Monolayers of poly(γ-benzyl L-glutamate)/polyether block copolymers on the air/water interface

Cho, Chong‐Su; Nagata, R .; Yagawa, Akio; Takahashi, Sumika; Kunou, Megumi; Akaike, Toshihiro

doi:10.1002/pol.1990.140280303

Cited by 13 publications

(4 citation statements)

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“…A polymer solution of PMLG, Teco ex and HB-HV having a concentration of 0.1 wt% was spread onto the surface of ultrapure water in a LB trough (NL-LB200S-NWC, Nippon Laser & Electronics Lab., Nagoya, Japan) to form monolayers at 25 ± C [23]. For the preparation of collagen -PMLG LB lms, 80 dm 3 of 0.05 wt% collagen solution at pH 3.0 was inserted in a LB trough (Multicompartment trough [13,23]) and 0.1 wt% PMLG solution was spread onto the collagen solution to form a PMLG monolayer adsorbed on the collagen at 25 ± C. The polymer monolayer was transferred onto silicone-coated glass plates by a horizontal lifting method at 10 dyn cm ¡1 .…”

Section: Preparation Of Langmuir-blodgett Lmsmentioning

confidence: 99%

“…For the preparation of collagen -PMLG LB lms, 80 dm 3 of 0.05 wt% collagen solution at pH 3.0 was inserted in a LB trough (Multicompartment trough [13,23]) and 0.1 wt% PMLG solution was spread onto the collagen solution to form a PMLG monolayer adsorbed on the collagen at 25 ± C. The polymer monolayer was transferred onto silicone-coated glass plates by a horizontal lifting method at 10 dyn cm ¡1 .…”

Section: Preparation Of Langmuir-blodgett Lmsmentioning

confidence: 99%

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Growth of L929 cells on polymeric films prepared by Langmuir–Blodgett and casting methods

Higuchi

Tamiya

Tsubomura

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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The growth and spreading of fibroblast, L929 cells, on various polymeric films prepared by the Langmuir-Blodgett (LB) and casting methods were investigated. L929 cells, which were cultivated on collagen and synthetic polymeric films prepared by the LB method, adhered and spread much more than those on synthetic films prepared by the casting method. This is explained by the fact that cell growth and cell spreading are suitable for L929 cells on the films having serum proteins that contain a high alpha-helix content, because LB films adsorbed those serum proteins estimated from the circular dichroism measurements of the films immersed in cell culture medium. An exponential relationship was observed from the plot of the cell density vs root mean square of roughness of the films, which is estimated by atomic force microscopy, whereas a linear relationship was observed from the plot of the spreading ratio vs the root mean square of roughness. It is suggested that the correlation between the cell growth or spreading ratio and surface roughness of the films where L929 cells were cultivated is considered to be more important than the correlation between the cell growth or spreading ratio and the contact angle of the films.

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Section: Preparation Of Langmuir-blodgett Lmsmentioning

confidence: 99%

Section: Preparation Of Langmuir-blodgett Lmsmentioning

confidence: 99%

Growth of L929 cells on polymeric films prepared by Langmuir–Blodgett and casting methods

Higuchi

Tamiya

Tsubomura

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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“…LB technique has been an attractive tool for arranging various molecules into welldefined ordered forms to develop ultrathin films for electronic and photoelectronic devices. 3,4 Typically, a monolayer is formed at the air/water interface, which is then converted into a condensed phase by compression, 5 and can be transferred by dipping and raising the substrate through the air/ water interface. LB films have layered structures with a welldefined order or orientation of the functional moieties.…”

Section: Lb Filmsmentioning

confidence: 99%

Preparation of polymeric self-assembly and its application to biomaterials

et al. 2003

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The self-assembly of polymers can lead to supramolecular systems and is related to the their functions of material and life sciences. In this article, self-assembly of Langmuir-Blodgett (LB) films, polymer micelles, and polymeric nanoparticles, and their biomedical applications are described. LB surfaces with a well-ordered and layered structure adhered more cells including platelet, hepatocyte, and fibroblast than the cast surfaces with microphase-separated domains. Extensive morphologic changes were observed in LB surface-adhered cells compared to the cast films. Amphiphilic block copolymers, consisting of poly(γ-benzyl L-glutamate) (PBLG) as the hydrophobic part and poly(ethylene oxide) (PEO) [or poly(N-isopropylacrylamide) (PNIPAAm)] as the hydrophilic one, can self-assemble in water to form nanoparticles presumed to be composed of the hydrophilic shell and hydrophobic core. The release characteristics of hydrophobic drugs from these polymeric nanoparticles were dependent on the drug loading contents and chain length of the hydrophobic part of the copolymers. Achiral hydrophobic merocyanine dyes (MDs) were self-assembled in copolymeric nanoparticles, which provided a chiral microenvironment as redshifted aggregates, and the circular dichroism (CD) of MD was induced in the self-assembled copolymeric nanoparticles.

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“…[14]. The block copolymer monolayers were transferred onto silane-treated glass plates by a horizontal lifting method.…”

Section: Measurement Of the Surface Pressurementioning

confidence: 99%

Difference in the attachment of hepatocytes between a poly(γ-benzyl L-glutamate) (PBLG)/poly(N-isopropylacrylamide) (PNIPAAm) diblock copolymer cast surface and a PBLG/PNIPAAm Langmuir-Blodgett one

Shimojo

Akaike

Hara

et al. 2002

Journal of Biomaterials Science, Polymer Edition

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The effects of temperature on the monolayer behavior of the poly(gamma-benzyl L-glutamate) (PBLG)/poly(N-isopropylacrylamide) (PNIPAAm) diblock copolymer at the air-water interface were examined. Differences in the adhesion and morphology of hepatocytes between Langmuir-Blodgett (LB) films and cast surfaces of the PBLG/PNIPAAm diblock copolymer were investigated. The surface pressure (pi)-area (A) curve of the block copolymer had a tendency to expand with the temperature, due to a change in the conformation of PNIPAAm with the temperature change. Attachment of hepatocytes onto the PBLG/PNIPAAm block copolymer LB surface decreased slightly with an increase of the PNIPAAm content in the block copolymer, whereas that onto the cast surface decreased rapidly with an increase of the PNIPAAm content, due to the hydrophilic property of PNIPAAm in the microphase-separated structure. Rapid morphological changes of the hepatocytes adhered to the LB surfaces, from round shapes to spreading ones, were observed, compared with the cast films. The hepatocytes that adhered to the block copolymer LB surfaces showed less flattened and spread shapes than those that adhered to the PBLG one. Also, the spheroid formation of the hepatocytes increased with an increase of the PNIPAAm content in the block copolymer cast films.

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Monolayers of poly(γ-benzyl L-glutamate)/polyether block copolymers on the air/water interface

Cited by 13 publications

References 0 publications

Growth of L929 cells on polymeric films prepared by Langmuir–Blodgett and casting methods

Growth of L929 cells on polymeric films prepared by Langmuir–Blodgett and casting methods

Preparation of polymeric self-assembly and its application to biomaterials

Difference in the attachment of hepatocytes between a poly(γ-benzyl L-glutamate) (PBLG)/poly(N-isopropylacrylamide) (PNIPAAm) diblock copolymer cast surface and a PBLG/PNIPAAm Langmuir-Blodgett one

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