Monolith columns for liquid chromatographic separations of intact proteins: A review of recent advances and applications

Lynch, Kyle B.; Ren, Jiangtao; Beckner, Matthew A.; He, Chiyang; Liu, Shaorong

doi:10.1016/j.aca.2018.09.021

Cited by 73 publications

(46 citation statements)

References 240 publications

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“…Among them are the improved protein separation due to efficient mass transport and the use of higher flow rates. This shortens the time of analysis, allowing implementation of kinetic studies [30][31][32]. Monolithic columns are also more robust and enable analyses of much larger series of serum samples than particle packed columns [33].…”

Section: Introductionmentioning

confidence: 99%

Binding Kinetics of Ruthenium Pyrithione Chemotherapeutic Candidates to Human Serum Proteins Studied by HPLC-ICP-MS

et al. 2020

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The development of ruthenium-based complexes for cancer treatment requires a variety of pharmacological studies, one of them being a drug’s binding kinetics to serum proteins. In this work, speciation analysis was used to study kinetics of ruthenium-based drug candidates with human serum proteins. Two ruthenium (Ru) complexes, namely [(η6-p-cymene)Ru(1-hydroxypyridine-2(1H)-thionato)Cl] (1) and [(η6-p-cymene)Ru(1-hydroxypyridine-2(1H)-thionato)pta]PF6 (2) (where pta = 1,3,5-triaza-7-phosphaadamantane), were selected. Before a kinetics study, their stability in relevant media was confirmed by nuclear magnetic resonance (NMR). Conjoint liquid chromatography (CLC) monolithic column, assembling convective interaction media (CIM) protein G and diethylamino (DEAE) disks, was used for separation of unbound Ru species from those bound to human serum transferrin (Tf), albumin (HSA) and immunoglobulins G (IgG). Eluted proteins were monitored by UV spectrometry (278 nm), while Ru species were quantified by post-column isotope dilution inductively coupled plasma mass spectrometry (ID-ICP-MS). Binding kinetics of chlorido (1) and pta complex (2) to serum proteins was followed from 5 min up to 48 h after incubation with human serum. Both Ru complexes interacted mainly with HSA. Complex (1) exhibited faster and more extensive interaction with HSA than complex (2). The equilibrium concentration for complex (1) was obtained 6 h after incubation, when about 70% of compound was bound to HSA, 5% was associated with IgG, whereas 25% remained unbound. In contrast, the rate of interaction of complex (2) with HSA was much slower and less extensive and the equilibrium concentration was obtained 24 h after incubation, when about 50% of complex (2) was bound to HSA and 50% remained unbound.

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Section: Introductionmentioning

confidence: 99%

Binding Kinetics of Ruthenium Pyrithione Chemotherapeutic Candidates to Human Serum Proteins Studied by HPLC-ICP-MS

et al. 2020

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“…Monolith columns have strong merits in fast analysis of large molecules such as proteins and polynucleotides since separation efficiency is not degraded significantly at a high flow rate owing to good mass transfer kinetics based on high permeability . However, the operational conditions of analyte separation are quite different from those of packed columns, and the absolute amount of stationary phase is quite smaller than that of packed column owing to excessive volume of macropores so that application of monolithic columns to conventional HPLC characterization is rather limited .…”

Section: The Average and Standard Deviation Data Of Column Separationmentioning

confidence: 99%

Development of Ground Organic Monolith Particles as Packing Material in High Performance Liquid Chromatography

Kim

Cheong

2020

Bulletin Korean Chem Soc

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“…As a good alternative to particle‐packed columns, monolithic columns exhibiting higher permeability and faster mass transfer are applied in either nano‐LC or cLC to increase the sensitivity in proteome analysis . Recently, there are several review articles focusing on the development of monolithic columns for chromatographic analysis of biomolecules . Herein, the application of hybrid monolithic materials in microscale bioseparation of proteins and peptides would be mainly presented.…”

Section: Monolithic Materials For High Efficient Chromatographic Sepamentioning

confidence: 99%

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Wang

et al. 2019

Advanced Materials

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High‐performance liquid chromatography integrated with tandem mass spectrometry (HPLC–MS/MS) has become a powerful technique for proteomics research. Its performance heavily depends on the separation efficiency of HPLC, which in turn depends on the chromatographic material. As the “heart” of the HPLC system, the chromatographic material is required to achieve excellent column efficiency and fast analysis. Monolithic materials, fabricated as continuous supports with interconnected skeletal structure and flow‐through pores, are regarded as an alternative to particle‐packed columns. Such materials are featured with easy preparation, fast mass transfer, high porosity, low back pressure, and miniaturization, and are next‐generation separation materials for high‐throughput proteins and peptides analysis. Herein, the recent progress regarding the fabrication of various monolithic materials is reviewed. Special emphasis is placed on studies of the fabrication of monolithic capillary columns and their applications in separation of biomolecules by capillary liquid chromatography (cLC). The applications of monolithic materials in the digestion, enrichment, and separation of phosphopeptides and glycopeptides from biological samples are also considered. Finally, advances in comprehensive 2D HPLC separations using monolithic columns are also shown.

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Monolith columns for liquid chromatographic separations of intact proteins: A review of recent advances and applications

Cited by 73 publications

References 240 publications

Binding Kinetics of Ruthenium Pyrithione Chemotherapeutic Candidates to Human Serum Proteins Studied by HPLC-ICP-MS

Binding Kinetics of Ruthenium Pyrithione Chemotherapeutic Candidates to Human Serum Proteins Studied by HPLC-ICP-MS

Development of Ground Organic Monolith Particles as Packing Material in High Performance Liquid Chromatography

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

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