumors was estimated as the amplitude of the third (high-field [28,[70][71][72]86,87]) constituent of the hyperfine structure. Particular examples of quantification of the spectra [25,34] are depicted in Figure 7. Simulations of EPR spectra of various types of HbNO complexes are presented in the cited paper: Dutka et al, 2019 (a chapter) [25].…”

Section: Epr Measurementmentioning

confidence: 99%

“…Consequently, in EPR-measurements in vivo, exogenous spin traps are usually used, mainly diethyldithiocarbamate (DETC) [31] and its derivatives [32]. Two molecules of the trap initially chelate Fe 2+ ion (endogenous or exogenous), which eventually coordinates NO to yield the characteristic, EPR-detectable nitrosyl complex Fe(DETC) 2 NO [33], also called the mononitrosyl-iron complex (MNIC) [34]. Even in this case, however, the yield of NO complexing is not the same throughout the tumor.…”

Section: Introductionmentioning

confidence: 99%

Spin Trapping of Nitric Oxide by Hemoglobin and Ferrous Diethyldithiocarbamate in Model Tumors Differing in Vascularization

Szczygieł,

Łaś

et al. 2024

IJMS

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Animal tumors serve as reasonable models for human cancers. Both human and animal tumors often reveal triplet EPR signals of nitrosylhemoglobin (HbNO) as an effect of nitric oxide formation in tumor tissue, where NO is complexed by Hb. In search of factors determining the appearance of nitrosylhemoglobin (HbNO) in solid tumors, we compared the intensities of electron paramagnetic resonance (EPR) signals of various iron–nitrosyl complexes detectable in tumor tissues, in the presence and absence of excess exogenous iron(II) and diethyldithiocarbamate (DETC). Three types of murine tumors, namely, L5178Y lymphoma, amelanotic Cloudman S91 melanoma, and Ehrlich carcinoma (EC) growing in DBA/2 or Swiss mice, were used. The results were analyzed in the context of vascularization determined histochemically using antibodies to CD31. Strong HbNO EPR signals were found in melanoma, i.e., in the tumor with a vast amount of a hemorrhagic necrosis core. Strong Fe(DETC)2NO signals could be induced in poorly vascularized EC. In L5178Y, there was a correlation between both types of signals, and in addition, Fe(RS)2(NO)2 signals of non-heme iron–nitrosyl complexes could be detected. We postulate that HbNO EPR signals appear during active destruction of well-vascularized tumor tissue due to hemorrhagic necrosis. The presence of iron–nitrosyl complexes in tumor tissue is biologically meaningful and defines the evolution of complicated tumor–host interactions.

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“…O estado fundamental desta molécula possui multiplicidade 2 (dubleto), em acordo com o que foi discutido em [232]. A geometria otimizada é exibida na Figura 23 enquanto os respectivos parâmetros geométricos, em Å, estão presentes no Anexo A.…”

Section: R/aunclassified

Estrutura eletrônica, espectroscopia e dinâmica quase-clássica de sistemas moleculares: um estudo teórico

Silva¹

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Neste trabalho, cálculos de estrutura eletrônica foram realizados visando investigar espécies formadas de [Li,N,O,S]. Através deste estudo, foi possível identificar que o acoplamento spin-órbita possui papel fundamental nos espectros moleculares dos diátomos N2, NO e LiO. Em particular, usamos os resultados obtidos para o radical NO a fim de examinar a técnica de aprisionamento por spin. Esta técnica tem por finalidade detectar radicais livres com de curtos tempos de vida em sistemas biológicos. Uma proposta de construção de superfície de energia potencial para a espécie LiS2 é discutida utilizando como base os resultados para a diatômica LiS. Para o N2, nossas predições indicam um novo sistema de bandas nunca antes observado. Além do mais, considerando a metodologia das trajetórias quase-clássicas, analisou-se a formação do mínimo de Van der Waals ArSO2 gerado a partir de colisões entre o átomo de argônio e o dióxido de enxofre.

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Mononitrosyl-iron complexes with dithiocarbamate ligands: physico-chemical properties

Cited by 6 publications

References 42 publications

Nitric Oxide Is Involved in Cadmium-Induced Programmed Cell Death in Arabidopsis Suspension Cultures

Nitric Oxide Is Involved in Cadmium-Induced Programmed Cell Death in Arabidopsis Suspension Cultures

Spin Trapping of Nitric Oxide by Hemoglobin and Ferrous Diethyldithiocarbamate in Model Tumors Differing in Vascularization

Estrutura eletrônica, espectroscopia e dinâmica quase-clássica de sistemas moleculares: um estudo teórico

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