Monolith and coating enzymatic microreactors of l-asparaginase: kinetics study by MCE–LIF for potential application in acute lymphoblastic leukemia (ALL) treatment

Qiao, Juan; Qi, Li; Mu, Xiaoyu; Chen, Yi

doi:10.1039/c1an15067g

Cited by 25 publications

(23 citation statements)

References 30 publications

(34 reference statements)

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“…Low hemolytic WRK variant, WarnG20D, according to its anti-leukemia activity could be good candidates for treatment of leukemia. However, because of the putative cytotoxicity of the peptide for cells other than MNC, it would be interesting to test the efficacy for leukemia therapy of WarnG20D immobilized in an extracorporeal shunt system as proposed by Qiao et al [52] for the anticancer enzyme L-Asnase.…”

Section: Resultsmentioning

confidence: 99%

Specific Anti-Leukemic Activity of the Peptide Warnericin RK and Analogues and Visualization of Their Effect on Cancer Cells by Chemical Raman Imaging

Loiseau¹,

Augenstreich²,

Marchand³

et al. 2016

PLoS ONE

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Antimicrobial peptides can be used as therapeutic agents against cancer cells. Warnericin RK and derivatives (WarnG20D and WarnF14V) were tested on various, solid tumor or leukemia, cancer cells. These peptides appeared to be cytotoxic on all the cell types tested, cancerous as well healthy, but very interestingly displayed no deleterious effect on healthy mononuclear cells. The mode of action of the peptide was proposed to be membranolytic, using chemical Raman imaging. Addition of peptide induced a large disorganization of the membrane leading to the loss of the content of inner compartments of Jurkat cell, whereas no effect was observed on the healthy mononuclear cells. The less hemolytic peptides WarnG20D and WarnF14V could be good candidates for the leukemia treatment.

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Section: Resultsmentioning

confidence: 99%

Specific Anti-Leukemic Activity of the Peptide Warnericin RK and Analogues and Visualization of Their Effect on Cancer Cells by Chemical Raman Imaging

Loiseau¹,

Augenstreich²,

Marchand³

et al. 2016

PLoS ONE

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“…Thus, Qiao et al have reported a series of papers on assays for l-asparaginase [161][162][163] which catalyzed the deamidation of Asn and Gln to Asp and Glu, respectively. After enzyme incubation the analytes were derivatized with fluorescein isothiocyanate for microchip electrophoretic separation using LIF-detection.…”

Section: Enzyme Assays Using Microfluidic Devicesmentioning

confidence: 97%

“…After enzyme incubation the analytes were derivatized with fluorescein isothiocyanate for microchip electrophoretic separation using LIF-detection. For offline IMERs the enzyme was immobilized to a glycidyl methacrylate-ethylene dimethacrylate-based monolith [162] or adsorbed to gold nanoparticles that were subsequently immobilized to a (3-mercaptopropyl)-trimethoxysilane-treated capillary [163]. The assays were applied to the determination of the enzyme kinetic data [161][162][163] as well as the deamidation of Asn spiked to human plasma [162].…”

Section: Enzyme Assays Using Microfluidic Devicesmentioning

confidence: 99%

Advances in Capillary Electrophoresis-Based Enzyme Assays

Scriba

Belal

2015

Chromatographia

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IntroductionEnzymes catalyze metabolic reactions in cells regulating homeostasis, growth and reproduction of living organisms. Enzymes are also often involved in human disease. The analysis of the human genome has revealed that within the so-called druggable genome a significant portion (in some analyses more than half) of the potential drug targets are enzymes [1][2][3]. Consequently, enzymes are important targets in the development of new drugs. Moreover, enzymes play a role in the clinical diagnosis of diseases. Therefore, effective methods for characterization of enzymes as well as for the determination of their activity are important, for example, for the understanding of enzyme-mediated metabolic reactions or the identification of substrates and inhibitors for the treatment of human diseases.Capillary electrophoresis (CE) has been applied to enzyme analysis for more than 20 years since the first report by Banke et al. on an assay of alkaline protease [4]. Since then, all aspects of enzyme-related analysis have been studied by CE methods including the evaluation of enzymatic activity, enzyme kinetics, identification and characterization of enzyme inhibitors and activators as well as metabolic pathways as, for example, summarized in [5][6][7][8][9][10][11][12].Generally, CE-based enzyme assays can be divided in three groups, pre-capillary (offline) assays, in-capillary (online) assays and post-capillary assays. In the pre-capillary assays, all required components including enzyme, substrate, co-factors, etc., are mixed in a vial and incubated for an intended period of time. Subsequently, the reaction is quenched and an aliquot is subjected to CE analysis for the determination of substrate(s) and/or co-substrate(s) and/ or product(s). Multiple sampling or repeated sampling in combination with sequential runs for the determination of Abstract Capillary electrophoresis (CE) has become a flexible and accurate, high-efficiency analytical separation technique in many areas requiring only minute amounts of sample and chemicals. Thus, CE has also been recognized as a suitable technique to study enzymatic reactions including the determination of Michaelis-Menten kinetic data or the identification and characterization of inhibitors. The most often applied CE-based enzyme assay modes can be divided into two categories: (1) pre-capillary assays where incubations are performed offline followed by CE analysis of substrate(s) and/or product(s) and (2) in-capillary assays in which the enzymatic reaction and analyte separation are performed in the same capillary. In case of the in-capillary assays, the enzyme may be immobilized or in solution. The latter is also referred to as electrophoretically mediated microanalysis (EMMA), while in the case of immobilized enzyme the term immobilized enzyme reactor (IMER) is used. The present review summarizes the literature on CEbased enzyme assays published between January 2010 and April 2015. Immobilized enzyme reactors as well as microfluidic devices applied to the study of enzymatic a...

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“…It is evident that the drawback of lower concentration LODs is being overcome. Therefore, a growing number of on-chip enzyme assays overtaking macroscale methods even in terms of the concentration LODs can be expected [100].…”

Section: Enzyme Assays In Microchip Formatmentioning

confidence: 99%

Microscale separation methods for enzyme kinetics assays

Křížek

Kubíčková

2012

Anal Bioanal Chem

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Miniaturization continues to be one of the leading trends in analytical chemistry and one that brings advantages that can be particularly beneficial in biochemical research. Use of a miniaturized scale enables efficient analysis in a short time and requires very small amounts of samples, solvents, and reagents. This can result in a remarkable decrease in costs of enzyme kinetics studies, especially when expensive or rare enzymes and/or substrates are involved. Free zone electrophoresis is without a doubt the most common microscale separation technique for capillary and on-chip enzyme assays. Progress and applications in this field are reviewed frequently whereas other modes of separation, although successfully applied, receive only marginal interest in such publications. This review summarizes applications of less common modes of separation in capillary or chip formats, namely micellar electrokinetic chromatography, liquid chromatography, gel electrophoresis, isoelectric focusing, and isotachophoresis. Because these techniques are based on separation mechanisms different from those of free zone electrophoresis, they can be, and have been, successfully used in cases where zone electrophoresis fails. Advantages and drawbacks of these alternative separation techniques are discussed, as also are the difficulties encountered most often and solutions proposed by different research groups.

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Monolith and coating enzymatic microreactors of l-asparaginase: kinetics study by MCE–LIF for potential application in acute lymphoblastic leukemia (ALL) treatment

Cited by 25 publications

References 30 publications

Specific Anti-Leukemic Activity of the Peptide Warnericin RK and Analogues and Visualization of Their Effect on Cancer Cells by Chemical Raman Imaging

Specific Anti-Leukemic Activity of the Peptide Warnericin RK and Analogues and Visualization of Their Effect on Cancer Cells by Chemical Raman Imaging

Advances in Capillary Electrophoresis-Based Enzyme Assays

Microscale separation methods for enzyme kinetics assays

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